start-ver=1.4
cd-journal=joma
no-vol=28
cd-vols=
no-issue=5
article-no=
start-page=1362
end-page=1369
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190730
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Genome-Edited Triple-Recessive Mutation AltersSeed Dormancy in Wheat
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid wheat lines with loss of function of homeoalleles of Qsd1, which controls seed dormancy in barley, by Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed wheat events produced, three independent events carrying multiple mutations in wheat Qsd1 homeoalleles were obtained. Notably, one line had mutations in every homeoallele. We crossed this plant with wild-type cultivar Fielder to generate a transgene-free triple-recessive mutant, as revealed by Mendelian segregation. The mutant showed a significantly longer seed dormancy period than wild-type, which may result in reduced pre-harvest sprouting of grains on spikes. PCR, southern blotting, and whole-genome shotgun sequencing revealed that this segregant lacked transgenes in its genomic sequence. This technique serves as a model for trait improvement in wheat, particularly for genetically recessive traits, based on locus information from diploid barley.
en-copyright=
kn-copyright=

en-aut-name=AbeFumitaka
en-aut-sei=Abe
en-aut-mei=Fumitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HaqueEmdadul
en-aut-sei=Haque
en-aut-mei=Emdadul
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HisanoHiroshi
en-aut-sei=Hisano
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TanakaTsuyoshi
en-aut-sei=Tanaka
en-aut-mei=Tsuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KamiyaYoko
en-aut-sei=Kamiya
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MikamiMasafumi
en-aut-sei=Mikami
en-aut-mei=Masafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KawauraKanako
en-aut-sei=Kawaura
en-aut-mei=Kanako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=EndoMasaki
en-aut-sei=Endo
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OnishiKazumitsu
en-aut-sei=Onishi
en-aut-mei=Kazumitsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HayashiTakeshi
en-aut-sei=Hayashi
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SatoKazuhiro
en-aut-sei=Sato
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Division of Wheat and Barley Research, Institute of Crop Science, NARO
kn-affil=

affil-num=2
en-affil=Division of Wheat and Barley Research, Institute of Crop Science, NARO
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Division of Basic Research, Institute of Crop Science, NARO
kn-affil=

affil-num=5
en-affil=Kihara Institute for Biological Research, Yokohama City University
kn-affil=

affil-num=6
en-affil=Graduate School of Nanobioscience, Yokohama City University
kn-affil=

affil-num=7
en-affil=Kihara Institute for Biological Research, Yokohama City University
kn-affil=

affil-num=8
en-affil=Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
kn-affil=

affil-num=9
en-affil=Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine
kn-affil=

affil-num=10
en-affil=Division of Basic Research, Institute of Crop Science, NARO
kn-affil=

affil-num=11
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=CRISPR/Cas9
kn-keyword=CRISPR/Cas9
en-keyword=Qsd1
kn-keyword=Qsd1
en-keyword=multiple mutation
kn-keyword=multiple mutation
en-keyword=seed dormancy
kn-keyword=seed dormancy
en-keyword=wheat
kn-keyword=wheat
END