start-ver=1.4
cd-journal=joma
no-vol=200
cd-vols=
no-issue=Part.A
article-no=
start-page=42
end-page=46
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A model-free method for extracting interaction potential between protein molecules using small-angle X-ray
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= A small-angle X-ray scattering has been used to probe protein–protein interaction in solution. Conventional methods need to input modeled potentials with variable/invariable parameters to reproduce the experimental structure factor. In the present study, a model-free method for extracting the excess part of effective interaction potential between protein molecules in solutions over an introduced hard-sphere potential by using experimental data of small-angle X-ray scattering is presented on the basis of liquid-state integral equation theory. The reliability of the model-free method is tested by the application to experimentally derived structure factors for dense lysozyme solutions with different solution conditions [Javid et al., Phys. Rev. Lett. 99, 028101 (2007), Schroer et al., Phys. Rev. Lett. 106, 178102 (2011)]. The structure factors calculated from the model-free method agree well with the experimental ones. The model-free method provides the following picture of the lysozyme solution: these are the stabilization of contact-pair configurations, large activation barrier against their formations, and screened Coulomb repulsion between the charged proteins. In addition, the model-free method will be useful to verify whether or not a model for colloidal system is acceptable to describing protein–protein interaction.
en-copyright=
kn-copyright=

en-aut-name=SumiTomonari
en-aut-sei=Sumi
en-aut-mei=Tomonari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ImamuraHiroshi
en-aut-sei=Imamura
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MoritaTakeshi
en-aut-sei=Morita
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NishikawaKeiko
en-aut-sei=Nishikawa
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Chemistry, Faculty of Science, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Advanced integration Science, Chiba University
kn-affil=

affil-num=3
en-affil=Graduate School of Advanced integration Science, Chiba University
kn-affil=

affil-num=4
en-affil=Graduate School of Advanced integration Science, Chiba University
kn-affil=
en-keyword=Protein solutions
kn-keyword=Protein solutions
en-keyword=Lysozyme
kn-keyword=Lysozyme
en-keyword=Protein-protein interaction
kn-keyword=Protein-protein interaction
en-keyword=Liquid state theory
kn-keyword=Liquid state theory
en-keyword=Integral equation
kn-keyword=Integral equation
en-keyword=DLVO model
kn-keyword=DLVO model
END