start-ver=1.4 cd-journal=joma no-vol=2011 cd-vols= no-issue=1 article-no= start-page=5 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110809 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Detection of in situ cleaved p115 with the cut specific antibodies in rapid protein inactivation system by tobacco etch viral protease cleavage en-subtitle= kn-subtitle= en-abstract= kn-abstract=Gene perturbation methods are commonly used in the study of gene and protein function. The authors of this paper recently developed a rapid protein inactivation technique utilizing tobacco etch virus (TEV)-derived protease. TEV protease recognizes the ENLYFQG (Glu-Asn-Leu-Tyr-Phe-Gln-Gly) amino acid sequence and specifically cleaves between Q and G. The authors developed antibodies that recognize the cleaved TEV (ENLYFQ) sequence, both in vitro and in vivo, but do not bind to uncleaved TEV (ENLYFQG). Using these antibodies, in situ protein cleavage was successfully detected. These antibodies used in combination with the TEV protease may be a useful complement to other perturbation methods. en-copyright= kn-copyright= en-aut-name=KoreishiMayuko en-aut-sei=Koreishi en-aut-mei=Mayuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HonjoYasuko en-aut-sei=Honjo en-aut-mei=Yasuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatohAyano en-aut-sei=Satoh en-aut-mei=Ayano kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=The Research Core for Interdisciplinary Sciences (RCIS), Okayama University affil-num=2 en-affil= kn-affil=The Research Core for Interdisciplinary Sciences (RCIS), Okayama University affil-num=3 en-affil= kn-affil=The Research Core for Interdisciplinary Sciences (RCIS), Okayama University en-keyword=TEV protease kn-keyword=TEV protease en-keyword=Golgi kn-keyword=Golgi en-keyword=golgins kn-keyword=golgins en-keyword=microinjection kn-keyword=microinjection en-keyword=recombinant proteins kn-keyword=recombinant proteins END