このエントリーをはてなブックマークに追加
ID 48104
FullText URL
Title Alternative
The Effect of Endotoxin on the Neutrophil Chemiluminescence in the Rabbit
Author
Asao, Yasuhiro
Abstract
好中球の貪食殺菌能を調べる方法として,増感剤を用いた化学発光(CL)法がある。好中球CLは細菌感染によって増大するが,重症感染症になると低下する場合がある。重症の細菌感染症における好中球CLの低下の原因を探るために,家兎に高濃度(70μg・kg(-1):III群)あるいは低濃度エンドトキシン(0.7μg・kg(-1):II群)を一回静注し,好中球の貪食殺菌能をCL法によって経時的に測定した。好中球を用いたCLピーク値は,II群では180分,360分で有意の上昇が認められ,III群では30分,60分,180分で有意の低下が認められた。好中球とプール血漿を組み合わせてCLを測定したところ,類似した結果が得られたため,好中球側に原因があることが示唆された。全血を用いたCLピーク値と白血球数とは類似の変化を示し,30分で有意に低下し,60分で最低を示したのちに回復した。以上より,軽度のエンドトキシン血症時には,好中球自体の貪食殺菌能が亢進する。しかし,重症エンドトキシン血症時には好中球数減少に伴う生体側の殺菌能の低下のほかに,好中球自体の貪食殺菌能の抑制が起こることが示された。
Abstract Alternative
To examine the effects of endotoxin on the neutrophil function, neutrophil-dependent microbicidal activities were evaluated by the chemiluminescence (CL) method in rabbits injected with endotoxin. Six rabbits in the first group were administered saline intravenously as control. Ten rabbits each in the second and third group received endotoxin 0.7 μg・kg(-1) and 70 μg・kg(-1) respectively. Luminol-dependent CL in whole blood (whole blood CL) and in isolated neutrophils (neutrophil CL) were examined before administration and 30, 60, 180 and 360 min after administration. Peak values of whole blood CL in the second group were significantly depressed at 30 and 60 min, and elevated at 360 min. Those in the third group were depressed more than in the second group at all times after endotoxin administration. Peak values of neutrophil CL in the second group at 180 and 360 min were significantly elevated, but those in the third group at 30, 60, 180 min were markedly depressed. Experiments combining neutrophils and pooled plasma revealed that these CL responses were associated with the neutrophils themselves. It is speculated that the neutrophil supression observed in the third group was caused by the overactivation of ncutrophils induced by the activated complements and some other stimulating factors, and/or may be due to the abundant appearance of immature granulocytes with lesser activities.
Published Date
1994
Publication Title
麻酔
Publication Title Alternative
The Japanese Journal of Anesthesiology
Volume
volume43
Issue
issue1
Publisher
克誠堂出版株式会社
Start Page
18
End Page
26
ISSN
0021-4892
NCID
AN0023223X
Content Type
Journal Article
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/4671
language
日本語
File Version
publisher
Refereed
True