JaLCDOI 10.18926/19805
Title Alternative Clinical studies on bronchial asthma induced by chironomid midges -comparison between positive and negative cases to house dust-
FullText URL 060_054_060.pdf
Author Kitani, Hikaru| Araki, Hiroyuki| Sudo, Michiyasu| Tanizaki, Yoshiro| Tada, Sinya| Takahashi, Kiyoshi| Kimura, Ikuro| Matsuoka, Hiroyuki| Ishii, Akira|
Abstract 近年ユスリカが気管支喘息の原因抗原となり得ることが報告されている。しかし,その臨床的特徴はいまだ明らかにされていない。このユスリカ抗原の特徴を検討する目的で,気管支喘息症例163例をHD(ハウスダスト)に感作されたRAST score2+以上の陽性例82例とscore1+以下の陰性例81例とに分けて比較検討を行なった。その結果皮膚反応,ヒスタミン遊離,CTT抗原を用いた特異的IgE抗体いずれの検討においても,HDに感作された症例が,よりユスリカ抗原にも感作されやすいことが明らかになった。
Abstract Alternative In recent years it has been reported that chironomid migdes might be one of the causative allergens in bronchial asthma. In order to clarify the clinical characteristics of bronchial asthma induced by chironomid midges (Tokunagayusurika akamusi), 163 patients with bronchial asthma were studied. The subjects were divided into two groups ; cases with negative (0~1 + ) and positive (2 + or more) RAST score to house dust. 1. Skin reaction to midges was positive in 54 cases (33.1%) out of 163 patients with bronchial asthma. Thirty-six cases (43.9%) out of 82 asthmatics with positive RAST score to house dust showed positive skin reaction to midges, but in 81 cases with negative RAST score, only 18 cases (22.2%) showed positive skin reaction. 2. Fourteen cases out of 85 asthmatics showed a significant amount of histamine release (more than 15%). A significant amount of histamine release was observed in 12 cases (23.5%) out of 51 cases with positive RAST score, but in only two cases (5.9%) out of 24 cases with negative RAST score. 3. Specific IgE antibodies to CTT (Chiromonus thummi thummi) was positive in 12 cases (18.2%) out of 66 asthmatics. In 41 asthmatics with positive RAST score to house dust, 12 cases (29.3%) showed positive RAST score to CTT, though in 25 cases with negative RAST score to house dust no cases showed positive RAST score to CTT. In conclusion, cases with house dust allergy were more easily sensitized by chironomid migdes than those cases without house dust allergy.
Keywords 気管支喘息 (Bronchial asthma) ユスリカ (Chironomid midges) ヒスタミン遊離 (Histamine release) ハウスダスト (House dust)
Publication Title 環境病態研報告
Published Date 1989-07
Volume volume60
Start Page 54
End Page 60
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309057
JaLCDOI 10.18926/19802
Title Alternative IgE-mediated reaction in non-atopic asthma
FullText URL 060_047_053.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki|
Abstract 気管支喘息の発症にIgE抗体がどの程度関与しているのかを中心に若干の検討を加えた。1.若年発症型喘息(発症;20才以下,現年齢;30才以下)では,非難治例15例の血清IgE値は947±656IU/mℓで,HDのRASTが陽性を示した症例は12例(80%),難治例12例では血清IgE値214±102IU/mℓで,HDのRAST陽性は6例(50.0%)であった。2.一方,中高年発症型喘息(発症;40才以後)では,非難治例15例の血清IgE値は388±324IU/mℓで,HDのRAST陽性は6例(40%)であったが,難治例15例ではIgE値253±82IU/mℓで,HDのRASTは全例陰性であった。これらの結果から,いずれの群においても,IgE系反応が弱い場合に喘息が重症化しやすいことが示された。3.アトピー型,健康人,非アトピー型におけるI1gE系反応(皮内反応,血剤IgE,特異的IgE,ヒスタミン遊離)の陽性率や強度は,それぞれ異なっていたが,非アトピー型においてもIgE系反応が低下ないし抑制された状態で関与している可能性が示唆された。
Abstract Alternative IgE-mediated reaction was studied in non-atopic asthma in comparison with atopic asthma. 1. In early onset asthma patients (younger than 20 years at onset of the disease), the mean serum IgE level was 947±656IU/mℓ in non-intractable asthma cases and 214±102IU/mℓ in intractable cases. A positive RAST score to house dust was observed in 12 cases (80%) out of 15 non-intractable cases, and 6 (50%)out of 15 intractable cases. 2. In late onset asthma patients (older than 40 years at onset), the mean serum IgE level was 388±324IU/mℓ in non-intractable cases and 253±82IU/mℓ in intractable cases. There were no cases with a positive RAST score to house dust in intractable cases. These results may suggest that there is a correlation between decreased IgE-mediated reaction and a tendency to intractable asthma. 3. There were some differences in IgE mediated reaction among atopic, non-atopic asthma cases and healthy subjects, suggesting that IgE-mediated reaction may partially participate in the onset of non-atopic asthma in a suppressed condition.
