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Ikegame, Mika Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Ejiri, Sadakazu Department of Oral Anatomy, School of Dentistry, Asahi University
Okamura, Hirohiko Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Abstract
We investigated the influence of mechanical stretching on the genetic expression pattern of non-collagenous bone matrix proteins in osteoblasts. The cranial sutures of neonatal mice were subjected to ex vivo mechanical stretching. In the non-stretched control group, as osteoblast differentiation progressed, the successive genetic expression of bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN) was detected using in situ hybridization, in that order. In the stretched group, the sutures were widened, and after 24 hr of cultivation, a large number of osteoblasts and abundant new osteoid were observed on the borders of the parietal bones. All new osteoblasts expressed BSP and some of them expressed OPN, but very few of them expressed OCN. After 48 hr, more extensive presence of osteoid was noted on the borders of the parietal bones, and this osteoid was partially mineralized; all osteoblasts on the osteoid surface expressed BSP, and more osteoblasts expressed OPN than those after 24 hr cultivation. Surprisingly, many of the osteoblasts that did not express OPN, expressed OCN. This suggests that when osteoblast differentiation is stimulated by mechanical stress, the genetic expression pattern of non-collagenous proteins in the newly differentiated osteoblasts is affected.
Keywords
mechanical loading
non-collagenous bone proteins
ossification
sagittal sutures
Published Date
2018-08-16
Publication Title
Journal of Histochemistry and Cytochemistry
Volume
volume67
Issue
issue2
Publisher
Sage Publications
Start Page
107
End Page
116
ISSN
00221554
NCID
AA0069926X
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
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DOI
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isVersionOf https://doi.org/10.1369/0022155418793588
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Ikegame, M., Ejiri, S., & Okamura, H. (2019). Expression of Non-collagenous Bone Matrix Proteins in Osteoblasts Stimulated by Mechanical Stretching in the Cranial Suture of Neonatal Mice. Journal of Histochemistry & Cytochemistry, 67(2), 107–116. https://doi.org/10.1369/0022155418793588
Funder Name
Japan Society for the Promotion of Science
助成番号
14571729
16K11442