start-ver=1.4 cd-journal=joma no-vol=156 cd-vols= no-issue=1 article-no= start-page=20 end-page=28 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201105 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comprehensive Sequence Analysis of 24,783 Barley Full-Length cDNAs Derived from 12 Clone Libraries en-subtitle= kn-subtitle= en-abstract= kn-abstract=Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future. en-copyright= kn-copyright= en-aut-name=MatsumotoTakashi en-aut-sei=Matsumoto en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaTsuyoshi en-aut-sei=Tanaka en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SakaiHiroaki en-aut-sei=Sakai en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AmanoNaoki en-aut-sei=Amano en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KanamoriHiroyuki en-aut-sei=Kanamori en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KuritaKanako en-aut-sei=Kurita en-aut-mei=Kanako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KikutaAri en-aut-sei=Kikuta en-aut-mei=Ari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KamiyaKozue en-aut-sei=Kamiya en-aut-mei=Kozue kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamamotoMayu en-aut-sei=Yamamoto en-aut-mei=Mayu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IkawaHiroshi en-aut-sei=Ikawa en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=FujiiNobuyuki en-aut-sei=Fujii en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=HoriKiyosumi en-aut-sei=Hori en-aut-mei=Kiyosumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=ItohTakeshi en-aut-sei=Itoh en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=SatoKazuhiro en-aut-sei=Sato en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=Natl Inst Agrobiol Sci affil-num=2 en-affil= kn-affil=Natl Inst Agrobiol Sci affil-num=3 en-affil= kn-affil=Natl Inst Agrobiol Sci affil-num=4 en-affil= kn-affil=Natl Inst Agrobiol Sci affil-num=5 en-affil= kn-affil=Inst Soc Techno Innovat Agr Forestry & Fisheries affil-num=6 en-affil= kn-affil=Inst Soc Techno Innovat Agr Forestry & Fisheries affil-num=7 en-affil= kn-affil=Inst Soc Techno Innovat Agr Forestry & Fisheries affil-num=8 en-affil= kn-affil=Inst Soc Techno Innovat Agr Forestry & Fisheries affil-num=9 en-affil= kn-affil=Inst Soc Techno Innovat Agr Forestry & Fisheries affil-num=10 en-affil= kn-affil=Inst Soc Techno Innovat Agr Forestry & Fisheries affil-num=11 en-affil= kn-affil=Hitachi Govt & Publ Corp Syst Engn Ltd affil-num=12 en-affil= kn-affil=Natl Inst Agrobiol Sci affil-num=13 en-affil= kn-affil=Natl Inst Agrobiol Sci affil-num=14 en-affil= kn-affil=Okayama Univ END