start-ver=1.4
cd-journal=joma
no-vol=179
cd-vols=
no-issue=2
article-no=
start-page=457
end-page=462
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=19940201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Anticardiolipin Antibodies Recognize β(2)-Glycoprotein I Structure Altered by Interacting with an Oxygen Modified Solid Phase Surface
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to beta(2)-glycoprotein I (beta(2)-GPI), which is adsorbed to an oxidized polystyrene surface. Oxygen atoms were introduced on a polystyrene surface by irradiation with electron or gamma-ray radiation. X-ray photoelectron spectroscopy revealed the irradiated surfaces were oxidized to generate C-O and C=O moieties. aCL derived from either APS patients or (NZW x BXSB)F-1 mice bound to beta(2)-GPI coated on the irradiated plates, depending on the radiation dose. Antibody binding to beta(2)-GPI on the irradiated plates was competitively inhibited by simultaneous addition of cardiolipin (CL)-coated latex beads mixed together with beta(2)-GPI but were unaffected by addition of excess beta(2)-GPI, CL micelles, or CL-coated latex beads alone. There was a high correlation between binding values of aCL in sera from 40 APS patients obtained by the anti-beta(2)-GPI enzyme-linked immunosorbent assay (ELISA) using the irradiated plates and those by the beta(2)-GPI-dependent aCL ELISA. Therefore, aCL have specificity for an epitope on beta(2)-GPI. This epitope is expressed by a conformational change occurring when beta(2)-GPI interacts with an oxygen-substituted solid phase surface.
en-copyright=
kn-copyright=

en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IgarashiYoshiko
en-aut-sei=Igarashi
en-aut-mei=Yoshiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YasudaTatsuji
en-aut-sei=Yasuda
en-aut-mei=Tatsuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TriplettDouglas A.
en-aut-sei=Triplett
en-aut-mei=Douglas A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KoikeTakao
en-aut-sei=Koike
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Cell Chemistry, Institute of Molecular and Cellular Biology, Okayama University School of Medicine

affil-num=2
en-affil=
kn-affil=Immunology Laboratory, Diagnostics Division, Yamasa Corporation

affil-num=3
en-affil=
kn-affil=Department of Cell Chemistry, Institute of Molecular and Cellular Biology, Okayama University School of Medicine

affil-num=4
en-affil=
kn-affil=Department of Pathology, Ball Memorial Hospital

affil-num=5
en-affil=
kn-affil=Department of Medicine II, Hokkaido University School of Medicine
END