start-ver=1.4
cd-journal=joma
no-vol=684
cd-vols=
no-issue=
article-no=
start-page=41
end-page=51
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=2011
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
en-copyright=
kn-copyright=

en-aut-name=ShenJian-Ren
en-aut-sei=Shen
en-aut-mei=Jian-Ren
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KawakamiKeisuke
en-aut-sei=Kawakami
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KoikeHiroyuki
en-aut-sei=Koike
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Department of Biosciences, Faculty of Science and Engineering, Chuo University
en-keyword=Photosystem II
kn-keyword=Photosystem II
en-keyword=Oxygen evolution
kn-keyword=Oxygen evolution
en-keyword=Crystallization
kn-keyword=Crystallization
en-keyword=Membrane proteins
kn-keyword=Membrane proteins
en-keyword=Ion-exchange chromatography
kn-keyword=Ion-exchange chromatography
END