start-ver=1.4
cd-journal=joma
no-vol=29
cd-vols=
no-issue=7
article-no=
start-page=1280
end-page=1286
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160517
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Expressions of lipoprotein receptors and cholesterol efflux regulatory proteins during luteolysis in bovine corpus luteum
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= The corpus luteum (CL) synthesises and secretes progesterone (P4), which is essential for the establishment and maintenance of pregnancy in mammals. P4 is synthesised from cholesterol. Cholesterol is internalised by low-density lipoprotein receptor (LDLR) and/or scavenger receptor B1 (SR-BI), and is effluxed by ATP-binding cassette (ABC) transporter A1 (ABCA1) and G1 (ABCG1). To test the hypothesis that lipoprotein receptors and ABC transporters are involved in functional luteolysis, we examined the expression of LDLR, SR-BI, ABCA1 and ABCG1 in bovine CL during the luteal stages and after injection of prostaglandin (PG) F2ƒ¿ on Day 10 after ovulation. Expression of LDLR and SR-BI mRNA and protein was lower in the regressed luteal than late luteal stage. Injection of cows with a PGF2ƒ¿ did not affect LDLR mRNA and protein levels in the CL. Although expression of SR-BI mRNA did not change, SR-BI protein expression decreased 12 and 24 h after PGF2ƒ¿ injection. The overall findings of the present study suggest that the decreased expression of SR-BI induced by PGF2ƒ¿ is one of the factors responsible for the continuous decrease in P4 production during functional luteolysis.
en-copyright=
kn-copyright=

en-aut-name=HorihataKei
en-aut-sei=Horihata
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YoshiokaShin
en-aut-sei=Yoshioka
en-aut-mei=Shin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SanoMasahiro
en-aut-sei=Sano
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YamamotoYuki
en-aut-sei=Yamamoto
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KimuraKoji
en-aut-sei=Kimura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SkarzynskiDariusz J.
en-aut-sei=Skarzynski
en-aut-mei=Dariusz J.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OkudaKiyoshi
en-aut-sei=Okuda
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University
kn-affil=

affil-num=2
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences
kn-affil=

affil-num=7
en-affil=Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University
kn-affil=
en-keyword=luteal phase
kn-keyword=luteal phase
en-keyword=ovary
kn-keyword=ovary
en-keyword=progesterone
kn-keyword=progesterone
en-keyword=prostaglandin
kn-keyword=prostaglandin
en-keyword=reproduction
kn-keyword=reproduction
END