Keywords アトピー型喘息 (Atopic asthma) 非アトピー型喘息 (Non-atopic asthma) 皮内反応 (Skin reaction)  血清IgE (Serum lgE) 特異的lgE (Specific lgE)
Publication Title 環境病態研報告
Published Date 1989-07
Volume volume60
Start Page 47
End Page 53
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309025
JaLCDOI 10.18926/19785
Title Alternative Spa therapy for bronchial asthma -Clinicai studies on 93 cases with bronchial asthma-
FullText URL 060_025_030.pdf
Author Sudo, Michiyasu| Araki, Hiroyuki| Kitani, Hikaru| Tanizaki, Yoshiro|
Abstract 1982年1月から1988年12月までの7年間に三朝分院内科に入院し,温泉療法を受けた気管支喘息93例を対象に,その背景因子,免疫アレルギー学的要素および温泉療法の臨床効果について総合的に検討した。1.年齢の平均は52.6才,発症年齢の平均は41.7才であり,両者ともやや高齢であった。2.血清IgE値の平均は506.2IU/mℓであった。300IU/mℓ以下の症例は59例(63.4%)であり,半数以上の症例が血清IgE値正常ないし低値を示した。3.皮内反応ではカンジダに陽性を示す症例が44例と最も多く,次にハウスダスト(以下HDと略す)26例,スギ13例,キヌ8例であった。4.換気機能検査では気管支攣縮型において換気機能の低下傾向が最も少なく,細気管支閉塞型で換気機能の低下傾向が特に% MMF,% V(50),% V(25)のような細気管支の閉塞を示すパラメーターの低下傾向がより高度であった。5.対象症例の臨床病型は気管支攣縮型が47例でほぼ対象症例の半数を占め次いで気管支攣縮+過分泌型29例,細気管支閉塞型17例の順であった。6.温泉療法の全般的有効率は79.6%であった。臨床病型別では細気管支閉塞型17例中16例(94.1%)が有効例であり,細気管支閉塞型で最も有効例がみられた。
Abstract Alternative Allergological characteristics and factors of spa therapy in relation to clinical effects were studied on 93 patients, who were adirnitted at Misasa Branch Hospital from 1982 to 1988. Fifty-seven out of 93 cases were steroid-dependent intractable asthma. 1. The mean age was 52.6 and the mean age at onset was 41.7 years old. The age and age at onset were higher ip these asthma cases. 2. The mean serum IgE levels was 506.2IU/mℓ. Serum IgE levels were generally low, and less than 300IU/mℓ in many cases. 3. Frequency of positive skin reactions to various allergens was generally low, and considerably higher in the two allergens, candida albicans and house dust. Cases with posiitive RAST score was most frequently observed in house dust, followed by candida albicans and J. cedar. 4. Ventilatory function tests of these patients showed that % V(50), % V(25)and % MMF which represent small airway obstruction were less decreased in bronchospasm type and most deereased in bronchiolar obstruction type. 5. The bronchospasm type was most frequently observed, followed by bronchospasm-hypersecretion type and bronchiolar obstruction type. 6. Spa therapy was effective in 74 cases (79.6%) out of the 93 patients, and regarding clinical classification of asthma type, spa therapy was most effective in the cases with bronchiolar obstruction type.
Keywords 気管支喘息 (Bronchial asthma) 温泉療法 (Spa therapy) 臨床病型 (Asthma type)
Publication Title 環境病態研報告
Published Date 1989-07
Volume volume60
Start Page 25
End Page 30
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309015
JaLCDOI 10.18926/19779
Title Alternative Spa therapy for bronchial asthma-Clinical effects of inhalation of iodine salt soluions.
FullText URL 060_019_024.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki|
Abstract 気管支喘息20例を対象に,ヨードゾル吸入療法を行ない,その臨床効果について検討を加えた。1.ヨードゾル2週間吸入後の臨床効果判定では,著効4例(20%),有効11例(75%),やや有効4例(20%),無効1例(5%)であり,有効以上の明らかな有効例は20例中15例(75%)であった。また臨床病型別の効果の検討では,明らかな有効例は,Ⅰa.気管支攣縮型では9例中6例(66.7%),Ⅰb.気管支攣縮+過分泌型では9例中7例(77.8%)であった。一方,Ⅱ.細気管支閉塞型では2例いずれも有効であった。2.ヨードゾル1回吸入後の換気機能の改善率は,FVC8.5%,FEV(1.0)11.0%,PEFR9.7%,MMF12.2%, V(50)14.4%, V(25)14.8%であり,閉塞性換気障害を示すパラメーターの改善率がやや高く,なかでも小さい気道の換気障害と関連の深いMMFやV(25)の改善率が高い傾向がみられた。
Abstract Alternative Clinical effects of inhalation therapy by iodine salt solutions were studied in 20 patients with bronchial asthma. 1. Inhalation therapy by iodine salt solutions was markedly effective in 4 cases (20%), moderately in 11 cases (55%), slightly in 4 cases (20%) and not effective in 1 case (5 % ). Number of the effective cases was 6 (66.7%) out of 9 cases with bronchospasm type (Ⅰa), and 7 (77.8%) out of 9 cases with bronchospasm+ hypersecretion type (Ⅰb), respectively. The inhalation therapy was also effective in all two cases with bronchiolar obstruction type (Ⅱ). 2. % Improvement of ventilatory function after inhalation of iodine salt solution was 8.5% in FVC, 11.0% in FEV(1.0), 9.7% in PEFR, 12.2% in MMF, 14.4% in V(50) and 14.8% in V(25). The % increase in ventilatory function was higher in the parameters such as MMF and V(25), representihg ventilatory disturbance of small airways.
