start-ver=1.4
cd-journal=joma
no-vol=185
cd-vols=
no-issue=
article-no=
start-page=57
end-page=62
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A rapid and enhanced DNA detection method for crop cultivar discrimination
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products.
en-copyright=
kn-copyright=

en-aut-name=MondenYuki
en-aut-sei=Monden
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakasakiKazuto
en-aut-sei=Takasaki
en-aut-mei=Kazuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FutoSatoshi
en-aut-sei=Futo
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NiwaKousuke
en-aut-sei=Niwa
en-aut-mei=Kousuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KawaseMitsuo
en-aut-sei=Kawase
en-aut-mei=Mitsuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AkitakeHiroto
en-aut-sei=Akitake
en-aut-mei=Hiroto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TaharaMakoto
en-aut-sei=Tahara
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University

affil-num=2
en-affil=
kn-affil=FASMAC Co., Ltd.

affil-num=3
en-affil=
kn-affil=FASMAC Co., Ltd.

affil-num=4
en-affil=
kn-affil=Graduate School of Biomedical Engineering, Tohoku University

affil-num=5
en-affil=
kn-affil=Graduate School of Biomedical Engineering, Tohoku University

affil-num=6
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University

affil-num=7
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University
en-keyword=Cultivar discrimination
kn-keyword=Cultivar discrimination
en-keyword=Multiplex PCR
kn-keyword=Multiplex PCR
en-keyword=Strawberry
kn-keyword=Strawberry
en-keyword=Practical application
kn-keyword=Practical application
en-keyword=Retrotransposon
kn-keyword=Retrotransposon
END