start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=73 end-page=88 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140916 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Detection and analysis of non-retroviral RNA virus-like elements in plant, fungal, and insect genomes. en-subtitle= kn-subtitle= en-abstract= kn-abstract= Endogenous non-retroviral RNA like sequences (NRVSs) have been discovered in the genome of a wide range of eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms, and in many cases, those fossils are estimated to be millions-of-years-old. It is likely that the number of NRVS records will increase rapidly due to the growing availability of whole-genome sequences for many kinds of eukaryotes. Discovery of the novel NRVSs and understanding of their phylogenetic relationship with modern viral relatives provide important information on deep evolutionary history of RNA virus-host interactions. In this chapter, therefore, the common strategies for the identification and characterization of endogenous NRVSs from plants, insects, and fungi are described. en-copyright= kn-copyright= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ChibaSotaro en-aut-sei=Chiba en-aut-mei=Sotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuzukiNobuhiro en-aut-sei=Suzuki en-aut-mei=Nobuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources (IPSR)Okayama University kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= en-keyword=Pareovirology kn-keyword=Pareovirology en-keyword=Molecular fossil record kn-keyword=Molecular fossil record en-keyword=Non-retrovirus-like sequence kn-keyword=Non-retrovirus-like sequence en-keyword=Database search kn-keyword=Database search en-keyword=Whole-genome shotgun kn-keyword=Whole-genome shotgun en-keyword=Genomic PCR kn-keyword=Genomic PCR en-keyword=Southern blotting kn-keyword=Southern blotting en-keyword=Phylogenetic analysis kn-keyword=Phylogenetic analysis en-keyword=Maximum-likelihood kn-keyword=Maximum-likelihood END