Author Okada, Soji| Kawanishi, Koichi| Ofuji, Tadashi|
Published Date 1974-10
Publication Title Acta Medica Okayama
Volume volume28
Issue issue5
Content Type Journal Article
Author Nishiya, Koji| Yamana, Seizo| Yano, Keisuke| Ensako, Katuhide| Fujiwara, Tadaaki| Ofuji, Tadashi|
Published Date 1974-10
Publication Title Acta Medica Okayama
Volume volume28
Issue issue5
Content Type Journal Article
Author Kawanishi, Koichi| Sato, Keiichiro| Ofuji, Tadashi| Ogawa, Katsuo|
Published Date 1974-12
Publication Title Acta Medica Okayama
Volume volume28
Issue issue6
Content Type Journal Article
Author Okada, Soji| Kawanishi, Koichi| Ofuji, Tadashi|
Published Date 1974-08
Publication Title Acta Medica Okayama
Volume volume28
Issue issue4
Content Type Journal Article
Author Hiraki, Kiyoshi| Ofuji, Tadashi| Sunami, Hiroshi| Ota, Zensuke| Kitajima, Koichi| Shinagawa, Koji| Kibata, Masayoshi| Miyoshi, Isao| Hanazawa, Atsumasa|
Published Date 1963-02
Publication Title Acta Medicinae Okayama
Volume volume17
Issue issue1
Content Type Journal Article
Author Ofuji, Tadashi|
Published Date 1964-04
Publication Title Acta Medicinae Okayama
Volume volume18
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/32075
FullText URL fulltext.pdf
Author Mitsunaga, Mikio| Hasegawa, Kan| Nakagawa, Osamu| Nakashima, Yusaku| Miyoshi, Masanori| Suzuki, Shinya| Ofuji, Tadashi|
Abstract <p>A case of a 40-year-old woman who was suffering from painless thyroiditis with recurrent transient thyrotoxicosis is reported. Acute exacerbations occurred four times during the past ten years, two after delivery and two after catching a cold. Serum thyroid hormones increased, though radioiodine uptake by the thyroid was very low and no inflammatory signs were observed. The histological findings of the thyroid were of atypical thyroiditis and not consistent with either chronic lymphocytic thyroiditis or subacute thyroiditis. Tanned sheep red cell hemagglutination titers for anti-thyroglobulin antibodies (TRC) and for anti-microsomal antibodies (MHA) were negative or low. The disease seems to be rare and the pathophysiology and etiology are discussed.</p>
Keywords recurrent transient thyrotoxicosis painless thyroiditis hyper-thyroiditis subacute thyroiditis chronic lymphocytic thyroiditis
Amo Type Article
Published Date 1979-02
Publication Title Acta Medica Okayama
Volume volume33
Issue issue1
Publisher Okayama University Medical School
Start Page 43
End Page 51
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 155981
JaLCDOI 10.18926/AMO/32053
FullText URL fulltext.pdf
Author Hashimoto, Kozo| Yunoki, Sho| Hosogi, Hidemi| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>Biologically active peptides and neurotransmitter substances were added to anterior pituitary cell cultures to examine the presence of corticotropin releasing factor (CRF)-like activity. Hypothalamic extract (HE) induced significant dose-related increase of ACTH, and the lowest effective dose was 0.01 HE/ml. Other tested substances including luteinizing hormone-releasing hormone, thyrotropin releasing hormone, melanocyte stimulating hormone release inhibiting factor, somatostatin, substance P, neurotensin, beta-endorphin. leu-enkephalin, met-enkephalin, bradykinin, norepinephrine, dopamine, serotonin, acetylcholine, histamine, gamma-amino butyric acid or gamma-hydroxy butyric acid showed no CRF-like activity. Relatively high doses of lysine vasopressin, arginine vasopressin and angiotensin II increased the release of ACTH in pituitary cell cultures, but the maximal ACTH response was markedly less than with HE. These results indicate that cultured anterior pituitary cells are sensitive and fairly specific in detecting CRF(s) comparing with other detecting procedures.</p>
Keywords pituitary cell cultures corticotropin releasing factor ACTH neuropeptides neurotransmitter substances
Amo Type Article
Published Date 1979-04
Publication Title Acta Medica Okayama
Volume volume33
Issue issue2
Publisher Okayama University Medical School
Start Page 81
End Page 90
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 38634
NAID 120002311394
JaLCDOI 10.18926/AMO/32052
FullText URL fulltext.pdf
Author Makino, Hirofumi| Ota, Zensuke| Takaya, Yasumasa| Kida, Keiko| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