Keywords 気管支喘息 (Bronchial asthma) ヨードゾル吸入 (Inhalation of iodine salt sotutlon) 温泉療法 (Spa therapy) 換気機能 (Ventilatory function)
Publication Title 環境病態研報告
Published Date 1989-07
Volume volume60
Start Page 19
End Page 24
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309118
JaLCDOI 10.18926/19777
Title Alternative Spa therapy for bronchial asthma. Effect of spa therapy on adrenal glands function.
FullText URL 060_014_018.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki|
Abstract 気管支喘息30症例を対象に,血中コーチゾール値を観察することにより,温泉療法の副腎皮質機能に及ぼす影響について検討を加えた。1.対象30症例の平均血中コーチゾール値は,5.64±4.95γ/dℓであった。このうち,ステロイド依存性喘息15例では,2.25±2.51γ/dℓ,ステロイド非使用の喘息15例では,9.02±4.47γ/dℓであり,ステロイド依存性喘息症例で有 意の低下傾向が見られた(P<0.001)。2.1-3カ月間の温泉療法前後での比較では,療法前の平均血中コーチゾール値は5.26±4.98γ/dℓ,後では9.51±5.26γ/dℓであり,温泉療法により有意の上昇がみられた(P<0.05)。3.1回の温泉浴前後の血中コーチゾール値の変動では,変動のみられない群と上昇傾向を示す群の2つの症例群が観察された。このうち,変動のみられない症例群は,もともと血中コーチゾール値が正常であるか,あるいは副腎皮質機能が疲弊してしまっているかのいずれかであった。
Abstract Alternative Effect of spa therapy on adrenai glands function was studied in 30 patients with bronchial asthma, by observing serum cortisol levels. 1. The mean of serum cortisol levels was 5.64±4.95γ/dℓ in all cases, 2.25±2.51γ/dℓ in 15 cases with steroid dependent asthma and 9.02±4.47γ/dℓ in 15 cases without steroid therapy. There was a significant difference between the two groups (P<0.001). 2. Serum cortisol levels were measured before and after spa therapy for 1 to 3 months. The mean of serum cortisol levels increased from 5.26±4.98γ/dℓ before spa therapy, to 9.51±5.26γ/dℓ after spa therapy, which was significantly higher than the former (P<0.05). 3. Changes of serum cortisol levels after hot spring bath fot 20 min were observed. Serum cortisol levels seased in one group, and did not change in the other groups after 20-min hot spring bath. In no change groups of serum cortisol levels, adrenocortical function was normal in one group, and highly damaged in the other group.
Keywords 気管支喘息 (Bronchial asthma) 血中コーチゾール (Serum cortisol level) 温泉浴 (Hot spring bath) 温泉療法 (Spa therapy)
Publication Title 環境病態研報告
Published Date 1989-07
Volume volume60
Start Page 14
End Page 18
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309101
JaLCDOI 10.18926/19776
Title Alternative Spa therapy for chronic respiratory disease- in subjects admitted in 1988.
FullText URL 060_006_013.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki|
Abstract 1988年1月より12月までの1年間に当院へ入院した慢性呼吸器疾患患者62例を対象に,背景因子,臨床的特徴および温泉療法の臨床効果について検討を加えた。1.対象62例のうちわけは,気管支喘息49例,瀰漫性汎細気管支炎4例,肺気腫4例,アレルギー性肉芽腫性血管炎3例,肺結核,気管支拡張症各1例であった。2.これら62症例のうち,温泉療法を受けた症例は41例(66.1%)であった。3.温泉療法を受けた症例の地域分布では,鳥取県からの入院症例32例では14例(43.8%)であり,同様に岡山県からの入院症例17例では15例(88.2%),その他の県からの入院症例13例では12例(92.3%)であった。4.温泉療法の臨床効果は,気管支喘息では33例中著効12例,有効15例,やや有効5例,無効1例で,明らかな有効例は27例(81.9%)であった。また温泉療法は瀰漫性汎細気管支炎,アレルギー性肉芽腫性血管炎などに対しても有効であった。
Abstract Alternative Backgrounds, immpnological characteristics and clinical effects of spa therapy were examined in patients with chronic respiratory disease admitted at Misasa Branch Hospital in 1988. 1. Sixty two patients with chronic respiratory disease comprised 49 patients with brohchial asthma, 4 with diffuse panbronchiolitis, 4 with pulmonary emphysema, 3 with allergic granulomatous angitis, each 1 with lung tuberculosis and with bronchiectasia. 2. Forty one patients (66.1%) out of the 62 cases had spa therapy. 3. Out of 32 patients coming from Tottori prefecture, 14 cases (43.8%) received spa therapy. On the other hand, spa therapy was carried out for 15 cases (88.2%) out of the 17 cases from Okayama prefecture, and for 12 cases (92.3%) out of the 13 cases from the other prefectures (long distant areas). 4. Spa therapy was effective in 27 cases (81.9%) out of the 33 patients with bronchial asthma. Spa therapy also effective for patients with diffuse panbronchiolitis, and allergic granulomatous angitis.
