start-ver=1.4
cd-journal=joma
no-vol=1774
cd-vols=
no-issue=4
article-no=
start-page=456
end-page=465
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=200704
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: The role of the C- and N-terminal extensions
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The aim of this study is to investigate the function of the C-terminal extension of three troponin I isoforms, that are unique to the body wall muscles of Caenorhabditis elegans and to understand the molecular interactions within the TN complex between troponin I with troponin C/T, and tropomyosin. We constructed several expression vectors to generate recombinant proteins of three body wall and one pharyngeal troponin I isoforms in Escherichia coli. Protein overlay assays and Western blot analyses were performed using antibodies. We demonstrated that pharyngeal TNI-4 interacted with only the pharyngeal isoforms of troponin C/T and tropomyosin. In contrast, the body wall TNI-2 bound both the body wall and pharyngeal isoforms of these components. Similar to other invertebrates, the N-terminus of troponin I contributes to interactions with troponin C. Full-length troponin I was essential for interactions with tropomyosin isoforms. Deletion of the C-terminal extension had no direct effect on the binding of the body wall troponin I to other muscle thin filament troponin C/T and tropomyosin isoforms.
en-copyright=
kn-copyright=

en-aut-name=AminMd. Ziaul
en-aut-sei=Amin
en-aut-mei=Md. Ziaul
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=BandoTetsuya
en-aut-sei=Bando
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=RuksanaRazia
en-aut-sei=Ruksana
en-aut-mei=Razia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Anokye-DansoFrederick
en-aut-sei=Anokye-Danso
en-aut-mei=Frederick
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakashimaYasuo
en-aut-sei=Takashima
en-aut-mei=Yasuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SakubeYasuji
en-aut-sei=Sakube
en-aut-mei=Yasuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KagawaHiroaki
en-aut-sei=Kagawa
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=4
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=5
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=6
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=7
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
en-keyword=Troponin I
kn-keyword=Troponin I
en-keyword=Caenorhabditis elegans
kn-keyword=Caenorhabditis elegans
en-keyword=Troponin T
kn-keyword=Troponin T
en-keyword=Troponin C
kn-keyword=Troponin C
en-keyword=Tropomyosin
kn-keyword=Tropomyosin
en-keyword=C-terminal extension
kn-keyword=C-terminal extension
END