JaLCDOI 10.18926/AMO/52790
FullText URL 68_4_243.pdf
Author Wada, Nozomu| Yasunaka, Tetsuya| Ikeda, Fusao| Nishina, Sohji| Korenaga, Masaaki| Hino, Keisuke| Fujioka, Shin-ichi| Osawa, Toshiya| Itoshima, Tatsuya| Kawanaka, Miwa| Yamada, Gotaro| Kariyama, Kazuya| Takayama, Hiroki| Kubota, Junichi| Morimoto, Yoichi| Mizushima, Takaaki| Yamashita, Haruhiko| Tanioka, Hiroaki| Negoro, Yuji| Toshimori, Junichi| Kobashi, Haruhiko| Hirano, Atsushi| Itano, Yasuo| Takaki, Akinobu| Yamamoto, Kazuhide|
Abstract Hepatitis B virus (HBV) is one of the major viruses causing acute hepatitis. Recently, the incidence of acute hepatitis with genotype A has been increasing in Japan. The aim of this study was to investigate acute hepatitis B (AHB) in Okayama prefecture, with special attention to HBV genotype A. AHB patients who visited one of 12 general hospitals in Okayama prefecture between 2006 and 2010 were retrospectively analyzed. Over the course of the study period, 128 patients were diagnosed with AHB. Sexual transmission was supposed in the majority of patients (78 patients, 61%), including 59 (76%) having sex with heterosexual partners. The genotypes of HBV were assessed in 90 patients (70%), of whom 27 patients were infected with genotype A, 5 with genotype B, and 58 with genotype C. The prevalence of genotype A was significantly higher among male patients (28.7%), aged 20-29 (35.6%, p<0.01), among men who had sex with men (100%, p<0.005), and among patients having sex with unspecified partners (44.8%, p<0.005). Genotype A was not a significant factor associated with delayed HBsAg disappearance. Caution should be exercised with regard to sexually transmissible diseases in order to slow the pandemic spread of AHB due to genotype A.
Keywords acute hepatitis hepatitis B virus
Amo Type Original Article
Published Date 2014-08
Publication Title Acta Medica Okayama
Volume volume68
Issue issue4
Publisher Okayama University Medical School
Start Page 243
End Page 247
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2014 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 25145410
Web of Sience KeyUT 000340687500006
Author Yamada, Gotaro|
Published Date 1974-02
Publication Title Acta Medica Okayama
Volume volume28
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/32660
FullText URL fulltext.pdf
Author Takahashi, Michiko| Yamada, Gotaro| Miyamoto, Rieko| Doi, Toshihiko| Endo, Hisashi| Nishimoto, Hiroshi| Fujiki, Shigeatsu| Shimomura, Hiroyuki| Mizuno, Motowo| Tsuji, Takao|
Abstract <p>We measured hepatitis C virus antibody titers in 13 patients with chronic hepatitis C to determine whether titration of hepatitis C virus antibody was useful or not, to predict and evaluate the efficacy of interferon (IFN) treatment. During administration of IFN, hepatitis C virus titers declined in all patients. Antibody titers performed before treatment as well as just at the end of treatment did not correlate with change of the alanine aminotransferase levels during administration of IFN. Antibody titers declined continuously after treatment in 5 patients with normal alanine amino-transferase levels for over 6 months after discontinuation of IFN. Antibody titers rose again in 6 patients whose alanine aminotransferase levels fluctuated after treatment. An exceptional pattern of change occurred in 2 patients whose antibody titers declined continuously although their alanine aminotransferase levels fluctuated after treatment. Repeated titration of hepatitis C virus antibody appears to be useful for evaluating the long-term efficacy of IFN treatment.</p>
Keywords titiration of hepatitis C virus antibody interferon chronic hepatitis C efficacy of treatment
Amo Type Article
Published Date 1992-10
Publication Title Acta Medica Okayama
Volume volume46
Issue issue5
Publisher Okayama University Medical School
Start Page 331
End Page 336
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1279945
Web of Sience KeyUT A1992JX49500003
JaLCDOI 10.18926/AMO32655
FullText URL fulltext.pdf
Author Endo, Hisashi| Yamada, Gotaro| Nakane, Paul K| Tsuji, Takao|
Abstract <p>To establish the most proper method of in situ hybridization in detection of HCV-RNA in the liver, various detailed procedures were examined using frozen as well as paraffin-embedded sections of tissue derived from patients. In frozen sections of the liver from hepatitis C patients obtained at autopsy or surgery, HCV-RNA was detectable by in situ hybridization using thymine-thymine dimerized oligonucleotide DNA probes when the sections were treated with ethanol-acetic acid at first, then 0.2 N hydrochloric acid, proteinase K (0.02 u/ml) and DNase. When the paraffin-embedded liver sections were used, more intense proteinase K treatment (0.2-2 u/ml) was required to expose viral RNA and even after that, the positive HCV-RNA signals were less than those in frozen sections, because the cytoplasmic RNA in the routine paraffin-embedded sections was preserved unevenly and less than in frozen sections. These findings indicate that in situ hybridization of HCV-RNA is useful for diagnosing HCV infection and should be a potent tool for monitoring the state of virus activities during therapy. However, the liver biopsy method should be modified so that RNA is retained properly to utilize biopsies more effectively for the routine diagnosis of HCV infection.</p>