Keywords 気管支喘息 (Bronchial asthma) 瀰漫性汎細気管支炎 (Diffuse panbronchiolitis) アレルギー性肉芽腫性血管炎 (Allergic granulomatous angitis) 温泉療法 (Spa therapy)
Publication Title 環境病態研報告
Published Date 1989-07
Volume volume60
Start Page 6
End Page 13
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309166
Author Matsubara, Fumie| Sudo, Michiyasu| Tanizaki, Yoshiro|
Published Date 1988-08
Publication Title 環境病態研報告
Volume volume59
Content Type Data or Dataset
JaLCDOI 10.18926/19769
Title Alternative Allergic reactions for severe intractable asthma and spa therapy
FullText URL 059_062_067.pdf
Author Tanizaki, Yoshiro|
Abstract 予後良好と言われる気管支喘息が,どのような反応系により重症難治化傾向を取るのか,そして温泉療法がどのように作用するのかについて,若干の検討を加えた。まず関与する反応系は,その惹起相,反応相(液性因子相,細胞性因子相)の組合せにより,代表的な3つの反応系に分類することができる。1)IgE-肥満細胞(惹起相);ヒスタミン(ロイコトリエン)(液性因子相);リンパ球,好塩基球,好酸球(細胞性因子相),2)IgG-好中球;ロイコトリエン;好中球,リンパ球,マクロファージ,3)非IgE系反応;PAF;好酸球,3')非IgE系反応;ロイコトリエン;リンパ球。このような機序がもし存在するとすれば,1)の反応系の関与する喘息はアトピー型喘息,2)は中高年発症型喘息,3)は所謂内因性喘息,3')は中高年発症型喘息として把握される可能性が強い。温泉療法は,気道清浄化作用および副腎皮質機能改善作用により,これらの反応系の発現を抑制する。
Abstract Alternative Reactions for severe intractable asthma and actions of spa therapy on the reactions were discussed. Reactions participatng in onset mechanism of bronchial asthma were divided into three major groups. The first group comprises IgE-mast cell (eliciting phase), histamine (leukotrienes) (reactive phase; humoral phase), and lymphocytes-basophils-eosinophils (reactive phase; cellular phase). The second group comprises IgG-neutrophils, leukotrienes, and neutrophils-lymphocytes-macrophages. The third group is induced by non-IgE reactions, followed by PAF and eosinophils (with blood eosinophilia), and by leukotrienes and lymphocytes (without blood eosinophilia). If these reactions are really present and participate in onset mechanism of bronchial asthma, the first group could be observed in atopic asthma, the second one in late onset asthma, and the third one in so-called intrinsic asthma with blood eosinophilia and in late onset asthma without blood eosinophilia. Spa therapy has clearing actions of airway and improves function of adrenal glands in severe intractable asthma.
Keywords 気管支喘息 (Bronchial asthma) 難治性喘息 (lntractable asthma)  アレルギー反応 (Allergic reaction) 温泉療法 (Spa therapy)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 62
End Page 67
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309085
JaLCDOI 10.18926/19764
Title Alternative Clinical studies on steroid-dependent intractable asthma
FullText URL 059_047_053.pdf
Author Kitani, Hikaru| Araki, Hiroyuki| Sudo, Michiyasu| Tanizaki, Yoshiro| Tsuji, Mituaki| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract ステロイド依存性重症難治性喘息56例について免疫アレルギー学的検討を行った。その結果41才以降発症のLate Onset Asthmaが約半数を占め,血清IgE値が比較的低値であり,ハウスダストに対しての皮内反応,特異的IgE抗体価,ヒスタミン遊離はいずれも全般的に低く,カンジダに対しての皮内反応,特異的IgG抗体価はむしろ比較的高く,肺機能では末梢気道閉塞の傾向が窺われ,臨床分類では,気管支攣縮+過分泌型,および細気管支閉塞型が多数を占める等の特徴を有することが明らかにされた。
Abstract Alternative Clinical studies on steroid-dependent intractable asthma were carried out in 56 patients relating to immuno-allergic aspects. 1. In most half of the patients with steroid-dependent intractable asthma, age at onset of the disease was 41 years old or over (so-called "Late Onset Asthma"). 2. The frequency of positive skin reaction for house dust was relatively low. whereas that for Candida was relatively high. 3. The serum IgE concentrations of these patients were in general low. Many patients showed low serum IgE concentrations of less than 300IU/ml. The concentrations of specific IgE antibodies for house dust and Candida albicans were also low. 4. The concentrations of specific IgG antibodies for Candida albicans were high compared to those for house dust. 5. Basophil histamine release induced by house dust and Candida albicans was not so remarkable in majoriy of these patients, while anti-IgE-induced histamine release from basophils ranged widely. 6. In these patients, small airway obstruction was suggested in ventilatory function test. 7. In clinical classificatin of asthma, type Ⅰa; bronchospasm type was a few, while type Ⅰb; hypersecretion and type Ⅱ; bronchiolor obstruction type were dominant in the cases studied here.
