start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=11 article-no= start-page=2854 end-page=2859 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=201305 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Determination of polyamines in Arabidopsis thaliana by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent en-subtitle= kn-subtitle= en-abstract= kn-abstract=Herein, we report a novel method for the determination of polyamines in a sample extracted from Arabidopsis thaliana by capillary electrophoresis (CE) using salicylaldehyde-5-sulfonate (SAS) as a derivatizing reagent. An aldehyde group of SAS forms a Schiff base with amino groups of aliphatic polyamines, resulting in an anionic species with an absorption band in the ultraviolet region. The derivatization method was straightforward since the derivatives were formed by mixing a sample with the derivatizing reagent at a neutral pH. In addition, the negative charges induced by SAS led to a high resolution with a short analysis time. This method permitted the separation of five polyamines, which play important roles in plants. However, further improvement in sensitivity was needed for the determination of the polyamines in plant samples. Therefore, the CE method was coupled with solid-phase extraction (SPE) using an ion-pairing formation with sodium dodecyl benzene sulfonate. The SPE method improved the concentration limits of detection to sub-μM levels, which corresponded with a 10-fold enhancement. The calibration curves for cadaverine, putrescine, and spermidine were linear with concentrations that ranged from 1 to 20 μM and correlation coefficients (R2) were greater than 0.998. The proposed method was applied to the determination of spermidine in a plant sample, Arabidopsis thaliana. en-copyright= kn-copyright= en-aut-name=InoueGenki en-aut-sei=Inoue en-aut-mei=Genki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KanetaTakashi en-aut-sei=Kaneta en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KakehiJunichi en-aut-sei=Kakehi en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MotoseHiroyasu en-aut-sei=Motose en-aut-mei=Hiroyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakahashiTaku en-aut-sei=Takahashi en-aut-mei=Taku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Department of Life System, Institute of Technology and Science, The University of Tokushima affil-num=4 en-affil= kn-affil=Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University END