Author Okada, Soji| Kawanishi, Koichi| Ofuji, Tadashi|
Published Date 1975-06
Publication Title Acta Medica Okayama
Volume volume29
Issue issue3
Content Type Journal Article
Author Hiraki, Kiyoshi| Ofuji, Tadashi| Watari, Zenji|
Published Date 1956-07
Publication Title Acta Medicinae Okayama
Volume volume10
Issue issue3
Content Type Journal Article
Author Hiraki, Kiyoshi| Ofuji, Tadashi| Hattori, Yoshiyuki|
Published Date 1956-07
Publication Title Acta Medicinae Okayama
Volume volume10
Issue issue3
Content Type Journal Article
Author Hiraki, Kiyoshi| Ofuji, Tadashi| Sunami, Hiroshi|
Published Date 1956-07
Publication Title Acta Medicinae Okayama
Volume volume10
Issue issue3
Content Type Journal Article
JaLCDOI 10.18926/AMO/30561
FullText URL fulltext.pdf
Author Miyoshi, Masanori| Nakashima, Yusaku| Hirakawa, Shuzo| Mitsunaga, Mikio| Nakagawa, Osamu| Miura, Hiroshi| Suzuki, Shinya| Ofuji, Tadashi|
Abstract <p>Function of pituitary-thyroid axis was studied in rats with experimentally induced thyroiditisWistar strain female rats were immunized with homologous thyroid extract in Freund's complete adjuvant and with simultaneous intradermal injection of pertussis vaccine concentrate. They received booster shots at the first and third week after the initial immunization. Serum thyroid hormones and TSH were measured just before, and at weekly intervals after, the initial immunization. Histological examination of the thyroid gland at the second week after immunization showed slight infiltration of macrophages in the thyroid follicles. From the third to the fourth week, massive lymphoid cell infiltration and destruction of follicular architecture developed in all immunized rats. Serum R3 levels slightly decreased during the second week, increased transiently during the third week, then decreased again thereafter. Serum T4 levels decreased slightly durinf the fourth week. Serum TSH levels were not elevated significantly during the third week, but the response to TRH was significantly increased at this time. Basal TSH levels were increased during the fourth week. The TRH test was a sensitive method capable of detecting minimal failure of thyroid function undetected by other routine measuremens of thyroid hormones and TSH.</p>
Keywords thyroid function thyrotropin (TSH) experimental thyroiditis thyroid hormones.
Amo Type Article
Published Date 1980-02
Publication Title Acta Medica Okayama
Volume volume34
Issue issue1
Publisher Okayama University Medical School
Start Page 11
End Page 17
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6446835
Web of Science KeyUT A1980JS13800002
JaLCDOI 10.18926/AMO/30562
FullText URL fulltext.pdf
Author Takaya, Yasumasa| Ota, Zensuke| Makino, Hirofumi| Kida, Keiko| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
Abstract Isolated and purified glomerular basement membranes (GBM) of normal and aminonucleoside (PAN) nephrosis rats were observed by electron microscopy after negative staining. Although GBM of normal rats appeared as a molecular sieve with uniform pores, GBM of nephrotic rats showed enlargement and elongation of the pores. For an average of fifty pores, the long dimension was 40.4+/-10.7 A and the short dimension 13.8+/-3.6 A in nephrosis whereas the long dimension was 12.3+/-2.5 A and the short dimension 8.4+/-1.0 A in normal rats. Changes in the pores in GBM were thought to result in increased permeability of serum protein and hence proteinuria.
Keywords gromerular basement membranc aminonucleoside nephrosis negative staining.