Abstract <p>The tubular basement membrane (TBM) (i.e. tubular basal lamina) of rat kidney was shown to be a fine meshwork by electron microscopy after negative staining. Strands of the meshwork formed a regular three dimensional lattice work. The pores of the meshwork were polygonal. There were two main pore sizes: one approximately 30 A in diameter, the other 42--60 A. In view of our previous observation that glomerular and alveolar basement membranes were made up fine meshwork, it is quite possible that the basement membranes of other organs are also made up such fine meshwork.</p>
Keywords renal tubular basement membrane mesh work structure pore negative staining electron micro scopy
Amo Type Brief Note
Published Date 1979-04
Publication Title Acta Medica Okayama
Volume volume33
Issue issue2
Publisher Okayama University Medical School
Start Page 133
End Page 136
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 89787
NAID 120002312032
JaLCDOI 10.18926/AMO/32041
FullText URL fulltext.pdf
Author Yokoyama, Hisamitsu| Yumane, Yukio| Takahara, Jiro| Yoshinouchi, Takeo| Ofuji, Tadashi|
Abstract <p>An unusual case of orbital tumor with high renin content and severe hypertension is described. The patient was a 15-year-old girl with juvenile hypertension (200-140 mmHg) associated with right exophthalmos and hypokalemia. The patient showed extremely high levels of plasma renin activity and plasma aldosterone concentration. No difference was present in plasma renin activity from either side of the renal veins. Preoperatively, hypertension responded to treatment with spironolactone. The tumor could not be completely removed because of intracranial metastasis and infiltration, and the hyperreninemia and secondary hyperaldosteronism persisted. The renin content in the orbital tissue was 1,403-2,225 ng/angiotensin I generated/h/g wet weight of tissue. The postmortem histopathologic diagnosis was orbital hemangiopericytoma. This is the first case of extrarenal (ectopic) renin-secreting (or -producing) hemangiopericytoma of the orbital origin. Furthermore this case is worthy of note in the point of view of the presence of the extrarenal renin-angiotensin system, particularly in the brain.</p>
Keywords renin producing tumor hemangiopericytoma juvenile hypertension hypokalemia orbital tumor
Amo Type Brief Note
Published Date 1979-08
Publication Title Acta Medica Okayama
Volume volume33
Issue issue4
Publisher Okayama University Medical School
Start Page 315
End Page 322
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 158952
NAID 120002311859
JaLCDOI 10.18926/AMO/32039
FullText URL fulltext.pdf
Author Yamana, Seizo| Nakamura, Zenichi| Saito, Yoshihito| Yamamoto, Michinori| Ofuji, Tadashi|
Abstract <p>Purification of antilymphocyte antibody (ALA) from patients with systemic lupus erythematosus (SLE) was achieved by immunoabsorption and elution. Human tonsil cells or thymocytes were used as absorbents. Complement dependent microcytotoxicity tests showed that, in comparison to the parent sera, the eluate from tonsil cells was eight times, and that from thymocytes four times, more active. Antinuclear activity was eliminated by elution. The ALA was almost entirely IgM, IgG being involved in only a few cases. IgA lacked cytotoxic activity. ALA was directed at both T- and B-cell surface determinants, which suggests that, in SLE, it has a heterogeneous biological composition.</p>
Keywords antilymphocyte antibody systemic lupus erythematosus purification
Amo Type Article
Published Date 1979-08
Publication Title Acta Medica Okayama
Volume volume33
Issue issue4
Publisher Okayama University Medical School
Start Page 259
End Page 267
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 91309
JaLCDOI 10.18926/AMO/32035
FullText URL fulltext.pdf
Author Miyoshi, Akira| Makino, Hirofumi| Hiramatsu, Makoto| Takahashi, Kayo| Yamane, Yukio| Takahara, Jiro| Suzuki, Shinya| Ota, Zensuke| Ofuji, Tadashi| Takehisa, Yoshiaki|
Abstract <p>A 26-year-old female with Bartter's syndrome associated with Graves' disease is reported. This patient had a history of Graves' disease from the age of 22 and anti-thyroid drug (Methimazole) had been administered for 2 years. Thyroid function returned to normal but general fatigue and polyuria continued. Hypokalemia was diagnosed at 25 years of age and she was referred to our hospital for evaluation. Blood pressure was normal and laboratory data revealed normal thyroid function, hypokalemic alkalosis, high plasma renin activity and high plasma aldosterone concentration. She showed normal pressor sensitivity to norepinephrine infusion, grossly diminished pressor sensitivity to exogenous angiotensin II infusion compared with the normal. A renal biopsy specimen showed juxtaglomerular cell hyperplasia. Electron microscopy confirmed lacis cell (agranular cell) proliferation.</p>
