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ID 11768
Eprint ID
11768
FullText URL
K003329.pdf 75.2 KB
Title Alternative
ヘリコバクター・ピロリ熱ショック蛋白60は、単核球細胞中でのTLR2とMAPキナーゼ経路を介し,サイトカインIL-8の分泌を引き起こす
Author
Zhao, Ying
Abstract
Previous reports have indicated that Helicobacter pylori heat-shock protein 60 (H. pylori-HSP60), as an immunodominant antigen, induces interleukin (IL)-8 production in human monocytes. The exact mechanism by which H. pylori-HSP60 induces IL-8 production in monocytes has not been fully elucidated. In the present study, the downstream pathway by which H. pylori-HSP60 induces IL-8 secretion in human monocytic cell lines was investigated. Intact H. pylori, heat-killed H. pylori and H. pylori recombinant HSP60 (rHpHSP60) all induced the secretion of IL-8 and the activation of mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK) and p38, but not c-Jun N-terminal kinase (JNK), up to 24 h in NOMO1 cells. The specific inhibitors PD98059 and U0126 (for ERK1/2 signalling) and SB203580 (for p38 MAPK signalling) down-regulated IL-8 secretion from rHpHSP60-treated NOMO1 cells. An anti-Toll-like receptor (TLR)2 antibody or TLR2 small interfering RNA (siRNA) partially inhibited the secretion of IL-8, and anti-TLR2 antibody also suppressed activation of ERK and p38 MAPK in rHpHSP60-treated NOMO1 cells. These reactions were associated with nuclear factor-kappaB (NF-kappaB)-mediated transcriptional activation, since U0126, SB203580 and the anti-TLR2 antibody decreased NF-kappaB activation. Taken together, the results suggest that ERK and p38 MAPK signalling linked to the TLR2 recognition receptor in human monocytes may be an important pathway in H. pylori-HSP60-induced IL-8 secretion.
Keywords
GASTRIC EPITHELIAL-CELLS
CYTOKINE MESSENGER-RNA
HEAT-SHOCK PROTEINS
KAPPA-B ACTIVATION
PROINFLAMMATORY CYTOKINES
ENDOTHELIAL-CELLS
MONGOLIAN GERBILS
LYMPHOID-TISSUE
TLR 2
MACROPHAGES
Note
http://dx.doi.org/10.1099/jmm.0.46882-0
Published Date
2007-03-23
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第3329号
Granted Date
2007-03-23
Thesis Type
Doctor of Philosophy in Medical Science
Grantor
岡山大学
Official Url
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17244794 &dopt=Abstract
language
日本語
File Version
none
Refereed
Unknown