このエントリーをはてなブックマークに追加
ID 33024
FullText URL
Author
Burakham, Rodjana
Oshima, Mitsuko
Grudpan, Kate
Motomizu, Shoji
Abstract

A novel spectrophotometric reaction system was developed for the determination of nitrite as well as nitrate in water samples, and was applied to a flow-injection analysis (FIA). The spectrophotometric flow-injection system coupled with a copperised cadmium reductor column was proposed. The detection was based on the nitrosation reaction between nitrite ion and phloroglucinol (1,3,5-trihydroxybenzene), a commercially available phenolic compound. Sample injected into a carrier stream was split into two streams at the Y-shaped connector. One of the streams merged directly and reacted with the reagent stream: nitrite ion in the samples was detected. The other stream was passed through the copperised cadmium reductor column, where the reduction of nitrate to nitrite occurred, and the sample zone was then mixed with the reagent stream and passed through the detector: the sum of nitrate and nitrite was detected. The optimised conditions allow a linear calibration range of 0.03-0.30 mug NO2--N ml(-1) and 0.10-1.00 mug NO3--N ml(-1). The detection limits for nitrite and nitrate, defined as three times the standard deviation of measured blanks are 2.9 ng NO2--N ml(-1) and 2.3 ng NO3--N ml(-1), respectively. Up to 20 samples can be analyzed per hour with a relative standard deviation of less than 1.5%. The proposed method could be applied successfully to the simultaneous determination of nitrite and nitrate in water samples.

Keywords
Flow-injection analysis
Phloroglucinol
Nitrite
Nitrate
Simultaneous determination
Note
Published with permission from the copyright holder.
This is a author's copy,as published in TALANTA , 2004 Vol.64 Issue.5 pp.1259-1265
Publisher URL: http://dx.doi.org/10.1016/j.talanta.2004.03.059
Direct access to Thomson Web of Science record
(C) 2004 by Elsevier B.V
Published Date
2004-12-15
Publication Title
Talanta
Volume
volume64
Issue
issue5
Start Page
1259
End Page
1265
Content Type
Journal Article
language
英語
Refereed
True
DOI
Web of Sience KeyUT
Submission Path
analytical_chemistry/21