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ID 55867
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Sawada, Takahiro Graduate School of Environmental and Life ScienceOkayama University
Eguchi, Miho Graduate School of Environmental and Life ScienceOkayama University
Asaki, Seiya Faculty of AgricultureOkayama University
Kashiwagi, Ryota Faculty of AgricultureOkayama University
Shimomura, Kousuke Faculty of AgricultureOkayama University
Taguchi, Fumiko Graduate School of Environmental and Life ScienceOkayama University
Matsui, Hidenori Graduate School of Environmental and Life ScienceOkayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life ScienceOkayama University
Noutoshi, Yoshiteru Graduate School of Environmental and Life ScienceOkayama University
Toyoda, Kazuhiro Graduate School of Environmental and Life ScienceOkayama University
Ichinose, Yuki Graduate School of Environmental and Life ScienceOkayama University
Abstract
Our previous studies revealed that flagellar-motility-defective mutants such as ∆fliC of Pseudomonas syringae pv. tabaci 6605 (Pta6605) have remarkably reduced production of N-acyl-homoserine lactones (AHL), quorum-sensing molecules. To investigate the reason of loss of AHL production in ∆fliC mutant, we carried out transposon mutagenesis. Among approximately 14,000 transconjugants, we found 11 AHL production-recovered (APR) strains. In these APR strains, a transposon was inserted into either mexE or mexF, genes encoding for the multidrug efflux pump transporter MexEF-OprN, and mexT, a gene encoding a putative transcriptional activator for mexEF-oprN. These results suggest that MexEF-OprN is a negative regulator of AHL production. To confirm the negative effect of MexEF-OprN on AHL production, loss- and gain-of-function experiments for mexEF-oprN were carried out. The ∆fliC∆mexF and ∆fliC∆mexT double mutant strains recovered AHL production, whereas the mexT overexpressing strain abolished AHL production, although the psyI, a gene encoding AHL synthase, is transcribed as wild type. Introduction of a mexF or mexT mutation into another flagellar-motility- and AHL production-defective mutant strain, ∆motCD, also recovered the ability to produce AHL. Furthermore, introduction of the mexF mutation into other AHL production-defective mutant strains such as ∆gacA and ∆aefR also recovered AHL production but not to the ∆psyI mutant. These results indicate that MexEF-OprN is a decisive negative determinant of AHL production and accumulation.
Keywords
Flagella motility
MexEF-OprN
Multidrug efflux pump transporter
N-Acyl-homoserine lactone
Quorum sensing
Note
This is an Accepted Manuscript of an article published by Springer Nature 2018
Published Date
2018-03-16
Publication Title
Molecular Genetics and Genomics
Volume
volume293
Issue
issue4
Publisher
Springer
Start Page
1
End Page
11
ISSN
16174615
NCID
AA11547886
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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isVersionOf https://doi.org/10.1007/s00438-018-1430-9