ID | 57385 |
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Author |
Kaneta, Takashi
Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
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Abstract | Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules. This review describes the CE-LIF methods developed by the author's group that include indirect and direct detection using diode lasers, post-column derivatization, and Hadamard transformation, as well as applications to the binding assays of specific DNA immunoassays of proteins and to the determination of anticancer drugs.
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Keywords | DNA sequencing
capillary electrophoresis
laser-induced fluorescence
micellar electrokinetic chromatography
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Published Date | 2018-08-06
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Publication Title |
Chemical Record
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Volume | volume19
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Issue | issue2-3
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Publisher | Wiley
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Start Page | 452
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End Page | 461
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ISSN | 15278999
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NCID | AA11515019
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1002/tcr.201800051
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