このエントリーをはてなブックマークに追加
ID 32195
JaLCDOI
FullText URL
Author
Hongo, Toshiki
Tomoda, Jun
Mizuno, Motowo
Maga, Toshirou
Tsuji, Takao
Abstract
Mucus glycoprotein is one of the major components of gastric mucus which plays an important role in mucosal defensive mechanisms as a mucus-bicarbonate barrier. Analysis of the mucus glycoprotein synthesis is a useful tool for evaluating gastric mucosal defensive factors. UDP-galactosyltransferase (UDP-Gal-T) is one of the regulating enzymes for the synthesis of the mucus glycoprotein. In the present paper, we studied assay methods for UDP-Gal-T activity in rat gastric mucosa using radiolabeled UDP-galactose and two different kinds of acceptor proteins, namely ovomucoid and asialomucin, and analyzed effects of antisecretory agents on the UDP-Gal-T activity. We used crude supernatants of homogenized scrapings of the fundic part of rat stomach as an enzyme preparation and determined optimal conditions. In each acceptor, Mn2+ and the non-ionic detergent Triton X-100 were required for the enzyme activity. With each acceptor molecule, the type of glycosidic linkages of galactose was beta-type linkage. With asialomucin as an acceptor, UDP-Gal-T activities of rat gastric mucosa decreased after intraperitoneal administration of antisecretory agents, while change of the enzyme activity was not observed with ovomucoid as an acceptor.
Keywords
rat gastric mucosa
UDP-galactosyltransferase
ovomucoid
asialomucin
Amo Type
Article
Published Date
1991-10
Publication Title
Acta Medica Okayama
Volume
volume45
Issue
issue5
Publisher
Okayama University Medical School
Start Page
301
End Page
308
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
Copyright Holders
Copyright© 1999 Okayama University Medical School
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/6217