start-ver=1.4
cd-journal=joma
no-vol=134
cd-vols=
no-issue=2
article-no=
start-page=516
end-page=520
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080530
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Oxygen electrode as a new tool to evaluate hydroxyl radical-scavenging ability
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>An oxygen electrode was applied to determine hydroxyl radical ((OH)-O-center dot) levels for the first time. The method is based on the determination of (OH)-O-center dot generated by the Fenton reaction using the reaction of (OH)-O-center dot with a scavenger and the resulting radical consuming an oxygen molecule stoichiometrically. Thus, the (OH)-O-center dot-scavenging abilities of antioxidant reagents, as well as the concentration of (OH)-O-center dot, can be determined by the measurement of consumption of dissolved oxygen using an oxygen electrode. A good correlation between the present method and conventional colorimetry was obtained for the estimation of the (OH)-O-center dot-scavenging activities of antioxidants. Furthermore, the results correlated with the (OH)-O-center dot-scavenging rate constants of the reagents evaluated by a "cupric ion reducing antioxidant capacity (CUPRAC)" assay. We applied the present method to estimate the (OH)-O-center dot-scavenging abilities of commercially available alcoholic drinks.</p>
en-copyright=
kn-copyright=

en-aut-name=KomagoeKeiko
en-aut-sei=Komagoe
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakeuchiHiroaki
en-aut-sei=Takeuchi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatsuTakashi
en-aut-sei=Katsu
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=Oxygen electrode
kn-keyword=Oxygen electrode
en-keyword=Oxygen consumption
kn-keyword=Oxygen consumption
en-keyword=Hydroxyl radical
kn-keyword=Hydroxyl radical
en-keyword=Hydroxyl radical-scavenger
kn-keyword=Hydroxyl radical-scavenger
en-keyword=Antioxidant
kn-keyword=Antioxidant
en-keyword=Alcoholic drink
kn-keyword=Alcoholic drink
END