start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=5
article-no=
start-page=619
end-page=625
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=19921010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Separation and Determination of Alkaline-Earth Metal Ions as UV- Absorbing Chelates with EDTA by Capillary Electrophoresis. Determination of Calcium and Magnesium in Water and Serum Samples
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Capillary electrophoresis of alkaline-earth metal ions was examined with a UV-absorbing chelating agent. The metal1 chelates separated in a capillary were measured by on-column UV-absorptive detection. When ethylenediamine-tetraacetic acid (EDTA) was used as an chelating agent in a carrier solution (pH 9.2), the order of the migration time (t(m)) of metal ions was as follows: Ba(2+)<Sr(2+)<Ca(2+)<Mg(2+) (Be(2+) could not be detected). These four ions were separated within 16min using a capillary (50μm i.d.) of 75-cm effective length (L(D)) at an applied voltage of 30kV, and calcium and magnesium ions were separated within 4min using a 25-cm capillary at an applied voltage of 20kV. By using a 50-cm or 25-cm capillary, Ca(2+) and Mg(2+) in river, tap and underground water samples were determined; the detection limits of metal ions were about .10(-5)M (1M=mol dm(-3)), and the relative standard deviations for the determination of around 10(-4)M of the metal ions were less than 2.0%. The method was also applied to the determination of Ca(2+) and Mg(2+) in a standard serum sample.
en-copyright=
kn-copyright=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=1
ORCID=

en-aut-name=OshimaMitsuko
en-aut-sei=Oshima
en-aut-mei=Mitsuko
kn-aut-name=大島光子
kn-aut-sei=大島
kn-aut-mei=光子
aut-affil-num=2
ORCID=

en-aut-name=MatsudaShin-ya
en-aut-sei=Matsuda
en-aut-mei=Shin-ya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ObataYoshimitsu
en-aut-sei=Obata
en-aut-mei=Yoshimitsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TanakaHiroshi
en-aut-sei=Tanaka
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=Applied Biosystems Japan, Inc.
en-keyword=Alkaline-earth metal ion
kn-keyword=Alkaline-earth metal ion
en-keyword=water sample
kn-keyword=water sample
en-keyword=serum sample
kn-keyword=serum sample
en-keyword=EDTA
kn-keyword=EDTA
en-keyword=capillary electrophoresis
kn-keyword=capillary electrophoresis
en-keyword=on-column UV detection
kn-keyword=on-column UV detection
END