start-ver=1.4
cd-journal=joma
no-vol=3
cd-vols=
no-issue=2
article-no=
start-page=239
end-page=246
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140218
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Previously, we reported that viable but nonculturable (VBNC) Vibrio cholerae was converted into a culturable state by coculture with several eukaryotic cell lines including HT-29 cells. In this study, we found that a factor converting VBNC V. cholerae into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K-sensitive, and stable to heating at <60°C for 5 min. We prepared thiosulfate citrate bile salts sucrose (TCBS) plates with FCVC (F-TCBS plates). After confirming that VBNC V. cholerae O1 and O139 formed typical yellow colonies on F-TCBS plates, we tried to isolate cholera toxin gene-positive VBNC V. cholerae from environmental water samples collected in urban slum areas of Kolkata, India and succeeded in isolating V. cholerae O1 El Tor variant strains harboring a gene for the cholera toxin. The possible importance of VBNC V. cholerae O1 as a source of cholera outbreaks is discussed.
en-copyright=
kn-copyright=

en-aut-name=SenohMitsutoshi
en-aut-sei=Senoh
en-aut-mei=Mitsutoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Ghosh-BanerjeeJayeeta
en-aut-sei=Ghosh-Banerjee
en-aut-mei=Jayeeta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MizunoTamaki
en-aut-sei=Mizuno
en-aut-mei=Tamaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ShinodaSumio
en-aut-sei=Shinoda
en-aut-mei=Sumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HamabataTakashi
en-aut-sei=Hamabata
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NairG. Balakrish
en-aut-sei=Nair
en-aut-mei=G. Balakrish
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TakedaYoshifumi
en-aut-sei=Takeda
en-aut-mei=Yoshifumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
kn-affil=

affil-num=2
en-affil=National Institute of Cholera and Enteric Diseases,
kn-affil=

affil-num=3
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
kn-affil=

affil-num=4
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Research Institute, National Center for Global Health and Medicine
kn-affil=

affil-num=7
en-affil=Translational Health Science and Technology Institute
kn-affil=

affil-num=8
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University
kn-affil=
en-keyword=Factor converting VBNC into culturable
kn-keyword=Factor converting VBNC into culturable
en-keyword=VBNC Vibrio cholerae
kn-keyword=VBNC Vibrio cholerae
END