start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue=6 article-no= start-page=737 end-page=747 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130606 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Matrix-Embedded Osteocytes Regulate Mobilization of Hematopoietic Stem/Progenitor Cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=The bone marrow (BM) niche comprises multiple cell types that regulate hematopoietic stem/progenitor cell (HSPC) migration out of the niche and into the circulation. Here, we demonstrate that osteocytes, the major cellular component of mature bone, are regulators of HSPC egress. Granulocyte colony-stimulating factor (G-CSF), used clinically to mobilize HSPCs, induces changes in the morphology and gene expression of the osteocytic network that precedes changes in osteoblasts. This rapid response is likely under control of the sympathetic nervous system, since osteocytes express the ƒÀ2-adrenergic receptor and surgical sympathectomy prevents it. Mice with targeted ablation of osteocytes or a disrupted osteocyte network have comparable numbers of HSPCs in the BM but fail to mobilize HSPCs in response to G-CSF. Taken together, these results indicate that the BM/bone niche interface is critically controlled from inside of the bone matrix and establish an important physiological role for skeletal tissues in hematopoietic function. en-copyright= kn-copyright= en-aut-name=AsadaNoboru en-aut-sei=Asada en-aut-mei=Noboru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KatayamaYoshio en-aut-sei=Katayama en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoMari en-aut-sei=Sato en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MinagawaKentaro en-aut-sei=Minagawa en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=WakahashiKanako en-aut-sei=Wakahashi en-aut-mei=Kanako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawanoHiroki en-aut-sei=Kawano en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KawanoYuko en-aut-sei=Kawano en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SadaAkiko en-aut-sei=Sada en-aut-mei=Akiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IkedaKyoji en-aut-sei=Ikeda en-aut-mei=Kyoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=MatsuiToshimitsu en-aut-sei=Matsui en-aut-mei=Toshimitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=TanimotoMitsune en-aut-sei=Tanimoto en-aut-mei=Mitsune kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=3 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=4 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=5 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=6 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=7 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=8 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=9 en-affil= kn-affil=Department of Bone and Joint Disease, National Center for Geriatrics and Gerontology (NCGG) affil-num=10 en-affil= kn-affil=Hematology, Department of Medicine, Kobe University Graduate School of Medicine affil-num=11 en-affil= kn-affil=Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences END