Author Yamamoto, Michio| Tanimoto, Junichi| Kojima, Sumikazu| Nobuki, Shigeo| Hagitani, Akira| Iguchi, Yoshiko| Akagi, Eiko| Ofuji, Tadashi| Asaka, Takakazu| Kamura, Junta| Shibata, Tsuneo|
Published Date 1960-04
Publication Title Acta Medicinae Okayama
Volume volume14
Issue issue1
Content Type Journal Article
Author Hiraki, Kiyoshi| Ofuji, Tadashi| Watari, Zenji|
Published Date 1956-07
Publication Title Acta Medicinae Okayama
Volume volume10
Issue issue3
Content Type Journal Article
JaLCDOI 10.18926/AMO/31042
FullText URL fulltext.pdf
Author Kawanishi, Koichi| Goto, Akio| Ishida, Toshihiko| Kawamura, Ko| Nishina, Yoshiaki| Machida, Shuji| Yamamoto, Shinro| Ofuji, Tadashi|
Abstract <p>Effects of synthetic neurotensin on the endocrine pancreas were studied in nine normal and six hypophysectomized (10th to 14th day post-hypophysectomy) dogs. Synthetic neurotensin was administered into the superior pancreaticoduodenal artery, and plasma insulin and glucagon concentrations were measured radioimmunologically. In normal dogs, ten microgram/kg neurotensin administration brought about a mild hyperglycemic response and sharp and rapid increase of plasma insulin and glucagon concentrations in the superior pancreaticoduodenal vein. A biphasic insulin response was noted in the pancreatic vein. The results suggest that a large dose of neurotensin acts directly on the endocrine pancreas causing secretion of these hormones. In hypophysectomized dogs, basal levels of plasma insulin and glucagon were decreased and neurotensin had little effect on the endocrine pancreas even with the administration of a large dose.</p>
Keywords neurotensin insulin glucagon hypophysectomy
Amo Type Article
Published Date 1978-08
Publication Title Acta Medica Okayama
Volume volume32
Issue issue4
Publisher Okayama University Medical School
Start Page 301
End Page 308
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 153091
NAID 120002312574
JaLCDOI 10.18926/AMO/32692
FullText URL fulltext.pdf
Author Okada, Soji| Kawanishi, Koichi| Ofuji, Tadashi|
Amo Type Brief Note
Published Date 1974-08
Publication Title Acta Medica Okayama
Volume volume28
Issue issue4
Publisher Okayama University Medical School
Start Page 295
End Page 297
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4280239
NAID 120002312128
JaLCDOI 10.18926/AMO/32039
FullText URL fulltext.pdf
Author Yamana, Seizo| Nakamura, Zenichi| Saito, Yoshihito| Yamamoto, Michinori| Ofuji, Tadashi|
Abstract <p>Purification of antilymphocyte antibody (ALA) from patients with systemic lupus erythematosus (SLE) was achieved by immunoabsorption and elution. Human tonsil cells or thymocytes were used as absorbents. Complement dependent microcytotoxicity tests showed that, in comparison to the parent sera, the eluate from tonsil cells was eight times, and that from thymocytes four times, more active. Antinuclear activity was eliminated by elution. The ALA was almost entirely IgM, IgG being involved in only a few cases. IgA lacked cytotoxic activity. ALA was directed at both T- and B-cell surface determinants, which suggests that, in SLE, it has a heterogeneous biological composition.</p>
Keywords antilymphocyte antibody systemic lupus erythematosus purification
Amo Type Article
Published Date 1979-08
Publication Title Acta Medica Okayama
Volume volume33
Issue issue4
Publisher Okayama University Medical School
Start Page 259
End Page 267
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 91309
JaLCDOI 10.18926/AMO/32075
FullText URL fulltext.pdf
Author Mitsunaga, Mikio| Hasegawa, Kan| Nakagawa, Osamu| Nakashima, Yusaku| Miyoshi, Masanori| Suzuki, Shinya| Ofuji, Tadashi|
Abstract <p>A case of a 40-year-old woman who was suffering from painless thyroiditis with recurrent transient thyrotoxicosis is reported. Acute exacerbations occurred four times during the past ten years, two after delivery and two after catching a cold. Serum thyroid hormones increased, though radioiodine uptake by the thyroid was very low and no inflammatory signs were observed. The histological findings of the thyroid were of atypical thyroiditis and not consistent with either chronic lymphocytic thyroiditis or subacute thyroiditis. Tanned sheep red cell hemagglutination titers for anti-thyroglobulin antibodies (TRC) and for anti-microsomal antibodies (MHA) were negative or low. The disease seems to be rare and the pathophysiology and etiology are discussed.</p>
Keywords recurrent transient thyrotoxicosis painless thyroiditis hyper-thyroiditis subacute thyroiditis chronic lymphocytic thyroiditis
Amo Type Article
Published Date 1979-02
Publication Title Acta Medica Okayama
