| JaLCDOI | 10.18926/AMO/32848 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Huang, Zhenyu| Liu, Qingping| Li, Wenzhe| Wang, Renjun| Wang, Dan| Zhang, Yingbiao| Zhang, Fan| Chi, Yan| Liu, Zhe| Matsuura, Eiji| Liu, Zibo| Zhang, Qiming| |
| Abstract | <p>We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.</p> |
| Keywords | 7-KC NF-?B apoptosis atherosclerosis |
| Amo Type | Original Article |
| Published Date | 2010-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume64 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 85 |
| End Page | 93 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 20424663 |
| Web of Sience KeyUT | 000276996900002 |
