JaLCDOI |
10.18926/AMO/32848
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FullText URL |
fulltext.pdf
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Author |
Huang, Zhenyu|
Liu, Qingping|
Li, Wenzhe|
Wang, Renjun|
Wang, Dan|
Zhang, Yingbiao|
Zhang, Fan|
Chi, Yan|
Liu, Zhe|
Matsuura, Eiji|
Liu, Zibo|
Zhang, Qiming|
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Abstract |
<p>We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.</p>
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Keywords |
7-KC
NF-?B
apoptosis
atherosclerosis
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Amo Type |
Original Article
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Published Date |
2010-04
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Publication Title |
Acta Medica Okayama
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Volume |
volume64
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Issue |
issue2
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Publisher |
Okayama University Medical School
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Start Page |
85
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End Page |
93
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ISSN |
0386-300X
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NCID |
AA00508441
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Content Type |
Journal Article
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language |
英語
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File Version |
publisher
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Refereed |
True
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PubMed ID |
20424663
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Web of Sience KeyUT |
000276996900002
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