Amo Type Brief Note
Published Date 1980-02
Publication Title Acta Medica Okayama
Volume volume34
Issue issue1
Publisher Okayama University Medical School
Start Page 67
End Page 70
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6446842
Web of Science KeyUT A1980JS13800009
JaLCDOI 10.18926/AMO/30709
FullText URL fulltext.pdf
Author Okada, Soji| Miyai, Yooichiro| Ofuji, Tadashi|
Abstract <p>The patient was a 37-year-old female teacher with hyperemesis diabeticorum and juvenile Type-I diabetes. At the age of 29 years, nausea and vomiting developed and secured at nearly weekly intervals. She was started on clotiazepam (15 mg/day). The vomiting was cured and psychological improvement was evident; her anxiety about diabetes was markedly reduced. An X-ray examination after the administration of clotiazepam showed that she was entirely free from marked hypoperistalsis and the severe retention of gastric contents which had been present before this treatment. The present case is a clear example of stress closely related to the pathogenesis of hyperemesis diabeticorum.</p>
Keywords antianxiety drug hyperemesis diabeticorum
Amo Type Brief Note
Published Date 1982-02
Publication Title Acta Medica Okayama
Volume volume36
Issue issue1
Publisher Okayama University Medical School
Start Page 81
End Page 84
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6121462
Web of Science KeyUT A1982NE20000010
Author Ota, Zensuke| Makino, Hirofumi| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
Published Date 1977-10
Publication Title Acta Medica Okayama
Volume volume31
Issue issue5
Content Type Journal Article
Author Kawanishi, Koichi| Ueda, Hidenori| Nagase, Masayuki| Ofuji, Tadashi|
Published Date 1977-10
Publication Title Acta Medica Okayama
Volume volume31
Issue issue5
Content Type Journal Article
Author Okada, Soji| Yagyu, Fumito| Kawanishi, Koichi| Ofuji, Tadashi|
Published Date 1977-06
Publication Title Acta Medica Okayama
Volume volume31
Issue issue3
Content Type Journal Article
Author Ogawa, Norio| Miyoshi, Masanori| Takahara, Jiro| Ofuji, Tadashi|
Published Date 1975-10
Publication Title Acta Medica Okayama
Volume volume29
Issue issue5
Content Type Journal Article
Author Kawanishi, Koichi| Goto, Akio| Ishida, Toshihiko| Kawamura, Ko| Nishina, Yoshiaki| Machida, Shuji| Yamamoto, Shinro| Ofuji, Tadashi|
Published Date 1978-08
Publication Title Acta Medica Okayama
Volume volume32
Issue issue4
Content Type Journal Article
Author Sarai, Tetsuo| Kawanishi, Koichi| Saito, Yoshihito| Aoi, Katsuyuki| Nishina, Yoshiaki| Ofuji, Tadashi|
Published Date 1978-08
Publication Title Acta Medica Okayama
Volume volume32
Issue issue4
Content Type Journal Article
Author Yamana, Seizo| Matsueda, Hideki| Ezawa, Hidemitsu| Miyake, Susumu| Katsuyama, Shunsaku| Ofuji, Tadashi|
Published Date 1978-12
Publication Title Acta Medica Okayama
Volume volume32
Issue issue6
Content Type Journal Article
Author Ofuji, Tadashi| Yamachika, Yukio| Fukuda, Genjiro| Kamura, Junta| Kotani, Saburo| Ota, Zensuke| Kitajima, Koichi|
Published Date 1959-06
Publication Title Acta Medicinae Okayama
Volume volume13
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/31274
FullText URL fulltext.pdf
Author Makino, Hirofumi| Takaoka, Michio| Komoda, Keizo| Tokuyama, Kiyoyuki| Ota, Zensuke| Ofuji, Tadashi| Kaneshige, Tetsuji|
Abstract <p>Masugi nephritis was induced in rats by a single intravenous injection of rabbit anti-rat kidney serum, and studied with a scanning electron microscope. A characteristic finding was the presence of white cells, probably polymorphonuclear leukocytes, with many microspikes which penetrated through degenerated glomerular endothelial cells to be in direct contact with the glomerular basement membrane. This finding confirms the pathogenic role of leukocytes in glomerulonephritis induced by anti-glomerular basement membrane antibody.</p>
Keywords Masugi nephritis scanning electron microscopy leukocyte.
Amo Type Brief Note
Published Date 1981-11
Publication Title Acta Medica Okayama
Volume volume35
Issue issue5
Publisher Okayama University Medical School
Start Page 373
End Page 376
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6459000
Web of Science KeyUT A1981MS42400007
JaLCDOI 10.18926/AMO/31275
FullText URL fulltext.pdf
Author Asano, Taro| Yano, Keisuke| Ofuji, Tadashi|
Abstract <p>To investigate cellular interactions between human T and B lymphocytes in various diseases, we established a technique to prove terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. We also established a double antibody radioimmunoassay to measure the amount of IgG, IgA and IgM synthesized and secreted in culture supernatants. Purified immunoglobulins were obtained from sera of patients with myeloma or macroglobulinemia. The peripheral blood lymphocytes from 25 normal individuals had the geometric mean synthetic rates of 1886 ng for IgG, 1607 ng for IgA and 1173 ng for IgM per 1 X 10(6) cells when cultured for nine days in the presence of pokeweed mitogen. The method is simple and sensitive, and is thought to be useful for examining human lymphocyte function in vitro.</p>
Keywords lymphocytes PWM immunoglobulins RIA.
