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ID 48822
FullText URL
Author
Zong, Min
Yu, Mei Kuen
Siu, Ka Yu
Ng, Wing Yan
Chan, Hsiao Chang
Tanner, Julian A.
Yu, Sidney
Abstract
Background: The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediate compartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150Glued, a subunit of dynactin, has been implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However, whether and how COPII vesicle tether, TRAPP (Transport protein particle), plays a role in the interaction between COPII vesicles and microtubules is currently unknown. Principle Findings: We address the functional relationship between COPII tether TRAPP and dynactin. Overexpressed TRAPP subunits interfered with microtubule architecture by competing p150Glued away from the MTOC. TRAPP subunit TRAPPC9 bound directly to p150Glued via the same carboxyl terminal domain of p150Glued that binds Sec23 and Sec24. TRAPPC9 also inhibited the interaction between p150Glued and Sec23/Sec24 both in vitro and in vivo, suggesting that TRAPPC9 serves to uncouple p150Glued from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane. Conclusions: These findings provide a new perspective on the function of TRAPP as an adaptor between the ERGIC membrane and dynactin. By preserving the connection between dynactin and the tethered and/or fused vesicles, TRAPP allows nascent ERGIC to continue the movement along the microtubules as they mature into the cis-Golgi.
Note
記事番号:e29995
Published Date
2012-01-18
Publication Title
PLoS ONE
Volume
volume7
Issue
issue1
ISSN
1932-6203
Content Type
Journal Article
Official Url
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0029995#top
language
英語
Copyright Holders
© 2012 Zong et al.
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publisher
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT
Pages
12