JaLCDOI 10.18926/AMO/32689
FullText URL fulltext.pdf
Author Orita, Kunzo| Miwa, Hiroaki| Kaneda, Shoken|
Abstract <p>By dividing at random 14 normal persons into 7 pairs of two individuals each, lymphocytes were isolated from their peripheral blood and taking one of the pairs as stimulating cells or antigens and the others as responding cells, mixed lymphocyte culture (MLC) was carried out. As for the method of MLC we used our MLC method of unidirectional mixed culture with a small amount of lymphocytes in additition of 1% (v /v) PHA.M and cultured for three days, and a widely used conventional method in which 3H.thymidine uptake was the parameter of the blastformation rate and cultured for seven days. In comparing the results of these two groups of MLC the data in six experiments out of the seven coincided. Namely, with 5x 104 cells each of stimulating cell group and responding cell group, it is possible to achieve satisfactory MLC, the culture can be done only for three days without requiring any special technique and the results can be readily evaluated. Therefore, MLC by our simple method would yield satisfactory results in clinics.</p>
Amo Type Article
Published Date 1974-08
Publication Title Acta Medica Okayama
Volume volume28
Issue issue4
Publisher Okayama University Medical School
Start Page 253
End Page 257
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4280235
NAID 120002311525
JaLCDOI 10.18926/AMO/32683
FullText URL fulltext.pdf
Author Sakagami, Kenichi| Saito, Shinya| Shiozaki, Shigehiro| Takasu, Shinji| Matsuno, Tsuyoshi| Fujiwara, Takuzo| Kusaka, Satoshi| Uda, Masashi| Matsuoka, Junji| Naomoto, Yoshio| Gouchi, Akira| Hamazaki, Keisuke| Tanaka, Shinichiro| Orita, Kunzo|
Abstract <p>A retrospective study was carried out in 110 cadaveric kidney transplant recipients to compare the effects of low doses of cyclosporine (CsA), azathioprine (AZP) and steroids (triple-drug therapy) with those of higher doses of steroids plus AZP (conventional immunosuppression). Graft survival rate in the triple-drug therapy was 77%, 69%, and 69% at 1, 3, and 5 years, respectively. This was significantly better than 48%, 34%, and 29% in conventional immunosuppression. The incidence of acute rejection episodes was significantly lower in the triple-drug therapy than in conventional immunosuppression (25% vs 58%). In conclusion, our study shows that triple-drug therapy using low-dose cyclosporine is the safest of the immunosuppressive regimens and provides a beneficial effect on the long-term survival of cadaveric kidney transplants.</p>
Keywords cadaveric kindney transplantation cyclosporine triple-drug therapy
Amo Type Article
Published Date 1992-02
Publication Title Acta Medica Okayama
Volume volume46
Issue issue1
Publisher Okayama University Medical School
Start Page 53
End Page 56
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1561907
Web of Science KeyUT A1992HH01700010
JaLCDOI 10.18926/AMO/32682
FullText URL fulltext.pdf
Author Moreira, Luis Fernando| Iwagaki, Hiromi| Inoguchi, Kazuhito| Hizuta, Akio| Sakagami, Keinichi| Orita, Kunzo|
Abstract <p>Thirteen patients with rectal carcinoma seen between December 1980 and December 1990 have been reviewed to determine the risk of lymph node metastasis and its implication for subsequent treatment. The mean age was 64 years (from 38 to 79; 9 males, 4 females). The site of the tumor was predominantly in the lower rectum (53.8 percent). The polypoid (I) and flat-elevated ulcerated (IIa + IIc) subtypes were detected in seven and six lesions, respectively. Sphincter-saving techniques were carried out in eight cases, and five cases required Miles' operation. Neither postoperative complications nor deaths were noted. The mean follow-up period was 57 months (6 to 133 months). No recurrence or distant metastasis was found during this follow-up. IIa + IIc subtype lesions with deep submucosal invasion at or beyond Smlc level were closely related with lymphatic and vascular invasion. Although this association was not necessarily accompanied by an increased number of involved lymph nodes, major surgical resection is suggested in such IIa + IIc cases due to an increased possibility for lymph node metastasis.</p>