Keywords ステロイド依存性喘息 (Steroid-dependent asthma) 血清IgE (Serum-IgE) 特異的IgE (Specific lgE) 特異的IgG (Specific IgG) 中高年発症型喘息 (Late onset asthma)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 47
End Page 53
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309074
JaLCDOI 10.18926/19762
Title Alternative Atopic asthma in patients of middle or advanced age
FullText URL 059_042_046.pdf
Author Araki, Hiroyuki| Kitani, Hikaru| Sudo, Michiyasu| Tanizaki, Yoshiro|
Abstract 中高年令(41才以上)のアトピー型喘息患者の特徴を明らかにするために,アトピー型喘息患者88例を0~20才,21~40才,41~60才,61才以上の各年令別に分け,年令,性別,発症年令,既往歴,家族歴,末梢血好酸球,血清IgE値,皮内反応の各項目について検討を加えた。結果は,中高年のアトピー型喘息患者は1)その年代の全喘息患者に占める割合は低く,2)男性が多く,3)平均発症年令は高く,4)その他のアレルギー性疾患の既往歴を持たない症例が比較的多く,5)皮内反応では,ハウスダスト陽性率が比較的低く,カンジダ,アスペルギルスの陽性率は比較的高い傾向にあった。6)家族歴,末梢血好酸球,血清IgE値では,明らかな傾向は認められなかった。
Abstract Alternative In order to elucidate the characteristics of atopic asthma in patients of middle or advanced age (over 41 years old), 88 atopic asthmatic patients were divided into 4 groups according to their ages, 0~20, 21~40, 41~60 and over 61 years old, and age, sex, age of onset, past history, family history, number of peripheral eosinophils, serum IgE and skin test were compared among these groups. The results were as follows: Atopic ashmatic patients of middle or advanced age were 1) less frequent in all asthmatic patients of same age group, 2) more frequent in males than in females, 3) higher in averaged age of onset, 4) more frequent in cases without other allergic past histories, 5) less frequently positive to house dust and more frequently positive to Candida and Aspergillus in skin test, 6) not different in family history, number of peripheral eosinophils and serum IgE compared to other groups.
Keywords アトピー (Atopy) 中高年令者 (patients of middle or advanced age) 喘息 (asthma)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 42
End Page 46
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309184
JaLCDOI 10.18926/19758
Title Alternative Separation of paripheral leukocytes by counterflow centrifugation elutriation
FullText URL 059_037_041.pdf
Author Sudo, Michiyasu| Matsubara, Fumie| Araki, Hiroyuki| Kitani, Hikaru| Tanizaki, Yoshiro|
Abstract JE-6B rotor(Beckman)を用いてcounterflow centrifugation elutriationによる末梢血白血球の分離を試み,若干の検討を加えた。1.flow rateが低い場合はリンパ球が大部分を占めたが,flow rateの増加とともに頻度は減少した。2. flow rateの増加とともに,好塩基球の頻度が増加し,9-10ml/minでは頻度のみでなく細胞数の増加も認められた。各fractionにおけるヒスタミン含有量も同様の傾向が認められた。3.好中球のpeakはflow rate 13ml/minであり以後減少を示した。4.最終段階で好酸球が増加傾向を示しChamber内で最も高値を示した。以上の結果よりcounterflow centrifugation elutriationによる末梢血白血球細胞分離は,in vitroにおける実験に有用な手段である可能性が示唆された。
Abstract Alternative Separation of peripheral leukocytes was carried out in 5 healthy subjects by counterflow centrifugation elutriation with JE-6B rotor. 1. Number of lymphocytes was predominant at low flow rate (4.5ml/min) of counterflow centrifugation elutriation. 2. As flow rate was increased (9-10ml/min), the purity and number of basophils, and histamine content in the fraction were gradually increased. 3. The purity and number of neutrophils were gradually increased, and the purity was reached the peak at 13ml/min. 4. The purity and number of eosinophils were gradually increased at the late stage of the centrifugation elutriation. The results obtained here might suggest that cell separation by counterflow centrifugation elutriation is more useful for experiments, especially allergy testing, in vitro.
Keywords 逆流負荷遠心法 (Counterflow centrifugation elutriation) JE-6Bローター (JE-6B rotor) 細胞分離 (Cell separation) 好塩基球 (Basophils)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 37
End Page 41
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309189
JaLCDOI 10.18926/19755
Title Alternative 気管支喘息および慢性気管支炎患者における抗ヒトIgEによる好塩基球からのヒスタミン遊離
FullText URL 059_031_036.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki| Tsuji, Mitsuaki| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract Histamine release from basophils induced by anti-IgE was studied in 8 patients with chronic bronchitis and 50 patients with bronchial asthma by analyzing doseresponse curves. As the result, there were no significant differences in maximum percent histamine release from basophils among three groups of healthy subjects (24.7± 14.2%), patients with chronic bronchitis (27.7±22.1%) and those with bronchial asthma (28.4±17.0%). In the patients with bronchial asthma, the maximum percent histamine release was higher in accordance with higher serum IgE levels, and low maximum percent release was observed in patients with intrinsic asthma (14.1±7.2%). Study of dose-response curves of anti-IgE-induced histamine release showed that a negative slope from E(2) to E(1) was observed in both healthy subjects and patients with chronic bronchitis. The majority of asthmatics with serum IgE levels of 501IU/ml or over showed a positive slope from E(2) to E(1).