Volume volume33
Issue issue1
Publisher Okayama University Medical School
Start Page 43
End Page 51
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 155981
JaLCDOI 10.18926/AMO/31419
FullText URL fulltext.pdf
Author Nishiya, Koji| Yamana, Seizo| Sarai, Tetsuo| Ota, Zensuke| Ofuji, Tadashi|
Abstract <p>The surface structure of myeloma cells was examined by scanning electron microscopy. The cells were collected from the pleural effusion of a multiple myeloma patient and purified by Conray-Ficoll gradient sedimentation. The cell size ranged from 8 mu to 12 mu in diameter and the microvilli were from 0.8 mu to 1.2 mu in length. The surfaces of the majority of the observed myeloma cells were more villous than lymphocytes.</p>
Amo Type Brief Note
Published Date 1976-04
Publication Title Acta Medica Okayama
Volume volume30
Issue issue2
Publisher Okayama University Medical School
Start Page 143
End Page 145
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 135486
NAID 120002313112
JaLCDOI 10.18926/AMO/32053
FullText URL fulltext.pdf
Author Hashimoto, Kozo| Yunoki, Sho| Hosogi, Hidemi| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>Biologically active peptides and neurotransmitter substances were added to anterior pituitary cell cultures to examine the presence of corticotropin releasing factor (CRF)-like activity. Hypothalamic extract (HE) induced significant dose-related increase of ACTH, and the lowest effective dose was 0.01 HE/ml. Other tested substances including luteinizing hormone-releasing hormone, thyrotropin releasing hormone, melanocyte stimulating hormone release inhibiting factor, somatostatin, substance P, neurotensin, beta-endorphin. leu-enkephalin, met-enkephalin, bradykinin, norepinephrine, dopamine, serotonin, acetylcholine, histamine, gamma-amino butyric acid or gamma-hydroxy butyric acid showed no CRF-like activity. Relatively high doses of lysine vasopressin, arginine vasopressin and angiotensin II increased the release of ACTH in pituitary cell cultures, but the maximal ACTH response was markedly less than with HE. These results indicate that cultured anterior pituitary cells are sensitive and fairly specific in detecting CRF(s) comparing with other detecting procedures.</p>
Keywords pituitary cell cultures corticotropin releasing factor ACTH neuropeptides neurotransmitter substances
Amo Type Article
Published Date 1979-04
Publication Title Acta Medica Okayama
Volume volume33
Issue issue2
Publisher Okayama University Medical School
Start Page 81
End Page 90
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 38634
NAID 120002311394
JaLCDOI 10.18926/AMO/32708
FullText URL fulltext.pdf
Author Nishiya, Koji| Yamana, Seizo| Yano, Keisuke| Ensako, Katuhide| Fujiwara, Tadaaki| Ofuji, Tadashi|
Amo Type Brief Note
Published Date 1974-10
Publication Title Acta Medica Okayama
Volume volume28
Issue issue5
Publisher Okayama University Medical School
Start Page 373
End Page 375
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4281998
JaLCDOI 10.18926/AMO/30561
FullText URL fulltext.pdf
Author Miyoshi, Masanori| Nakashima, Yusaku| Hirakawa, Shuzo| Mitsunaga, Mikio| Nakagawa, Osamu| Miura, Hiroshi| Suzuki, Shinya| Ofuji, Tadashi|
Abstract <p>Function of pituitary-thyroid axis was studied in rats with experimentally induced thyroiditisWistar strain female rats were immunized with homologous thyroid extract in Freund's complete adjuvant and with simultaneous intradermal injection of pertussis vaccine concentrate. They received booster shots at the first and third week after the initial immunization. Serum thyroid hormones and TSH were measured just before, and at weekly intervals after, the initial immunization. Histological examination of the thyroid gland at the second week after immunization showed slight infiltration of macrophages in the thyroid follicles. From the third to the fourth week, massive lymphoid cell infiltration and destruction of follicular architecture developed in all immunized rats. Serum R3 levels slightly decreased during the second week, increased transiently during the third week, then decreased again thereafter. Serum T4 levels decreased slightly durinf the fourth week. Serum TSH levels were not elevated significantly during the third week, but the response to TRH was significantly increased at this time. Basal TSH levels were increased during the fourth week. The TRH test was a sensitive method capable of detecting minimal failure of thyroid function undetected by other routine measuremens of thyroid hormones and TSH.</p>
Keywords thyroid function thyrotropin (TSH) experimental thyroiditis thyroid hormones.
