start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=22
article-no=
start-page=e14640
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201123
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=After parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (M Phi s) to the subepithelial stroma. These attracted M Phi s are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5 degrees C) and HS conditions (40.5 degrees C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p < .05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p < .05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p < .05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of M Phi s; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p < .05) attracted the M Phi s when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of M Phi s in the endometrium during the summer.
en-copyright=
kn-copyright=

en-aut-name=SakaiShunsuke
en-aut-sei=Sakai
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HatabuToshimitsu
en-aut-sei=Hatabu
en-aut-mei=Toshimitsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamamotoYuki
en-aut-sei=Yamamoto
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KimuraKoji
en-aut-sei=Kimura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=chemokine
kn-keyword=chemokine
en-keyword=cow
kn-keyword=cow
en-keyword=endometrial cells
kn-keyword=endometrial cells
en-keyword=endometritis
kn-keyword=endometritis
en-keyword=heat stress
kn-keyword=heat stress
END