ID | 53018 |
FullText URL | |
Author |
Takiguchi, T
Yamamoto, T
Kajita, A
Ikeda, K
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Abstract | Background Cathelicidin antimicrobial peptide LL-37 has the capacity to kill a wide range of microbes and to modify host immunity. Recently, our group observed that the activation of keratinocytes by LL-37 and DNA greatly increases interferon (IFN)-beta through Toll-like receptor (TLR) 9. However, the effect of LL-37 on the induction of IFN-beta through TLR3, a sensor of double-stranded (ds) RNA, in keratinocytes is not well known.
Objectives To investigate whether LL-37 could affect TLR3 signalling and antiviral activity in normal human epidermal keratinocytes (NHEKs).
Methods We investigated the production of IFN-beta in NHEKs stimulated with a TLR3 ligand, poly (I:C), in the presence of LL-37. To examine the effect of LL-37 and poly (I:C) on antiviral activity, a virus plaque assay using herpes simplex (HS) virus type-1 was carried out. The uptake of poly (I:C) conjugated with fluorescein isothiocyanate (FITC) into the keratinocytes was observed in the presence of LL-37. Immunostaining for TLR3 and LL-37 was performed using skin samples from HS.
Results LL-37 and poly (I:C) synergistically induced the expression of IFN-beta in NHEKs. Furthermore, co-stimulation with LL-37 and poly (I:C) significantly decreased the viral plaque numbers compared with poly (I:C) or LL-37 alone. LL-37 enhanced the uptake of FITC-conjugated poly (I:C) into cells. Immunohistochemical analysis demonstrated that the expression of TLR3 and LL-37 is up-regulated in HS lesions.
Conclusions Our findings suggest that LL-37 augments the antiviral activity induced by dsRNA in keratinocytes, which may contribute to the innate immune response to cutaneous viral infections such as HS.
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Note | This is the accepted version of the following article: FULLCITE, which has been published in final form at http://dx.doi.org/10.1111/bjd.12942
学位審査副論文
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Published Date | 2014-09
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Publication Title |
British Journal of Dermatology
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Volume | volume171
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Issue | issue3
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Start Page | 492
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End Page | 498
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ISSN | 0007-0963
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2014 British Association of Dermatologists
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File Version | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/52974
http://ousar.lib.okayama-u.ac.jp/53764
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