ID | 57849 |
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Wei, Heng
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Wang, Chen
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Guo, Rui
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Takahashi, Ken
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Naruse, Keiji
Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract | Ischemic heart disease remains the largest cause of death worldwide. Accordingly, many researchers have sought curative options, often using laboratory animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart. In this study, we developed a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs). After optimizing the conditions of ischemia, including the concentration of oxygen and duration of application, we evaluated the consequent damage to hiPS-CMs. Notably, exposure to 2% oxygen, 0 mg/ml glucose, and 0% fetal bovine serum increased the percentage of nuclei stained with propidium iodide, an indicator of membrane damage, and decreased cellular viability. These conditions also decreased the contractility of hiPS-CMs. Furthermore, ischemic conditioning increased the mRNA expression of IL-8, consistent with observed conditions in the in vivo heart. Taken together, these findings suggest that our hiPS-CM-based model can provide a useful platform for human ischemic heart disease research.
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Keywords | Cardiomyocytes
Human induced pluripotent stem cells
Ischemic heart disease
Myocardial infarction
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Published Date | 2019-12-10
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Publication Title |
Biochemical and Biophysical Research Communications
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Volume | volume520
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Issue | issue3
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Publisher | Academic Press
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Start Page | 600
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End Page | 605
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ISSN | 0006291X
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NCID | AA00564395
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2019 The Authors.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1016/j.bbrc.2019.09.119
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License | http://creativecommons.org/licenses/by-nc-nd/4.0/
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Citation | Heng Wei, Chen Wang, Rui Guo, Ken Takahashi, Keiji Naruse, Development of a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells, Biochemical and Biophysical Research Communications, Volume 520, Issue 3, 2019, Pages 600-605, ISSN 0006-291X, https://doi.org/10.1016/j.bbrc.2019.09.119.
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Open Access (Publisher) |
OA
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