JaLCDOI 10.18926/AMO/32848
FullText URL fulltext.pdf
Author Huang, Zhenyu| Liu, Qingping| Li, Wenzhe| Wang, Renjun| Wang, Dan| Zhang, Yingbiao| Zhang, Fan| Chi, Yan| Liu, Zhe| Matsuura, Eiji| Liu, Zibo| Zhang, Qiming|
Abstract <p>We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.</p>
Keywords 7-KC NF-?B apoptosis atherosclerosis
Amo Type Original Article
Published Date 2010-04
Publication Title Acta Medica Okayama
Volume volume64
Issue issue2
Publisher Okayama University Medical School
Start Page 85
End Page 93
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20424663
Web of Science KeyUT 000276996900002