Acta Medica Okayama2005ADAMTS-9 Is Synergistically Induced by Interleukin-1β and Tumor Necrosis Factorα in OUMS-27 Chondrosarcoma Cells and in Human ChondrocytesENKadirDemircanNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Hepatocyte Growth Factor Attenuates Airway Hyperresponsiveness, Inflammation, and RemodelingENWataruItoNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Reduction of a VEGF Receptor, Flk-1, by Antisense Oligonucleotides Induces Motor Neuron Death in Rat Spinal Cord Exposed to HypoxiaENMitoShioteNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Effect of Vision on Postural Sway in Anterior Cruciate Ligament Injured KneesENKazuhiroOkudaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Regulation of synaptic vesicle recycling by calcineurin in different vesicle poolsENSusumuKumashiroNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Antitumor effect of gefitinib (`Iressa') on esophageal squamous cell carcinoma cell lines in vitro and in vivoENFumikataHaraNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Effects of hypothermia for a short period on histological outcome and extracellular glutamate concentration during and after cardiac arrest in ratsENKenTakataNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005A New HLA-DRB1 Genotyping Method Using Single Nucleotide Polymorphism (SNP) Analysis with Multiplex Primer Extension Reactions and Its Application to Mixed SamplesENKiyomiImabayashiNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Manganese superoxide dismutase overexpression changes plating efficiency bidirectionally according to change in redox for SaOS2 human osteosarcoma cell lineENMegumiKomatsuNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005XAGE-1 mRNA Expression in Prostate Cancer and Antibody Response in PatientsENFumihitoKoizumiNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Profile of Users of Prosthetic Limbs provided by the Fund of Law for the Welfare of Physically Disabled Persons in Okayama PrefectureENMasatoKotakemoriNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Relationship between two job stress models and coronary risk factors among Japanese part-time female employees of a retail companyENYukaKobayashiNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Determinants of Cisplatin and Irinotecan Activities in Human Lung Adenocarcinoma Cells: Evidence of Cisplatin Accumulation and TopoisomeraseⅠ ActivityENTadashiMatsumuraNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005In vivo, Real Time Visualization of the Interaction between Perialveolar Microcirculation and Individual Alveolar Respiration by CCD Videomicroscopy in RatsENTaroMorimotoNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Suppression of chondrosarcoma cells by 15-deoxy-Delta 12,14-prostaglandin J2 is associated with altered expression of Bax/Bcl-xL and p21ENZheng-NanShenWe previously reported that 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), the most potent agonist for peroxisome proliferator-activated receptor gamma (PPAR gamma), induces apoptosis of human chondrosarcoma cell line OUMS-27. The current study aimed to explore the mechanism of 15d-PGJ(2)-induced apoptosis and inhibition of cell proliferation in OUMS-27 cells. The preliminary results of cDNA microarray analysis showed the down-regulation of anti-apoptotic Bcl-xL and up-regulation of pro-apoptotic Bax in the process of 15d-PGJ(2)-induced apoptosis. These changes were further confirmed at mRNA and protein levels by RT-PCR and Western blot analysis, respectively. Among cyclin-dependent kinase inhibitors, p21 was induced and up-regulated by 15d-PGJ(2), but p16 and p27 were not changed, suggesting that the involvement of p21 in inhibition of cell proliferation. Activation of caspase-3 by 15d-PGJ(2) was partly, but not completely, blocked by PPAR gamma antagonist (GW9662) suggesting the 15d-PGJ(2) exerted its effect by PPAR gamma-dependent and -independent pathways. Interestingly, immunohistochemical study on human chondrosarcoma samples revealed that Bcl-xL is frequently expressed by tumor cells. The results of the current study suggest that the potential ability of 15d-PGJ(2) in regulation of cell cycle and inhibition of Bcl-xL expression might be beneficial in the development of novel pharmacological agents for chondrosarcoma.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Prevention of hemorrhagic shock-induced lung injury by heme arginate treatment in ratsENKyoichiroMaeshimaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Alcohols increase calmodulin affinity for Ca2+ and decrease target affinity for calmodulinENIchiroOhashiNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Usefulness of Helical Computed Tomography in Diagnosing Pulmonary Vein Stenosis in infantsENShinnichiOhtsukiNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Glutamine is a key regulator for amino acid-controlled cell growth through the mTOR signaling pathway in rat intestinal epithelial cellsENTohruNakajoNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Accommodative Lag under Habitual Seeing Conditions: Comparison Between Myopic and Emmetropic ChildrenENChiakiNakatsukaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Possible contribution of prior hepatitis B virus infection to the development of hepatocellular carcinomaENHironoriTanakaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Change in Parameters before and after Alpha-1-Blocker Therapy for Men with Lower Urinary Tract Symptoms Using Color Doppler Ultrasound Urodynamics: Possible Application for Prediction of Clinical OutcomeENYuichiWatanabeNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005The Rey-Osterrieth Complex Figure as a Measure of Executive Function in ChildhoodENKiyokoWatanabeNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Identification of Epigenetic Aberrant Promoter Methylation in Serum DNA Is Useful for Early Detection of Lung CancerENKeiichiFujiwaraNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Diagnostic capabilities of I-131, Tl-201, and Tc-99m-MIBI scintigraphy for metastatic differentiated thyroid carcinoma after total thyroidectomyENShunjiFujieNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Proteasome Inhibitors Can Alter the Signaling Pathways and Attenuate the P-Glycoprotein-Mediated Multidrug ResistanceENTakeoFujitaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005The NH2 Terminus of Influenza Virus Hemagglutinin-2 Subunit Peptides Enhances the Antitumor Potency of Polyarginine-mediated p53 Protein TransductionENHiroyukiMichiueNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005ACCURACY OF TWO NONINVASIVE METHODS OF DIAGNOSING BLADDER OUTLET OBSTRUCTION USING ULTRASONOGRAPHY: INTRAVESICAL PROSTATIC PROTRUSION AND VELOCITY-FLOW VIDEO URODYNAMICSENHiroyukiNoseNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005PREDICTING POSTOPERATIVE RENAL INSUFFICIENCY IN PATIENTS UNDERGOING NEPHRECTOMY FOR RENAL MALIGNANCY: ASSESSMENT BY RENAL SCINTIGRAPHY USING 99mTECHNETIUM- MERCAPTOACETYLTRIGLYCINEENYoshinoriShirasakiNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Characterization of the Bone Morphogenetic Protein (BMP) System in Human Pulmonary Arterial Smooth Muscle Cells Isolated from a Sporadic Case of Primary Pulmonary Hypertension: Roles of BMP Type IB Receptor (Activin Receptor-Like Kinase-6) in the Mitotic ActionENMasayaTakedaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Digitalradiographic Quantification of Myocardial Blood Flow Around a Transmyocardial Laser Channel in Rabbit HeartsENOsamiHonjoNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Stable long-term induction of perforin-positive CD8+ T cells in gut by oral administration of streptococcal preparation OK-432ENNorifumiMinoNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Osteogenic protein-1 reduces intercellular adhesion molecule-1 messenger RNA expression, infarct size and TUNEL-positive cardiomyocytes in ischemia / reperfusion rat heartsENJunichiHayashiNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Des-gamma-carboxy prothrombin is a potential autologous growth factor for hepatocellular carcinomaENMayumiSuzukiDes-gamma-carboxyl prothrombin (DCP) is a well recognized tumor marker for hepatocellular carcinoma (HCC). In the present study, we demonstrate that DCP has a mitogenic effect on HCC cell lines. Purified DCP stimulated DNA synthesis of Hep3B and SK-Hep-1 cells in a dose-dependent manner. DCP was found to bind with cell surface receptor Met causing Met autophosphorylation and also to activate STAT3 signaling pathway through Janus kinase 1. Luciferase gene reporter analysis showed that DCP induced STAT3-related transcription. Small interfering RNAs against both STAT3 and Met abrogated DCP-induced cell proliferation. DCP did not affect the mitogen-activated protein kinase pathway, Myc signaling pathway, or phosphoinositide 3-kinase/Akt pathway. Based on these results, we believe that DCP acts as an autologous mitogen for HCC cell lines. The Met-Janus kinase 1-STAT3 signaling pathway may be a major signaling pathway for DCP-induced cell proliferation.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Role of Macrophage Migration Inhibitory Factor in Otitis Media with Effusion in AdultsENShinKariyaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005The Relationship between Epidermal Growth Factor Receptor Mutations and Clinicopathologic Features in Non-Small Cell Lung CancersENMasakiTokumoPURPOSE: Recent studies reported that clinical responsiveness to gefitinib was associated with somatic mutation of epidermal growth factor receptor (EGFR) gene in non-small cell lung cancers (NSCLC). Here, we investigated the relationship between EGFR mutation and clinicopathologic features. EXPERIMENTAL DESIGN: EGFR mutational status of 120 NSCLCs was determined mainly in EGFR exons 18 to 21 by direct sequence and correlated with clinicopathologic parameters. RESULTS: EGFR mutations were present in 38 cases (32%) and the majority of mutations were in-frame deletions of exon 19 (19 cases) and a missense mutation in exon 21 (18 cases). EGFR mutations were frequently associated with adenocarcinoma (P < 0.0001), never smoker (P < 0.0001), and female gender (P = 0.0001). Of interest, increasing smoke exposure was inversely related to the rate of EGFR mutation (P < 0.0001). Multivariate analysis showed that smoking and histology were independent variables. Furthermore, gender difference was observed for the mutational location (P = 0.01) dominance of exon 19 for males and exon 21 for females. Twenty-one cases were treated with gefitinib and found that EGFR mutation was significantly related to gefitinib responsiveness (P = 0.002). In addition, median survival times of patients with and without EGFR mutations treated with gefitinib were 25.1 and 14.0 months, respectively. Patients with EGFR mutations had approximately 2-fold survival advantage; however, the difference was not significant. CONCLUSIONS: We show that EGFR mutations were significantly related to histology and smoke exposure and were a strong predictive factor for gefitinib responsiveness in NSCLC.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005Coronary Flow Reserve after Coronary Intervention is Similar in Patients with Preserved Viability in Previous Myocardial Infarction and in Those with Angina PectorisENAtsushiTakaishiNo potential conflict of interest relevant to this article was reported.