JaLCDOI 10.18926/AMO/31330
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Author Matsuo, Toshihiko| Tsutsui, Yasuko| Matsuo, Nobuhiko|
Abstract <p>To test transdifferentiation of retinal pigment epithelial (RPE) cells in suspension culture, chick embryonic RPE sheets and dissociated RPE cells were cultured for two months in a non-adherent dish for suspension culture. RPE cells, isolated as a sheet, aggregated immediately and remained the same size with their differentiated characteristics for two months. The presence of basic fibroblast growth factor (bFGF) at concentrations of 10 ng/ml or higher induced the formation of a spherical lentoid structure which was positive for crystallin and bFGF receptor. In contrast, dissociated RPE cells did reaggregate but did not develop the lentoid structure even in the presence of bFGF. The transdifferentiation of RPE cells to the lentoid structure in this study was in contrast to their transdifferentiation to the retina, as reported in a previous study.</p>
Keywords basic fibroblast growth factor multicellular spheroids retinal pigment epithelium transdifferentiation lens lentoid
Amo Type Article
Published Date 1998-06
Publication Title Acta Medica Okayama
Volume volume52
Issue issue3
Publisher Okayama University Medical School
Start Page 125
End Page 130
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9661738
Web of Sience KeyUT 000074528500001