Abstract Alternative 気管支喘息50例,慢性気管支炎8例を対象に,抗ヒトIgE添加時の好塩基球からのヒスタミン遊離を全血法により行ない,その臨床的評価について検討を加えた。抗ヒトIgE海底時のMax % histamine releaseの平均は,健康人24.7±14.2%,慢性気管支炎27.7±22.1%,気管支喘息28.4±17.0%であり,3者間に有意の差はみられなかった。すなわち,抗ヒトIgE添加により健康人や慢性気管支炎患者の好塩基球からも有意のヒスタミン遊離が見られた。気管支喘息のなかでは,内因性喘息症例においてヒスタミン遊離(14.1±7.2%)の低い傾向が見られた。Dose-response curveの検討では,健康人,慢性気管支炎症例では全例E(2)からE(1)へかけてのnegative slopeを示した。気管支喘息症例では,血清IgE値500IU/ml以下の症例ではnegative slopeを示す症例が多く,一方501IU/ml以上の症例ではpositive slopeを示す症例がより多く見られた。
Keywords Basophil response (好塩基球の反応性) Anti-lgE (抗ヒトIgE) Histamine release (ヒスタミン遊離) chronic bronchitis (慢性気管支炎) bronchial asthma (気管支喘息)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 31
End Page 36
ISSN 09133771
language 英語
File Version publisher
NAID 120002309114
JaLCDOI 10.18926/19745
Title Alternative Maternal swimming at Misasa Hospital Of Okayama University Medical School First report. Recognition of the pregnant women to maternity swimming
FullText URL 059_008_011.pdf
Author Okuda, Hiroyuki| Suga, Kiyohiro| Sakata, Junko| Yosida, Sachie| Mifune, Masaaki| Tanizaki, Yoshiro|
Abstract 妊婦のスポーツに対する考え方は近年まで比較的消極的であったが,最近マスコミに取り上げられるようになり,妊婦水泳・エアロビクスダンス等は全国的にもかなり普及していると思われる。今回外来通院患者を対象に妊婦水泳に関するアンケート調査を行った結果,大多数のものが関心を持ち,また実施希望を持っていることが判明したのでその概要を報告する。
Abstract Alternative The sports have been contraindication for pregnant women till resent years. Since Dr. Murooka reported the safety and the effectiveness of maternity swimming in 1978. maternity swimming have been taken up and fasionarised by mass media. And then maternity swimming becomes popular in our all over the country. Now we made inquiries about maternity swimming to 25 pregnant women in our outpatients clinic. (Results) 1. Eightyfour percent of them were interested in maternity swimming. 2. Eightyeight percent of them wanted to swim. 3. Most of them had little knowledge about tocological effectiveness of maternity swimming. (Conclusion) It is nessesary that the enlightenment and the active management of maternity swimming by obstetricians.
Keywords 妊婦水泳 (Maternity swimming)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 8
End Page 11
ISSN 09133771
language 日本語
File Version publisher
NAID 120002308990
JaLCDOI 10.18926/19743
Title Alternative Spe therapy for chronic respiratory disease-in subjects admitted in 1987.
FullText URL 059_001_007.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki|
Abstract 1987年1月より12月までの1年間に当院へ入院した慢性呼吸器疾患患者52例を対象に,その背景因子,免疫アレルギー学的要素および温泉療法の臨床効果について検討を加えた。1.対象52例のうちわけは,気管支喘息37例,瀰漫性汎細気管支炎3例,慢性咳嗽3例,アレルギー性肉芽腫性血管炎2例,慢性気管支炎2例,過敏性肺臓炎2例,気管支拡張症,肺気腫,広汎性肺結核症各1例であった。2.これら52症例のうち,温泉療法を受けた症例は36例(69.2%)であった。3.対象症例の地域分布と温泉療法を受けた症例との関係では,鳥取県内の入院症例(26例)で温泉療法を受けた症例は11例(42.3%)であり,県外(遠隔地)からの入院症例(26例)で温泉療法を受けた症例は25例(96.2%)であった。4.温泉療法の臨床効果では,気管支喘息では30例中25例(83.3%)で有効であり,その他1瀰漫性汎細気管支炎,アレルギー性肉芽腫性血管炎,気管支拡張症などで有効であった。
Abstract Alternative Backgrounds, immunoallergological characteristics and clinical effects of apa therapy were examined in patients with chronic respiratory diseases admitted at Misasa Branch Hospital in 1987. 1. Fifty five patients with chronic respiratory diseases comprised 37 patients with bronchial asthma, 3 with diffuse panbronchiolitis, 3 with chronic cough, 2 with allergic granulomatous angitis, 2 with chronic bronchitis, 2 with hypersensitivity pneumonitis, each 1 with bronchiectasia, pulmonary emphysema and lung tuberculosis. 2. Thirty six patients (69.2%) out of the 52 cases had spa therapy. 3. Out of 22 patients coming from Tottori prefecture, 11 cases (42.3%) received spa therapy. On the other hand, spa therapy was carried out for 25 cases (96.2%) out of the 26 cases coming from the other prefectures (long distant areas). 4. Spa therapy was effective in 25 cases (83.3%) out of the 30 patients with bronchial asthma. Spa therapy was also effective for patients with diffuse panbronchiolitis, allergic granulomatous angitis and bronchiectasia.