Keywords hepatitis C virus RNA of hepatitis C virus in situ hybridzation thyminethymine dimer oligonucleotide DNA probe
Amo Type Article
Published Date 1992-10
Publication Title Acta Medica Okayama
Volume volume46
Issue issue5
Publisher Okayama University Medical School
Start Page 355
End Page 364
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1332425
Web of Sience KeyUT A1992JX49500006
JaLCDOI 10.18926/AMO/32203
FullText URL fulltext.pdf
Author Matsueda, Kazuhiro| Yamada, Gotaro| Tsuji, Takao|
Abstract <p>In order to clarify difference of the mucosal immunity in various sites of normal large and small intestines, we studied the population of lymphocyte subsets and immunoglobulin (Ig)-containing cells in situ in biopsy specimens taken from various sites (ascending colon, sigmoid colon and rectum) of the large intestine and from the duodenum using an immunohistochemical method. Monoclonal antibodies against pan-T (Leu 1), cytotoxic/suppressor T (Leu2a), helper/inducer T (Leu3a), suppressor T (Leu15) and natural killer/K (Leu7) cells, and polyclonal antibodies to human IgG, IgA and IgM were used. In the duodenum, intraepithelial lymphocytes (IELs) were more prominent than in the large intestine. Immunoelectron microscopic observation revealed that some Leu2a+ IELs possessed pseudopods extending into intestinal epithelial cells, indicating that some IELs belong to the cytotoxic T cell subset. Leu7+ IELs were scarcely observed and Leu7+/Leu1+ ratio was higher in the large intestine than in the duodenum. Furthermore, the number of Leu7+ cells were more in the distal than the proximal colon. In the lamina propria Ig-containing cells tended to be fewer in the rectum than in the duodenum and the proximal colon. Our findings may suggest the variation of local immune responses and the difference of assigned immunological functions among the various sites of the intestines.</p>
Keywords cytotoxic T cell subsets anti-Leu7(NK/K cells) immunoglobulin-containing cells intestinal mucosa
Amo Type Article
Published Date 1991-06
Publication Title Acta Medica Okayama
Volume volume45
Issue issue3
Publisher Okayama University Medical School
Start Page 161
End Page 169
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1891975
Web of Sience KeyUT A1991FV15000006
JaLCDOI 10.18926/AMO/31906
FullText URL fulltext.pdf
Author Yamada, Gotaro| Manabe, Koji| Nagashima, Hideo|
Abstract <p>Liver biopsy specimens obtained from a 31-year-old female with delta-positive hepatitis were studied by routine electron microscopy. In several nuclei of hepatocytes, there were filamentous or microtubular structures 15 to 20 nm in diameter, in the vicinity of which, round particles, probably cross sections of tubular ones, were seen. In these nuclei, irregular granules approximately 20 to 30 nm in diameter were also found in clusters. However, cores of Dane particles were not found in such hepatocytes. These intranuclear microtubular structures may be associated with delta agent.</p>
Keywords delta agent delta antigen intranuclear particles microtubular structures
Amo Type Article
Published Date 1986-08
Publication Title Acta Medica Okayama
Volume volume40
Issue issue4
Publisher Okayama University Medical School
Start Page 225
End Page 228
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3766206
Web of Sience KeyUT A1986D870500007
JaLCDOI 10.18926/AMO/31270
FullText URL fulltext.pdf
Author Mizuno, Motowo| Yamada, Gotaro| Sakamoto, Yuzi| Nishihara, Takashi| Yumoto, Yasuhiro| Moritsugu, Yasuo| Nagashima, Hideo|
Abstract <p>Serum specimens from 12 patients with type A hepatitis were analyzed for immunoglobulin M-type antibody to hepatitis A virus (IgM anti-HA). A recently developed solid-phase radioimmunoassay kit for IgM anti-HA (HAVAB-M, Abbott Laboratories) and a competitive binding radioimmunoassay kit (HAVAB, Abbott Laboratories) with or without 2-mercaptoethanol treatment, as modified by Yano et al. (Acta Hepatol. Jpn. 21, 704-712, 1980) were used to obtain an M-index. All specimens obtained within 60 days of the onset of illness and specimens from 2 of 4 patients later than 60 days after the onset were positive with the HAVAB-M test. This test gave negative results to sera which were positive for anti-HA by a standard HAVAB test in the following: 3 patients with type B hepatitis; 5 with non-A, non-B hepatitis; 11 healthy adults; and 10 sera strongly positive for rheumatoid factor. The M-index for type A hepatitis in sera within 30 days of the onset (mean value of the M-index, m, = 1.52; standard deviation, SD, = 0.25) was significantly higher than that for non-A hepatitis (m = 1.05; SD = 0.15) and for healthy adults (m = 1.02; SD = 0.10). The simplicity and usefulness of the HAVAB-M test in diagnosis of acute type A hepatitis over those measuring the M-index by HAVAB tests were shown by direct comparison of the results.</p>
Keywords type A hepatitis IgM anti-HA radioimmunoassay.