Keywords Bartter's syndrome juxtaglomerular cell hyperplasia secondary aldosteronism plasma renin activity
Amo Type Article
Published Date 1979-08
Publication Title Acta Medica Okayama
Volume volume33
Issue issue4
Publisher Okayama University Medical School
Start Page 305
End Page 314
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 158951
Author Yamamoto, Michio| Tanimoto, Junichi| Kojima, Sumikazu| Nobuki, Shigeo| Hagitani, Akira| Iguchi, Yoshiko| Akagi, Eiko| Ofuji, Tadashi| Asaka, Takakazu| Kamura, Junta| Shibata, Tsuneo|
Published Date 1960-04
Publication Title Acta Medicinae Okayama
Volume volume14
Issue issue1
Content Type Journal Article
Author Nishiya, Koji| Yamana, Seizo| Sarai, Tetsuo| Ota, Zensuke| Ofuji, Tadashi|
Published Date 1976-04
Publication Title Acta Medica Okayama
Volume volume30
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/31293
FullText URL fulltext.pdf
Author Hashimoto, Kozo| Ohno, Norihito| Aoki, Yoshiyuki| Kageyama, Jingo| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>The distribution of corticotropin releasing factor (CRF) and arginine vasopressin (AVP) in hypothalamic nuclei were examined in control and estrogen-treated female rats. CRF activity was measured using monolayer cultured rat anterior pituitary cells and AVP by radioimmunoassay. Hypothalamic nuclei were punched out according to the method of Palkovits. The distribution of CRF activity in 5 different hypothalamic nuclei was similar to that of AVP in intact female rats. CRF activity in hypothalamic nuclei, pituitary ACTH content and plasma ACTH levels in estrogen-treated rats were not significantly different from those in control rats. However, significant elevation of AVP content was observed in the supraoptic and paraventricular nuclei of estrogen-treated rats. These results indicate that CRF and AVP are distributed in similar hypothalamic nuclei, but that they are not identical.</p>
Keywords corticotropin releasing factor (CRF) arginine vasopressin (AVP) hypothalamic nucler.
Amo Type Article
Published Date 1981-02
Publication Title Acta Medica Okayama
Volume volume35
Issue issue1
Publisher Okayama University Medical School
Start Page 37
End Page 43
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6266205
Web of Sience KeyUT A1981LH76300004
JaLCDOI 10.18926/AMO/31292
FullText URL fulltext.pdf
Author Ohno, Norihito| Hashimoto, Kozo| Yunoki, Sho| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>A perifusion method has been developed using rat hypothalamo-neurohypophyseal system (HNS) or neural lobe to investigate the control mechanism of arginine vasopressin (AVP) release. A specific radioimmunoassay (RIA) for AVP was developed to measure AVP in perifusion medium employing anti-AVP serum which was obtained by immunizing rabbits. At a final dilution of 1/12,000, the antiserum showed less than 0.66 and 0.01% cross reactivity with lysine-vasopressin and oxytocin, respectively. But it did not cross reacted with other peptide hormones. The lowest detectable level of vasopressin was 0.5 pg/tube. The intra-assay coefficient of variation averaged 10.4%. The dilution curve of perifused medium was well paralled to the standard curve of AVP assay. AVP release from HNS or neural lobe gradually declined to the stable level in 90-120 min after the initiation of perifusion. Good repeatability of the AVP release from neural lobe was recognized by repeated stimulation with 10 min perifusion of 60 mM KCl at every 60 min. HNS released AVP in dose related manner to the osmotic challenge of sodium or glucose, and AVP release was stimulated from HNS by prostaglandin E2, but not by dopamine. These results show that the perifusion methods using AVP-RIA is a useful method to examine the AVP release from HNS or neural lobe.</p>
Amo Type Article
Published Date 1981-02
Publication Title Acta Medica Okayama
Volume volume35
Issue issue1
Publisher Okayama University Medical School
Start Page 27
End Page 35
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6455045
Web of Science KeyUT A1981LH76300003
JaLCDOI 10.18926/AMO/31291
FullText URL fulltext.pdf
