start-ver=1.4
cd-journal=joma
no-vol=7
cd-vols=
no-issue=1
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120118
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=TRAPPC9 Mediates the Interaction between p150<sup>Glued</sup> and COPII Vesicles at the Target Membrane
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediate
compartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150<sup>Glued</sup>, a subunit of dynactin, has
been implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However,
whether and how COPII vesicle tether, TRAPP (Transport protein particle), plays a role in the interaction between COPII
vesicles and microtubules is currently unknown.
Principle Findings: We address the functional relationship between COPII tether TRAPP and dynactin. Overexpressed TRAPP
subunits interfered with microtubule architecture by competing p150<sup>Glued</sup> away from the MTOC. TRAPP subunit TRAPPC9
bound directly to p150<sup>Glued</sup> via the same carboxyl terminal domain of p150<sup>Glued</sup> that binds Sec23 and Sec24. TRAPPC9 also
inhibited the interaction between p150<sup>Glued</sup> and Sec23/Sec24 both in vitro and in vivo, suggesting that TRAPPC9 serves to
uncouple p150<sup>Glued</sup> from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane.
Conclusions: These findings provide a new perspective on the function of TRAPP as an adaptor between the ERGIC
membrane and dynactin. By preserving the connection between dynactin and the tethered and/or fused vesicles, TRAPP
allows nascent ERGIC to continue the movement along the microtubules as they mature into the cis-Golgi.
en-copyright=
kn-copyright=

en-aut-name=ZongMin
en-aut-sei=Zong
en-aut-mei=Min
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SatohAyano
en-aut-sei=Satoh
en-aut-mei=Ayano
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YuMei Kuen
en-aut-sei=Yu
en-aut-mei=Mei Kuen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SiuKa Yu
en-aut-sei=Siu
en-aut-mei=Ka Yu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NgWing Yan
en-aut-sei=Ng
en-aut-mei=Wing Yan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=Cha‚ˇHsiao Chang
en-aut-sei=Cha‚ˇ
en-aut-mei=Hsiao Chang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TannerJulian A.
en-aut-sei=Tanner
en-aut-mei=Julian A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YuSidney
en-aut-sei=Yu
en-aut-mei=Sidney
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=School of Biomedical Sciences, The Chinese University of Hong Kong

affil-num=2
en-affil=
kn-affil=The Research Core for Interdisciplinary Science, Okayama University

affil-num=3
en-affil=
kn-affil=Epithelial Cell Biology Research Center, The Chinese University of Hong Kong,

affil-num=4
en-affil=
kn-affil=School of Biomedical Sciences, The Chinese University of Hong Kong

affil-num=5
en-affil=
kn-affil=School of Biomedical Sciences, The Chinese University of Hong Kong

affil-num=6
en-affil=
kn-affil=School of Biomedical Sciences, The Chinese University of Hong Kong

affil-num=7
en-affil=
kn-affil=Department of Biochemistry, The University of Hong Kong

affil-num=8
en-affil=
kn-affil=School of Biomedical Sciences, The Chinese University of Hong Kong
END