Keywords 気管支喘息 (Bronchial asthma) 瀰漫性汎細気管支炎 (Diffuse panbronchiolitis) 気管支炎 気管支肺胞洗浄法 (Bronchoalveolar lavage) 温泉療法 (Spa therapy)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 1
End Page 7
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309207
Author Matsubara, Fumie| Sudo, Michiyasu| Tanizaki, Yoshiro| Morinaga, Hiroshi|
Published Date 1987-08
Publication Title 環境病態研報告
Volume volume58
Content Type Data or Dataset
JaLCDOI 10.18926/19735
Title Alternative Spa therapy for bronchial asthma 2. Clinical and fundamental evaluation procedures
FullText URL 058_035_039.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu|
Abstract 気管支喘息に対する温泉療法の効果が,これまでどのような評価方法により判定されてきているのかについて,若干の文献的考察を加えた。効果に対する評価方法は,臨床的評価と作用機序と関連した評価方法とに分けて考えることができる。臨床的評価方法としては,自,他覚症状の改善,同時に使用された薬剤の減量の程度,あるいは,肺機能検査の各パラメーターの変動の比較などの方法により行なわれている。一方作用機序と関連した評価では,喘息の発症機序とある程度関連した温泉療法の作用を中心に検討が加えられている。例えば,喘息の発症機序として重要な位置を占める免疫アレルギー反応,自立神経系,内分泌系などと関連した指標を用いて,その変動を観察することによって,効果を明らかにしようとする試みがなされている。これらの検討は極めて意義深いが,なお今後IgE系反応や気道の過敏性との関連 などについての追求が必要であるように考えられる。
Abstract Alternative Evaluation procedures for effectiveness of spa therapy on bronchial asthma were discussed according to the reports published. The evaluation procedures could be divided into tow groups : procedures by clinical observations and procedures by findings relating to action mechanisms of spa therapy. Clinical evaluations have been performed by subjective and objective symptoms, the degree of reduction of drugs used for treatment of bronchial asthma, and improvement of each parameter in ventilatory function test. The results obtained by these procedures are frequently reported as clinical observations. On the other hand, evaluations based on the findings relating to action mechanisms of spa therapy have been carried out as follows : 1) observations for immuno-allergic reactions which play an important role in pathogenic mechanisms of bronchial asthma. 2) observations for autonomic nerve system, and 3) for endocrine glands, which have a close correlation with onset mechanisms of bronchial asthma. The results obtained by these findings before and after spa therapy are much interesting, and should be investigated furthermore.
Keywords 温泉療法 (spa therapy) 気管支喘息 (bronchial asthrna) 免疫アレルギー反応 (immuno-allergic reaction) 自律神経系 (autonomic nerve) 内分泌系 (endocrine)
Publication Title 環境病態研報告
Published Date 1987-08
Volume volume58
Start Page 35
End Page 39
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309078
JaLCDOI 10.18926/19731
Title Alternative Spa therapy for bronchial asthma 1. Climatotherapy, its clinical effects
FullText URL 058_031_034.pdf
Author Tanizaki, Yoshiro| Sudo, Michiyasu|
Abstract 気管支喘息に対する温泉療法,特に温泉所在地(保養地)の環境,すなわち気候条件の喘息に対する影響について,文献的考察を加えた。気候療法としては,主として山岳気候,海洋性気候,洞窟気候などが,喘息の治療に応用されている。これらの気候は,いずれも喘息に対して有効的に作用することが示されているが,それぞれの特徴を持っている。例えば,山岳気候は酸素分圧が低いこと,水蒸気圧が低いことなどが特徴であり,そのため生体がそれに適合するためにはある程度の時間が必要となる。一方海洋性気候は,海塩粒子を含んでおり,海水エロゾル中のカルシウムやマグネシウムなどが良い影響を与えると考えられている。また洞窟気候は,水分含量が均一であり,電解質,特に陰イオンが多く,これが粘膜活性や浄化活性を亢進させると言われている。またいずれの気候療法も,副腎皮質機能の活性化をもたらすと考えられている。
Abstract Alternative Spa therapy includes influence of environmental factors, especially climate around the place at which spa therapy is performed. In the present article, the effects of environmental climate around the hot springs on bronchial asthma were discussed according to the reports published. Mountain climate, sea climate and underground climate are reported to be effective for the therapy of bronchial asthma. Although these climates have shown remarkable effectiveness on bronchial asthma, each of them has climatic characteristics in treatment for bronchial asthma. Mountain climate has low content of oxygen and low vapor pressure, and patients with bronchial asthma require several weeks to adapt themselves for the climate. Sea climate is rich in cations and anions, and Ca(2+) and Mg(2+) included in sea water aerosol act beneficially on mucous membrane of airways in patiepts with bronchial asthma. Underground climate includes a lot of anions, which activate the activity of mucous membrane and ability of clearance of airways. Each climate also activate the function of adrenal glands.