Author Ohno, Norihito| Hashimoto, Kozo| Yunoki, Sho| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>Chromosome studies were conducted on two patients with adult T-cell leukemia. In both patients, a marker chromosome 14q+ and a structural change involving chromosome 1 with trisomy of the q arm were found in peripheral blood leukocytes. The 14q+ marker chromosome had resulted from translocation from #5p in one patient and #5q in the other patient. The present and previous studies suggest that the donor chromosomes involved in the 14q+ translocation are variable. This indicates that the 14q+ marker chromosome rather than the donor chromosome is intimately related with adult T-cell leukemia.</p>
Amo Type Article
Published Date 1981-02
Publication Title Acta Medica Okayama
Volume volume35
Issue issue1
Publisher Okayama University Medical School
Start Page 19
End Page 25
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6455044
Web of Science KeyUT A1981LH76300002
JaLCDOI 10.18926/AMO/31290
FullText URL fulltext.pdf
Author Hashimoto, Kozo| Ohno, Norihito| Aoki, Yoshiyuki| Kageyama, Jingo| Takahara, Jiro| Ofuji, Tadashi|
Abstract <p>The distribution of corticotropin releasing factor (CRF) and arginine vasopressin (AVP) in hypothalamic nuclei were examined in control and estrogen-treated female rats. CRF activity was measured using monolayer cultured rat anterior pituitary cells and AVP by radioimmunoassay. Hypothalamic nuclei were punched out according to the method of Palkovits. The distribution of CRF activity in 5 different hypothalamic nuclei was similar to that of AVP in intact female rats. CRF activity in hypothalamic nuclei, pituitary ACTH content and plasma ACTH levels in estrogen-treated rats were not significantly different from those in control rats. However, significant elevation of AVP content was observed in the supraoptic and paraventricular nuclei of estrogen-treated rats. These results indicate that CRF and AVP are distributed in similar hypothalamic nuclei, but that they are not identical.</p>
Keywords corticotropin releasing factor (CRF) arginine vasopressin (AVP) hypothalamic nucler.
Amo Type Article
Published Date 1981-02
Publication Title Acta Medica Okayama
Volume volume35
Issue issue1
Publisher Okayama University Medical School
Start Page 37
End Page 43
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6266205
Web of Science KeyUT A1981LH76300004
JaLCDOI 10.18926/AMO/31275
FullText URL fulltext.pdf
Author Asano, Taro| Yano, Keisuke| Ofuji, Tadashi|
Abstract <p>To investigate cellular interactions between human T and B lymphocytes in various diseases, we established a technique to prove terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. We also established a double antibody radioimmunoassay to measure the amount of IgG, IgA and IgM synthesized and secreted in culture supernatants. Purified immunoglobulins were obtained from sera of patients with myeloma or macroglobulinemia. The peripheral blood lymphocytes from 25 normal individuals had the geometric mean synthetic rates of 1886 ng for IgG, 1607 ng for IgA and 1173 ng for IgM per 1 X 10(6) cells when cultured for nine days in the presence of pokeweed mitogen. The method is simple and sensitive, and is thought to be useful for examining human lymphocyte function in vitro.</p>
Keywords lymphocytes PWM immunoglobulins RIA.
Amo Type Article
Published Date 1981-11
Publication Title Acta Medica Okayama
Volume volume35
Issue issue5
Publisher Okayama University Medical School
Start Page 319
End Page 326
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6458995
Web of Science KeyUT A1981MS42400002
JaLCDOI 10.18926/AMO/31274
FullText URL fulltext.pdf
Author Makino, Hirofumi| Takaoka, Michio| Komoda, Keizo| Tokuyama, Kiyoyuki| Ota, Zensuke| Ofuji, Tadashi| Kaneshige, Tetsuji|
Abstract <p>Masugi nephritis was induced in rats by a single intravenous injection of rabbit anti-rat kidney serum, and studied with a scanning electron microscope. A characteristic finding was the presence of white cells, probably polymorphonuclear leukocytes, with many microspikes which penetrated through degenerated glomerular endothelial cells to be in direct contact with the glomerular basement membrane. This finding confirms the pathogenic role of leukocytes in glomerulonephritis induced by anti-glomerular basement membrane antibody.</p>
Keywords Masugi nephritis scanning electron microscopy leukocyte.
Amo Type Brief Note
Published Date 1981-11
Publication Title Acta Medica Okayama
Volume volume35
Issue issue5
Publisher Okayama University Medical School
Start Page 373
End Page 376
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6459000
Web of Science KeyUT A1981MS42400007