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ID 10613
Eprint ID
10613
FullText URL
Thumnail O004086.pdf 97.8 KB
Title Alternative
透過型電子顕微鏡法のためのマイクロウェーブ照射による試料内鉄コロイドの作製法
Author
Nakatani, Satoru
Abstract
We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.
Keywords
colloidal iron
microwave
histochemistry
transmission electron microscopy
Note
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16508690&dopt=Abstract
Published Date
2006-03-24
Publication Title
Content Type
Thesis or Dissertation
Grant Number
乙第4086号
Granted Date
2006-03-24
Thesis Type
博士(医学)
Grantor
岡山大学
Official Url
http://escholarship.lib.okayama-u.ac.jp/cgi/viewcontent.cgi?article=1041&context=amo
language
日本語
File Version
none
Refereed
Unknown