start-ver=1.4
cd-journal=joma
no-vol=59
cd-vols=
no-issue=5
article-no=
start-page=305
end-page=310
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150209
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Stepwise changes in viable but nonculturable Vibrio cholerae cells
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.
en-copyright=
kn-copyright=

en-aut-name=ImamuraDaisuke
en-aut-sei=Imamura
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MizunoTamaki
en-aut-sei=Mizuno
en-aut-mei=Tamaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MiyoshiShin‐ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin‐ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ShinodaSumio
en-aut-sei=Shinoda
en-aut-mei=Sumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
kn-affil=

affil-num=2
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
kn-affil=

affil-num=3
en-affil=
kn-affil=

affil-num=4
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
kn-affil=
en-keyword=Vibrio cholerae
kn-keyword=Vibrio cholerae
en-keyword=resuscitation
kn-keyword=resuscitation
en-keyword=viable but nonculturable
kn-keyword=viable but nonculturable
END