Amo Type Article
Published Date 1981-04
Publication Title Acta Medica Okayama
Volume volume35
Issue issue2
Publisher Okayama University Medical School
Start Page 77
End Page 84
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6269362
Web of Sience KeyUT A1981LS45700001
JaLCDOI 10.18926/AMO/31098
FullText URL fulltext.pdf
Author Takahashi, Michiko| Yamada, Gotaro| Doi, Toshihiko| Takatani, Masahiro| Kishi, Fumitoshi| Miyamoto, Rieko| Yoshizawa, Hiroshi| Okamoto, Hiroaki| Tsuji, Takao|
Abstract <p>Hepatitis C virus (HCV)-RNA in the blood was measured by polymerase chain reaction (PCR) in 37 subjects from eight families in which 2 or more persons tested seropositive for antibodies against C100-3 or CP9. HCV-RNA was positive in 17 of 37 subjects. Two or more HCV-RNA-positive subjects were observed in six of the families. Intrafamilial HCV infection was studied by determining the HCV-RNA type (I, II, III or IV) by PCR using type-specific primers. In two families, all of the subjects showed type III infection, and in three other families, all of the subjects showed type II infection, with different types of HCV infections being observed in only one family. The HCV type was uniform in all but one. These findings suggest a possibility of intrafamilial infection between husbands and wives and between members of the same household.</p>
Keywords HCV intrafamilial transmission HCV-RNA genotype
Amo Type Article
Published Date 1994-12
Publication Title Acta Medica Okayama
Volume volume48
Issue issue6
Publisher Okayama University Medical School
Start Page 293
End Page 297
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7535967
Web of Sience KeyUT A1994PZ34600002
JaLCDOI 10.18926/AMO/31096
FullText URL fulltext.pdf
Author Nishimoto, Hiroshi| Yamada, Gotaro| Mizuno, Motowo| Tsuji, Takao|
Abstract <p>We studied the distribution of class 1 and class 2 major histocompatibility complex (MHC) antigens on bile duct epithelial cells in liver from patients with primary biliary cirrhosis (PBC) by an immunohistochemical method using monoclonal antibodies to HLA-ABC products and HLA-D subregion products (HLA-DR, -DP, -DQ). By light microscopy, the expression of MHC class 1 antigens (HLA-ABC antigens) was enhanced in PBC compared with controls. While negligible staining of MHC class 2 antigens was detected on the bile duct in controls, de novo expression of MHC class 2 antigens, as well as the coexpression of HLA-DR, HLA-DQ, and HLA-DP antigens on the bile duct epithelial cells, was observed in PBC. By electron microscopy, HLA-ABC and HLA-DR antigens were present preferentially along the basolateral domain of the cell surface of the bile duct epithelial cells and on the membrane of the endoplasmic reticulum in the cytoplasm, suggesting that these MHC antigens are synthesized by the bile duct epithelial cells in PBC. The distribution of these MHC antigens on the basolateral surface of the bile duct epithelial cells, where they are easily accessible to immunocytes, supports the idea that MHC-restricted cytotoxic T lymphocytes are involved in the bile duct injury in PBC. </p>
Keywords MHC class I antigens MHC class 2 antigens bile duct epithelial cell primary biliary cirrhosis
Amo Type Article
Published Date 1994-12
Publication Title Acta Medica Okayama
Volume volume48
Issue issue6
Publisher Okayama University Medical School
Start Page 317
End Page 322
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7709759
Web of Sience KeyUT A1994PZ34600006
JaLCDOI 10.18926/AMO/30558
FullText URL fulltext.pdf
Author Yamada, Gotaro| Takahashi, Takashi| Mizuno, Motowo| Sakamoto, Yuji| Kaga, Kazumi| Kobayashi, Toshinari| Nagashima, Hideo|
