JaLCDOI 10.18926/AMO/32892
FullText URL fulltext.pdf
Author Takagi, Koji| Yamada, Teruo| Miki, Yukari| Umegaki, Teruo| Nishimura, Makoto| Sasaki, Junzo|
Abstract To clarify the development of follicular growth and atresia in the immature ovary, rats. ovaries and blood were removed at fixed points during the period from 0 to 35 days after birth (Day 0 to Day 35). The ovaries were immunohistochemically examined, and blood concentrations of serum follicle-stimulating hormone (FSH) and estrogen (E) were measured. We investigated how time-course changes in follicular cell proliferation, estrogen receptor β (ERβ), apoptosis, and FSH and E concentrations are connected with follicular growth and atresia. Apoptosis was found in the ova from Day 0 to Day 3. On Day 15, apoptosis occurred in some granulosa cell nuclei in some follicles, but BrdU uptake and the presence of cyclin D2 and ER β could be observed in other granulosa cells. From Day 17, apoptosis increased in the follicular granulosa cells, and BrdU uptake and the presence of cyclin D2 and ERβ were decreased. Follicular atresia continued, reaching a peak on Day 30. Serum FSH and E concentrations increased until Day 15, then markedly decreased after Day 17. The mechanism of apoptosis in the ova from Day 0 to 3 has not been clarified. However, the onset of follicular atresia was caused by apoptotic degeneration from Days 15 to 17. These results showed that the oocytes were selected by apoptosis at 2 points in the time-course of the maturation of the ovary.
Keywords histology apoptosis proliferation estrogen follicle-stimulating hormone
Amo Type Original Article
Published Date 2007-10
Publication Title Acta Medica Okayama
Volume volume61
Issue issue5
Publisher Okayama University Medical School
Start Page 283
End Page 298
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17971845
Web of Sience KeyUT 000250431700006
JaLCDOI 10.18926/AMO/32844
FullText URL fulltext.pdf
Author Honda, Mayumi| Yamada, Teruo| Nomura, Takako| Miki, Yukari| Kande, Shigeto| Seki, Akihiko| Sasaki, Junzou|
Abstract <p>Differential, histochemical and immunohistochemical changes were observed in hepatocytes from immediately to 7 days after isoflurane or sevoflurane exposure (at H 0 to on Day 7) to study the process of development and recovery in anesthetic-induced hepatic injury. A total of 570 7-week-old male Sprague-Dawley rats with or without phenobarbital treatment were exposed to isoflurane or sevoflurane in 100%, 21%, or 10% oxygen, or to 10% oxygen alone for 2h. In phenobarbital-treated rats, hepatocytes both with and without anesthetic exposure markedly changed in 10% oxygen at H 0. Glycogen and ribosomal ribonucleic acid (rRNA) disappeared at H 0 and at H 6, respectively, and at H 6, AST levels in the blood rose. From H 6 to Day 1, necrosis developed more markedly and widely in zone 3 hepatocytes exposed to anesthetics in 10% oxygen than in those exposed to oxygen alone. All degenerated tissues had returned to normal levels by day 7. Recovery of the hepatolobular structure may be attributed to rearrangement of remaining hepatocytes in the portal vein area. Both the disappearance of glycogen and rRNA and the increase in blood AST levels after exposure to isoflurane or sevoflurane are considered to be factors contributing to the induction of necrosis around the central vein. The grade of isoflurane-induced hepatic injury was found to be significantly higher than that of sevoflurane.</p>
Keywords isoflurane sevoflurane histochemistry hypoxia hepatic injury
Amo Type Article
Published Date 2003-02
Publication Title Acta Medica Okayama
Volume volume57
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 12
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12765218
Web of Sience KeyUT 000181198200001
JaLCDOI 10.18926/AMO/30998
FullText URL fulltext.pdf
Author Yamada, Teruo| Hirai, Yasuo| Sakano, Shigehiro| Kosaka, Makoto| Tada, Keiichi| Furutani, Sei| Kosaka, Futami|