Amo Type Article
Published Date 1980-02
Publication Title Acta Medica Okayama
Volume volume34
Issue issue1
Publisher Okayama University Medical School
Start Page 11
End Page 17
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6446835
Web of Science KeyUT A1980JS13800002
Author Ofuji, Tadashi| Yamachika, Yukio| Fukuda, Genjiro| Kamura, Junta| Kotani, Saburo| Ota, Zensuke| Kitajima, Koichi|
Published Date 1959-06
Publication Title Acta Medicinae Okayama
Volume volume13
Issue issue2
Content Type Journal Article
Author Hiraki, Kiyoshi| Ofuji, Tadashi| Sunami, Hiroshi|
Published Date 1956-07
Publication Title Acta Medicinae Okayama
Volume volume10
Issue issue3
Content Type Journal Article
Author Hiraki, Kiyoshi| Ofuji, Tadashi| Sunami, Hiroshi| Ota, Zensuke| Kitajima, Koichi| Shinagawa, Koji| Kibata, Masayoshi| Miyoshi, Isao| Hanazawa, Atsumasa|
Published Date 1963-02
Publication Title Acta Medicinae Okayama
Volume volume17
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/32052
FullText URL fulltext.pdf
Author Makino, Hirofumi| Ota, Zensuke| Takaya, Yasumasa| Kida, Keiko| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
Abstract <p>The tubular basement membrane (TBM) (i.e. tubular basal lamina) of rat kidney was shown to be a fine meshwork by electron microscopy after negative staining. Strands of the meshwork formed a regular three dimensional lattice work. The pores of the meshwork were polygonal. There were two main pore sizes: one approximately 30 A in diameter, the other 42--60 A. In view of our previous observation that glomerular and alveolar basement membranes were made up fine meshwork, it is quite possible that the basement membranes of other organs are also made up such fine meshwork.</p>
Keywords renal tubular basement membrane mesh work structure pore negative staining electron micro scopy
Amo Type Brief Note
Published Date 1979-04
Publication Title Acta Medica Okayama
Volume volume33
Issue issue2
Publisher Okayama University Medical School
Start Page 133
End Page 136
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 89787
NAID 120002312032
JaLCDOI 10.18926/AMO/30838
FullText URL fulltext.pdf
Author Okada, Soji| Yagyu, Fumito| Kawanishi, Koichi| Ofuji, Tadashi|
Abstract <p>The relationship between immune insulitis and glucose tolerance was investigated in three groups of mice following active immunization with different components of bovine pancreatic hormone. An abnormal blood glucose level was observed in the three groups ranging from 33.3% to 87.5% of sensitized mice. A relationship was not present between the glucose tolerance response and the presence of insulitis or anti-insulin antibody in the blood of sensitized mice. However, all sensitized mice with a marked decrease in glucose tolerance were found to have insulitis. In animals without established insulitis and with no demonstrable anti-insulin antibody, abnormal glucose tolerance was noted. This latter condition occurred more frequently with recrystallized insulin than with a-component and did not occur with monocomponent insulin. These findings seemed to indicate that two distinct processes involving some circulating antibodies with anti-insulin antibody and insulitis might be involved in the development of the observed glucose tolerance abnormality.</p>
Amo Type Article
Published Date 1977-06
Publication Title Acta Medica Okayama
Volume volume31
Issue issue3
Publisher Okayama University Medical School
Start Page 211
End Page 216
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 144420
NAID 120002305762