Abstract <p>A recently modified method using peroxidase labeled antibodies for light and electron microscopic demonstration of hepatitis B virus (HBV) was applied to the evaluation of hepatitis B surface antigen (HBsAg) on the surface of liver cells in biopsy specimens from 24HBsAg chronic carriers. Membranous distribution of HBsAg was demonstrated in diffuse or scattered hepatocytes in all 4 asymptomatic carriers and in 3 of the 20 patients with HBsAg-positive chronic active hepatitis or liver cirrhosis. In these patients with membranous expression of HBsAg, hepatitis B e antigen, Dane particles and DNA polymerase were often detected in sera, and large amounts of hepatitis B core antigen appeared in the liver. These results suggest that membrane-bound HBsAg may be expressed by the HBV genome. The ultrastructural study of liver cells showing membranous expression disclosed dense deposits of reaction product indicative of HBsAg on the cell membrane and/or on assembled particles within the extracellular space. In some hepatocytes showing both diffuse cytoplasmic and membranous expression of HBsAg, HBsAg-positive membrane of cisternae open to the intercellular space was connected with the liver cell membrane. These findings supported the conjecture that HBV associated antigens are integrated into the liver cell membrane.</p>
Keywords type B hepatitis membrane-bound HBsAg immunoelectron microscopy peroxidase-labeled antibody method.
Amo Type Article
Published Date 1980-06
Publication Title Acta Medica Okayama
Volume volume34
Issue issue3
Publisher Okayama University Medical School
Start Page 175
End Page 187
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6447986
Web of Sience KeyUT A1980KE59200005
JaLCDOI 10.18926/AMO/30552
FullText URL fulltext.pdf
Author Mizuno, Motowo| Yamada, Gotaro| Sakamoto, Yuzi| Yumoto, Yasuhiro| Nagashima, Hideo|
Abstract <p>Antibody to hepatitis B core antigen (anti-HBc) was measured by radioimmunoassay using CORAB (Abbott Laboratories) in 10 cases of chronic persistent hepatitis (CPH), 46 cases of chronic aggressive hepatitis (CAH), 33 cases of liver cirrhosis (LC) and 53 cases of hepatocellular carcinoma (HCC) in relation to hepatitis B surface antigen (HBsAg) and its antibody (anti-HBs). Ninety-eight point four percent of patients with HBsAg and 93.8% of patients with anti-HBs were positive for anti-HBc and the titers of anti-HBc in patients with HBsAg were significantly higher than those with anti-HBs. Thirty-five point five percent of patients negative for either HBsAg or anti-HBs were positive for anti-HBc. The titers of anti-HBc in patients with CPH, CAH and LC were relatively low, whereas 7 (46.8%) of the HCC patients negative for either HBsAg or anti-HBc had high titers of anti-HBc. The significance of the presence of anti-HBc alone is discussed.</p>
Keywords anti-HBc HBsAg anti-HBs radioimmunoassay chronic liver disease.
Amo Type Article
Published Date 1980-06
Publication Title Acta Medica Okayama
Volume volume34
Issue issue3
Publisher Okayama University Medical School
Start Page 155
End Page 160
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6251699
Web of Sience KeyUT A1980KE59200002
JaLCDOI 10.18926/AMO/30540
FullText URL fulltext.pdf
Author Yamada, Gotaro| Sakamoto, Yuji| Mizuno, Motowo| Kobayashi, Toshinari| Nagashima, Hideo|
Abstract The liver biopsy specimen from a patient with hepatitis B surface antigen was pbserved by electron microscopy. Dane particles, uncoated core particles and tubular forms were demonstrated in hepatocytes. Dane particles and tubular forms, approximately 25nm in diameter, were also found in the bile canaliculi. These findings suggest that hepatitis B virus and associated particles are released from hepatocytes into the bile duct.
Keywords hepatitis B virus bile canaliculus.
