ID | 56657 |
FullText URL | |
Author |
Imamura, Daisuke
Collaborative Research Center of Okayama University for Infectious Diseases in India
Morita, Masatomo
Department of Bacteriology I, National Institute of Infectious Diseases
Sekizuka, Tsuyoshi
Pathogen Genomics Center, National Institute of Infectious Diseases
Mizuno, Tamaki
Collaborative Research Center of Okayama University for Infectious Diseases in India
Takemura, Taichiro
Vietnam Research Station, Institute of Tropical Medicine, Nagasaki University
Yamashiro, Tetsu
Vietnam Research Station, Institute of Tropical Medicine, Nagasaki University
Chowdhury, Goutam
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Pazhani, Gururaja P.
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Mukhopadhyay, Asish K.
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Ramamurthy, Thandavarayan
Translational Health Science and Technology Institute
Miyoshi, Shin-ichi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kuroda, Makoto
Pathogen Genomics Center, National Institute of Infectious Diseases
Shinoda, Sumio
Collaborative Research Center of Okayama University for Infectious Diseases in India
Ohnishi, Makoto
Department of Bacteriology I, National Institute of Infectious Diseases
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Abstract | Cholera is an acute diarrheal disease and a major public health problem in many developing countries in Asia, Africa, and Latin America. Since the Bay of Bengal is considered the epicenter for the seventh cholera pandemic, it is important to understand the genetic dynamism of Vibrio cholerae from Kolkata, as a representative of the Bengal region. We analyzed whole genome sequence data of V. cholerae O1 isolated from cholera patients in Kolkata, India, from 2007 to 2014 and identified the heterogeneous genomic region in these strains. In addition, we carried out a phylogenetic analysis based on the whole genome single nucleotide polymorphisms to determine the genetic lineage of strains in Kolkata. This analysis revealed the heterogeneity of the Vibrio seventh pandemic island (VSP)-II in Kolkata strains. The ctxB genotype was also heterogeneous and was highly related to VSP-II types. In addition, phylogenetic analysis revealed the shifts in predominant strains in Kolkata. Two distinct lineages, 1 and 2, were found between 2007 and 2010. However, the proportion changed markedly in 2010 and lineage 2 strains were predominant thereafter. Lineage 2 can be divided into four sublineages, I, II, III and IV. The results of this study indicate that lineages 1 and 2-I were concurrently prevalent between 2007 and 2009, and lineage 2-III observed in 2010, followed by the predominance of lineage 2-IV in 2011 and continued until 2014. Our findings demonstrate that the epidemic of cholera in Kolkata was caused by several distinct strains that have been constantly changing within the genetic lineages of V. cholerae O1 in recent years.
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Note | This research is supported by the Japan Initiative for Global Research Network on Infectious
Diseases (J-GRID) from Ministry of Education, Culture, Sport, Science & Technology in Japan, and Japan Agency for Medical Research and Development (AMED).
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Published Date | 2017-02-13
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Publication Title |
PLOS Neglected Tropical Diseases
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Volume | volume11
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Issue | issue2
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Publisher | PLOS
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Start Page | e0005386
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1371/journal.pntd.0005386
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Project |
Collaborative Research of Okayama University for Infectious Diseases in India
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