Keywords early rectal carcinoma invasive carcinoma submucosal invasion surgical treatment metastasis survival
Amo Type Article
Published Date 1992-02
Publication Title Acta Medica Okayama
Volume volume46
Issue issue1
Publisher Okayama University Medical School
Start Page 7
End Page 10
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1561908
Web of Science KeyUT A1992HH01700002
JaLCDOI 10.18926/AMO/32678
FullText URL fulltext.pdf
Author Sakagami, Kenichi| Saito, Shinya| Shiozaki, Shigehiro| Fujiwara, Takuzo| Haisa, Minoru| Niguma, Takefumi| Kusaka, Satoshi| Uda, Masashi| Matsuno, Tsuyoshi| Takasu, Shinji| Yerdel, Mehmet Ali| Matsuoka, Junji| Tanaka, Shinichiro| Orita, Kunzo|
Abstract <p>One-hundred-nine HLA-haploidentical living related renal transplants have been retrospectively analysed to compare the effect of donor-specific blood transfusion (DST) and different immunosuppressive regimens on graft survival and acute rejection. The recipients were divided into four groups according to the immunosuppressive therapy. Group 1 (n = 44): conventional therapy with posttransplant azathioprine (AZP) + methylprednisolone (MP). Group 2 (n = 25): pretransplant DST + posttransplant AZP + MP. Group 3 (n = 12): triple-drug therapy with posttransplant AZP + MP + cyclosporine (CS). Group 4 (n = 25): pretransplant DST + posttransplant AZP + MP + CS. The five-year actuarial survival rates for groups 1, 2, 3 and 4 were 48%, 73%, 79%, and 89%, respectively. The graft survival rate in group 3 was significantly (p less than 0.01) better than that in group 1. The transfusion effect was reduced, and appears as a 10% improvement in the graft survival in the cyclosporin era compared with a 25% improvement at pre-cyclosporin era. Furthermore, the incidence of the first rejection episode was decreased in recipients that received DST. The present study revealed that DST, as pretransplant conditioning has a definite impact on rejection-free long-term graft survival in HLA-haploidentical living-related kidney recipients and the most favorable outcome in such patients could be achieved by DST pretreatment in conjunction with posttransplant triple-drug therapy including cyclosporine.</p>
Keywords living-related kindney transplantation donor-specific blood transfusion (DST) cyclosporine
Amo Type Article
Published Date 1992-02
Publication Title Acta Medica Okayama
Volume volume46
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 5
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1561899
Web of Science KeyUT A1992HH01700001
JaLCDOI 10.18926/AMO/32645
FullText URL fulltext.pdf
Author Orita, Kunzo| Fuchimoto, Sadanori| Kurimoto, Masashi| Ando, Shunsaku| Minowada, Jun|
Abstract <p>Synergistic enhancement of anti-tumor effects through the combined use of natural human interferon-alpha (nHuIFN-alpha) and natural human tumor necrosis factor-alpha (nHuTNF-alpha) enabled us to decrease the effective dose of each cytokine and consequently to reduce side effects. One hundred and twenty patients with advanced or recurrent solid cancer were entered in the trial from April 1985 to January 1988, of whom 112 patients were evaluable. A mixture of nHuINF-alpha and nHuTNF-alpha was injected intravenously as the maintenance dose 1 x 10(6)U or more/day for over 8 weeks. There was no response in 40 patients injected with the maintenance dose of 1 x 10(6)U/day, but of 72 patients receiving more than 2 x 10(6)U/day (10 micrograms of nHuIFN-alpha and 3 micrograms of nHuTNF-alpha), 4 had complete responses, 10 had partial responses, and 4 had minor responses. The overall response rate was 12.5% (14/112) and the rate was 19.5% in 72 patients with more than 2 x 10(6)U/day. Positive responses were as follows: hepatoma 3/8), renal cell cancer (4/11), breast cancer (4/17), ovarian cancer (1/2), malignant thymoma (1/1) and liposarcoma (1/1). Serious adverse effects like hypotension, oliguria and severe hepatobiliary toxicity were never experienced. The effective and adequate dose of the mixed preparation was considered 2 to 4 x 10(6)U/day/body.</p>
Keywords interferon-? tumor necrosis factor-? cancer combination therapy early phase ?