Amo Type Brief Note
Published Date 1980-04
Publication Title Acta Medica Okayama
Volume volume34
Issue issue2
Publisher Okayama University Medical School
Start Page 143
End Page 146
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6450517
Web of Sience KeyUT A1980JY15600010
JaLCDOI 10.18926/AMO/30372
FullText URL fulltext.pdf
Author Fujiwara, Masachika| Watanabe, Akiharu| Sato, Yasumasa| Shiota, Tetsuya| Nakatsukasa, Harushige| Yamada, Gotaro| Nagashima, Hideo|
Abstract <p>Three patients with severe halothane-induced liver injury are described. All patients received halothane anesthesia twice within a short period. High fever and jaundice were noticed soon after the second operation. The prothrombin time was less than 40%, and eosinophilia was greater than 7% prior to these symptoms. Other causes of liver injury were excluded. Diagnostic criteria for halothane-induced liver injury are proposed.</p>
Keywords halothane liver injury eosinophilia
Amo Type Article
Published Date 1984-02
Publication Title Acta Medica Okayama
Volume volume38
Issue issue1
Publisher Okayama University Medical School
Start Page 35
End Page 40
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6702484
Web of Sience KeyUT A1984SF03000005
JaLCDOI 10.18926/AMO/30331
FullText URL fulltext.pdf
Author Yamada, Gotaro| Mizuno, Motowo| Kinoyama, Shingo| Nishihara, Takashi| Okushin, Hiroaki| Hyodo, Ichinosuke| Sakamoto, Yuji| Nagashima, Hideo|
Abstract <p>In 144 cases of hepatocellular carcinoma (HCC), 166 cases of cirrhosis without HCC and 142 cases of chronic hepatitis, we examined HBsAg, anti-HBs and anti-HBc in sera and compared the following factors between hepatitis B virus marker-negative and -positive patients: age, sex, alcohol consumption, family clustering of liver diseases, and histories of blood transfusion and post-transfusion hepatitis. Results of this study demonstrated several distinct differences in clinical backgrounds between non-B (negative for HBsAg, anti-HBs and anti-HBc) and B (positive for HBsAg) patients with HCC. Non-B patients were significantly older, had a lower frequency of familial tendencies for liver diseases, and more frequently had cancers other than HCC in their families. Some of these differences were also observed between non-B and B patients with cirrhosis and chronic hepatitis. Among patients with chronic hepatitis, the non-B patients had received blood transfusion or had post-transfusion hepatitis more frequently than the B patients. However, this difference was not apparent in patients with liver cirrhosis or HCC, suggesting that progression of non-A, non-B post-transfusion hepatitis to cirrhosis and HCC may not be as frequent as progression to chronic hepatitis.</p>
Keywords non-B hepatocellular carcinoma type non-A non-B hepatitis type B hepatitis post-transfusion hepatitis
Amo Type Article
Published Date 1984-04
Publication Title Acta Medica Okayama
Volume volume38
Issue issue2
Publisher Okayama University Medical School
Start Page 181
End Page 191
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6328894
Web of Sience KeyUT A1984SN81800010
JaLCDOI 10.18926/AMO/30310
FullText URL fulltext.pdf
Author Hyodo, Ichinosuke| Yamada, Gotaro| Nishihara, Takashi| Okushin, Hiroaki| Kinoyama, Shingo| Sakamoto, Yuji| Tobe, Kazuo| Nagashima, Hideo|
Abstract <p>The incidence of hepatitis A (HA), hepatitis B (HB), and non-A, non-B hepatitis (NANBH) was 27%, 30% and 43% among 73 patients with sporadic hepatitis. Epidemiological data (geographical distribution, seasonal variation, age, sex, and occupation) were not distinguishing of the type of hepatitis. Neither intrafamilial infection nor previous contact with viral hepatitis patients could be demonstrated in the NANBH cases. Fever and jaundice were less frequent in NANBH than in HA. Maximum levels of SGPT, serum bilirubin, ZTT, and gamma-globulin were significantly lower in NANBH than in HA and HB. Ten of 29 NANBH patients (35%) presented abnormal SGPT activities for more than 6 months, and four (14%) more than 12 months. In the ten patients with prolonged courses, jaundice was more frequent and maximum levels of SGPT were higher than in patients with transient courses. Histopathologic findings were not markedly different from those of HA and HB. Bile duct damage, fatty deposition, and giant multi-nucleated cells were recognized in 6, 12, and 2 NANBH patients, respectively. There were no characteristic ultrastructural changes in NANBH.</p>
Keywords acute hepatitis sporadic non-A non-B hepatitis liver histopathology liver ultrastructure
Amo Type Article
Published Date 1984-08
Publication Title Acta Medica Okayama
Volume volume38
Issue issue4
Publisher Okayama University Medical School
Start Page 389
End Page 401
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6437147
Web of Sience KeyUT A1984TG25900009