Abstract <p>The direct determination by gas chromatography of blood levels of anesthetic agents has been difficult because of the water content of blood. In the present study, the method of Yokota et al. (1967) was modified by improving the packing materials of the column, the blood sample vaporizer and the flow-path during analysis. As a result, accurate and reproducible determination of halothane, enflurane and isoflurane dissolved in blood was achieved. With this system, blood in which halothane, enflurane and isoflurane had been dissolved could be analyzed without changing the column between samples. Moreover, each sample was prepared in less than 10 min, and more than 100 consecutive determinations could be made with excellent reproducibility. The coefficient of variation was less than 3.8%.</p>
Keywords determination halothane enflurance isoflurance gas chromatography
Amo Type Article
Published Date 1988-08
Publication Title Acta Medica Okayama
Volume volume42
Issue issue4
Publisher Okayama University Medical School
Start Page 183
End Page 192
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3177003
Web of Sience KeyUT A1988P884600001
JaLCDOI 10.18926/AMO/30686
FullText URL fulltext.pdf
Author Yamada, Teruo| Talbot, Alan| Iijima, Yoshio| Itano, Yoshitaro| Kosaka, Futami|
Abstract <p>This study used a Shimadzu IP-1B capillary type isotachophoretic apparatus with a potential gradient detector. An ipp-1 withdrawal cell was fitted to this and a technique for withdrawing individual components directly through this port was developed using a microsyringe. The recovery rate was up to 45% for individual target components. When 100% withdrawal of the target component was attempted by withdrawing a volume four times the calculated volume (so that the zones both before and after the target component were also included), the best recovery rate was only 78%. In all cases, the results varied less than 3%. The limit for analysis of individual components of a 0.01 M solution was around 3 microliters. If this volume was exceeded, the ion quantity was too large for the volume of the microcapillary tube and mixed zones formed such that complete separation and analysis of individual components became impossible.</p>
Keywords isotachophoresis potential gradient detector ion mobility ionic separation withdrawal cell
Amo Type Article
Published Date 1982-10
Publication Title Acta Medica Okayama
Volume volume36
Issue issue5
Publisher Okayama University Medical School
Start Page 399
End Page 406
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7180573
Web of Sience KeyUT A1982PN03500008
JaLCDOI 10.18926/AMO/30563
FullText URL fulltext.pdf
Author Yamada, Teruo| Talbot, Alan| Hirakawa, Misahisa| Kobayashi, Naohide| Shiwaku, Yoshitomo| Kosaka, Futami| Kimoto, Tetsuo|
Abstract Renal cortex was studied during experimentally induced ischemia. A transient increase in anerobic glycolysis occurred with concomitant swelling of both the Golgi apparatus and mitochondria. These intracytoplasmic organelles underwent marked changes in their intracellular positions. Infolding of cytoplasmic membrane at the basal side of proximal tubule cells increased in complexity and proceeded to enclose various intracytoplasmic microorganelles such as mitochondria and the Golgi apparatus. Piling up in layers was particularly marked around mitochondria. This piling up appeared as myelin-like structures on the free surface of, and within, proximal tubule cells, and followed disruption of the brush border at the free surface. Histological examination of thin sections showed that the fused portions of this brush border were actually brush border cytoplasmic membrane piled up in layers giving the appearance of myelin-like structures. After two hours of ischemia, parts of the membrane of these myelin-like structures were disrupted. Large vacuoles developed and these were thought to be related to the large vacuoles seen during cell degeneration.
Keywords Myelin-like structures ischemia kidney
Amo Type Article
Published Date 1980-02
Publication Title Acta Medica Okayama
Volume volume34
Issue issue1
Publisher Okayama University Medical School
Start Page 19
End Page 30
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6446836
Web of Sience KeyUT A1980JS13800003