Keywords 気候療法 (climatotherapy) 気管支喘息 (bronchial asthrna) 山岳気候 (mountain climate) 海洋性気候 (sea climate) 洞窟気候 (underground climate)
Publication Title 環境病態研報告
Published Date 1987-08
Volume volume58
Start Page 31
End Page 34
ISSN 09133771
language 日本語
File Version publisher
NAID 120002308993
JaLCDOI 10.18926/19710
Title Alternative Study on anti-allergic action of Terfenadine
FullText URL 058_005_011.pdf
Author Tanizaki, Yoshiro| Komagoe, Haruki| Sudo, Michiyasu| Kitani, Hikaru|
Abstract 選択的H(1)受容体拮抗薬であるTerfenadineの抗アレルギー作用,特にIgE系反応によるヒスタミン,およびCa ionophore A23187による好中球からのleukotrienes遊離に対する抑制作用を,ヒト末梢血白血球を用いて検討した。1.Terfenadineは,抗ヒトIgE刺激による健康人好塩基球および特異抗原刺激による気管支喘息患者好塩基球からのヒスタミン遊離に対して,全血法でのin vitro添加実験では有意の抑制作用を示さなかった。 2.洗浄白血球法によるin vitro添加実験では,Terfenadineは特異抗原および抗ヒトIgE刺激時の気管支喘息患者好塩基球からのヒスタミン,Ca ionophore A23187刺激時のleukotriene B(4)遊離に対して有意の抑制効果を示した。3.Terfenadineの内服によるin vivoの抑制実験では,IgE系反応による健康人および気管支喘息患者好塩基球からのヒスタミン遊離に対して有意の抑制効果は見られなかったが,特異抗原による皮膚反応に対しては明らかな抑制作用が観察された。以上より,Terfenadineは,組織肥満細胞および組織へと遊走してきた好塩基球に対してはある程度の抑制作用を有しているものと考えられた。
Abstract Alternative Anti-allergic action of terfenadine, one of the H(1) receptor inhibitors, was examined by human basophil histamine release induced by anti-IgE or a specific allergen (house dust), and the release of leukotriens from leucocytes stimulated by Ca ionophore A23187. 1. Terfenadine did not inhibit the release of histamine from basophils of healthy subjects induced by anti-IgE, and also of patients with bronchial asthma induced by house dust when the in vitro examination was performed by a whole blood method. 2. Histamine release from basophils of patients with bronchial asthma was significantly inhibited when washed leucocytes was preincubated with terfenadine in vitro. The release of LT B(4) from leucocytes was also inhibited. 3. In vivo examination of inhibitory action of terfenadine showed no effect on basophil histamine release of healthy subjects by anti-IgE and of asthmatic subjects by house dust, although skin test by house dust in these subjects was inhibited 60 min after administration in vivo. These results show that terfenadine inhibits the release of chemical mediators from tissue mast cells, and basophils migrating into local allergic sites.
Keywords 夕一フェナンジン (Terfenadine) lgE系反応 (IgE-mediated reaction) ヒスタミン (histamine) 好塩基球 (basophils)
Publication Title 環境病態研報告
Published Date 1987-08
Volume volume58
Start Page 5
End Page 11
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309197
Author Inoue, Taeko| Tanizaki, Yoshiro| Morinaga, Hiroshi|
Published Date 1986-07
Publication Title 環境病態研報告
Volume volume57
Content Type Data or Dataset
JaLCDOI 10.18926/19683
Title Alternative Studies on anti-allergic actions of tranilast (Rizaben®)
FullText URL 057_042_045.pdf
Author Tanizaki, Yoshiro| Komagoe, Haruki| Sudo, Michiyasu| Morinaga, Hiroshi| Ohtani, Jun| Kimura, Ikuro|
Abstract 抗アレルギー剤(脱顆粒抑制剤)の1つであるtranilastの肥満細胞の遊離機序に対する抑制作用について検討を加えた。1.抗原刺激時の肥満細胞の(45)Ca uptakeおよびヒスタミン遊離に対して,tranilastは有意の抑制作用を示したが,comp.48/80刺激時にはtranilastの(45)Ca uptake,ヒスタミン遊離に対する抑制作用はほとんどみられなかった。2.phosphatidylserine添加時には,(45)Ca uptakeに対するtranilastの抑制作用は減弱傾向を示し,この傾向はヒスタミン遊離に対する作用に比べより高度であった。3.抗原および抗ヒトIgEによる好塩基球からのヒスタミン遊離に対して,tranilastは明らかな抑制作用を示さなかった。
Abstract Alternative Tlanilast is clinically used for bronchial asthma as one of the prophylactic agents for asthma attacks. In this study, anti-allergic actions of tranilast were examined in (45)Ca uptake of and histamine release from target cells of IgE after stimulation with antigen, anti-IgE and camp. 48/80. 1. (45)Ca uptake of and histamine release from rat peritoneal mast cells stimulated by antigen were significantly inhibited by preincubation of the cells with tranilast. Inhibition by tranilast of increased (45)Ca uptake of mast cells by addition of phosphatidylserine were less, although inhibition of histamine release was not so affected by addition of phosphatidylserine. No significant inhibition by tranilast of (45)Ca uptake and histamine release was not observed when the cells were stimulated by camp. 48/80. 2. Tranilast showed any inhibitory effects on basophil histamine release induced by antigen and anti-IgE.
Keywords トラニラスト (tranilast) (45)Ca取り込み ((45)Ca uptake) ヒスタミン遊離 (histamine release) 肥満細胞 (mast cell) 好塩基球 (basophils)
Publication Title 環境病態研報告
Published Date 1986-07
Volume volume57
Start Page 42
End Page 45
ISSN 09133771
language 日本語
File Version publisher
NAID 120002308998