ID | 56674 |
FullText URL | |
Author |
Senoh, Mitsutoshi
Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Ghosh-Banerjee, Jayeeta
National Institute of Cholera and Enteric Diseases,
Mizuno, Tamaki
Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Shinoda, Sumio
Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
Miyoshi, Shin-ichi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Hamabata, Takashi
Research Institute, National Center for Global Health and Medicine
Nair, G. Balakrish
Translational Health Science and Technology Institute
Takeda, Yoshifumi
Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
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Abstract | Previously, we reported that viable but nonculturable (VBNC) Vibrio cholerae was converted into a culturable state by coculture with several eukaryotic cell lines including HT-29 cells. In this study, we found that a factor converting VBNC V. cholerae into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K-sensitive, and stable to heating at <60°C for 5 min. We prepared thiosulfate citrate bile salts sucrose (TCBS) plates with FCVC (F-TCBS plates). After confirming that VBNC V. cholerae O1 and O139 formed typical yellow colonies on F-TCBS plates, we tried to isolate cholera toxin gene-positive VBNC V. cholerae from environmental water samples collected in urban slum areas of Kolkata, India and succeeded in isolating V. cholerae O1 El Tor variant strains harboring a gene for the cholera toxin. The possible importance of VBNC V. cholerae O1 as a source of cholera outbreaks is discussed.
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Keywords | Factor converting VBNC into culturable
VBNC Vibrio cholerae
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Published Date | 2014-02-18
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Publication Title |
Microbiologyopen
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Volume | volume3
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Issue | issue2
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Publisher | Wiley-Blackwell
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Start Page | 239
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End Page | 246
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ISSN | 20458827
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1002/mbo3.164
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Project |
Collaborative Research of Okayama University for Infectious Diseases in India
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