Amo Type Article
Published Date 1981-11
Publication Title Acta Medica Okayama
Volume volume35
Issue issue5
Publisher Okayama University Medical School
Start Page 319
End Page 326
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6458995
Web of Science KeyUT A1981MS42400002
JaLCDOI 10.18926/AMO/31290
FullText URL fulltext.pdf
Author Hashimoto, Kozo| Ohno, Norihito| Aoki, Yoshiyuki| Kageyama, Jingo| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>The distribution of corticotropin releasing factor (CRF) and arginine vasopressin (AVP) in hypothalamic nuclei were examined in control and estrogen-treated female rats. CRF activity was measured using monolayer cultured rat anterior pituitary cells and AVP by radioimmunoassay. Hypothalamic nuclei were punched out according to the method of Palkovits. The distribution of CRF activity in 5 different hypothalamic nuclei was similar to that of AVP in intact female rats. CRF activity in hypothalamic nuclei, pituitary ACTH content and plasma ACTH levels in estrogen-treated rats were not significantly different from those in control rats. However, significant elevation of AVP content was observed in the supraoptic and paraventricular nuclei of estrogen-treated rats. These results indicate that CRF and AVP are distributed in similar hypothalamic nuclei, but that they are not identical.</p>
Keywords corticotropin releasing factor (CRF) arginine vasopressin (AVP) hypothalamic nucler.
Amo Type Article
Published Date 1981-02
Publication Title Acta Medica Okayama
Volume volume35
Issue issue1
Publisher Okayama University Medical School
Start Page 37
End Page 43
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6266205
Web of Science KeyUT A1981LH76300004
JaLCDOI 10.18926/AMO/31291
FullText URL fulltext.pdf
Author Ohno, Norihito| Hashimoto, Kozo| Yunoki, Sho| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>Chromosome studies were conducted on two patients with adult T-cell leukemia. In both patients, a marker chromosome 14q+ and a structural change involving chromosome 1 with trisomy of the q arm were found in peripheral blood leukocytes. The 14q+ marker chromosome had resulted from translocation from #5p in one patient and #5q in the other patient. The present and previous studies suggest that the donor chromosomes involved in the 14q+ translocation are variable. This indicates that the 14q+ marker chromosome rather than the donor chromosome is intimately related with adult T-cell leukemia.</p>
Amo Type Article
Published Date 1981-02
Publication Title Acta Medica Okayama
Volume volume35
Issue issue1
Publisher Okayama University Medical School
Start Page 19
End Page 25
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6455044
Web of Science KeyUT A1981LH76300002
JaLCDOI 10.18926/AMO/31292
FullText URL fulltext.pdf
Author Ohno, Norihito| Hashimoto, Kozo| Yunoki, Sho| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>A perifusion method has been developed using rat hypothalamo-neurohypophyseal system (HNS) or neural lobe to investigate the control mechanism of arginine vasopressin (AVP) release. A specific radioimmunoassay (RIA) for AVP was developed to measure AVP in perifusion medium employing anti-AVP serum which was obtained by immunizing rabbits. At a final dilution of 1/12,000, the antiserum showed less than 0.66 and 0.01% cross reactivity with lysine-vasopressin and oxytocin, respectively. But it did not cross reacted with other peptide hormones. The lowest detectable level of vasopressin was 0.5 pg/tube. The intra-assay coefficient of variation averaged 10.4%. The dilution curve of perifused medium was well paralled to the standard curve of AVP assay. AVP release from HNS or neural lobe gradually declined to the stable level in 90-120 min after the initiation of perifusion. Good repeatability of the AVP release from neural lobe was recognized by repeated stimulation with 10 min perifusion of 60 mM KCl at every 60 min. HNS released AVP in dose related manner to the osmotic challenge of sodium or glucose, and AVP release was stimulated from HNS by prostaglandin E2, but not by dopamine. These results show that the perifusion methods using AVP-RIA is a useful method to examine the AVP release from HNS or neural lobe.</p>
Amo Type Article
Published Date 1981-02
Publication Title Acta Medica Okayama
Volume volume35
Issue issue1
Publisher Okayama University Medical School
Start Page 27
End Page 35
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6455045
Web of Science KeyUT A1981LH76300003