Amo Type Article
Published Date 1992-04
Publication Title Acta Medica Okayama
Volume volume46
Issue issue2
Publisher Okayama University Medical School
Start Page 103
End Page 112
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1575056
Web of Science KeyUT A1992HR48400007
JaLCDOI 10.18926/AMO/32642
FullText URL fulltext.pdf
Author Bai, Liyan| Naomoto, Yoshio| Miyazaki, Masahiro| Orita, Kunzo| Namba, Masayoshi|
Abstract <p>The present experiment was undertaken to study what types of human cancers are responsive to the antiproliferative effects of suramin. The human malignant cells used were as follows: cervical cancer (HeLa), mammary cancer (MCF-7), bladder cancer (EJ), hepatoma (HuH-7, PLC/PRF/5), embryonal carcinoma (PA-1), in vitro transformed fibroblasts (KMST-6, SUSM-1, VA-13), five myeloma cell lines (KMM-1, KMS-5, KMS-11, KMS-12, RPMI 8226), Burkitt's lymphoma (Raji), acute promyelocytic leukemia (HL-60), chronic myelocytic leukemia (K562), Epstein-Barr virus nuclear antigen positive lymphoblastoid cells (KMS-9). The cells were treated with 25 to 100 micrograms/ml suramin for 72h. Proliferation of HuH-7 and two human myeloma cells (KMS-11 and KMS-12) was remarkably inhibited, and that of PA-1, PLC/PRF/5, KMST-6, two other myeloma cell lines (KMM-1 and KMS-5), Raji and HL-60, was moderately inhibited. In order to confirm part of the results obtained from in vitro experiments, in vivo experiments were also undertaken. The growth of HuH-7 cells transplanted subcutaneously into nude mice was significantly suppressed by intravenous injection of suramin. We discussed the possibility that certain types of human cancers, the growth of which seemed to be more or less dependent on polypeptide growth factors, might be sensitive to the antiproliferative effects of suramin.</p>
Keywords suramin anticancer drug human cancers
Amo Type Article
Published Date 1992-12
Publication Title Acta Medica Okayama
Volume volume46
Issue issue6
Publisher Okayama University Medical School
Start Page 457
End Page 463
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1485540
Web of Science KeyUT A1992KE49600008
JaLCDOI 10.18926/AMO/32635
FullText URL fulltext.pdf
Author Yerdel, Mehmet Ali| Moreira, Luis Fernando| Iwagaki, Hiromi| Kamikawa, Yasuaki| Tanaka, Noriaki| Orita, Kunzo|
Abstract <p>Acute superior mesenteric artery syndrome (SMAS) following a major surgical procedure is extremely rare, and represents an iatrogenic cause of postoperative upper gastrointestinal obstruction. In this report, the first documented case of acute SMAS following a left hemicolectomy is presented in an obese patient. Upper gastrointestinal roentgenographic series and conservative management remain to be the first line diagnostic and therapeutic modalities and were successful in our patient. Up to date no patient with SMAS reported to be obese but apparently obesity per se, can not be considered as an insurance. A postoperative acute SMAS is impossible to predict depending on the previous history, predisposing factors and the physique of the patient. Therefore, the surgeon should be aware of the SMAS and it is his task to secure all the precautions in order to preclude excessive traction on the mesenteric vasculature and vascular compression of the duodenum during surgery. In cases in which SMAS is suspected during extended colonic resections with lymph node dissection, duodenal mobilization seems to be selectively justifiable.</p>
Keywords superior mesenteric artery syndrome duodenal obstruction colectomy
Amo Type Article
Published Date 1992-12
Publication Title Acta Medica Okayama
Volume volume46
Issue issue6
Publisher Okayama University Medical School
Start Page 479
End Page 482
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1485543
Web of Science KeyUT A1992KE49600011
JaLCDOI 10.18926/AMO/32624
FullText URL fulltext.pdf
Author Zhang, Wei| Tanaka, Kotaro| Kunitomi, Keizo| Orita, Kunzo|
Abstract <p>Our initial experience with laparoscopic cholecystectomy for cholecystitis and cholelithiasis was reviewed in 42 patients and the data were compared with those of 21 patients who underwent conventional open cholecystectomy previously. Only one patient required conversion to an open operation. Three of the 42 patients had minor complications without death in laparoscopic cholecystectomy. The mean time for the laparoscopic cholecystectomy was 100 +/- 40 min, as compared with 79 +/- 21 min for the open cholecystectomy. The average postoperative hospital stay was 11.4 +/- 7.1 days for the laparoscopic procedure and 35.5 +/- 15.4 days for the conventional procedure. The laparoscopic cholecystectomy offers the patients shortened hospitalization and lower complications and can replace the conventional open cholecystectomy in large degree, at least in the uncomplicated cases.</p>
Keywords laparoscopic cholecystectomy cholecystitis cholelithiasis
Amo Type Article
Published Date 1992-08
Publication Title Acta Medica Okayama
Volume volume46
Issue issue4
Publisher Okayama University Medical School
Start Page 279
End Page 283
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1442152
Web of Science KeyUT A1992JL44200008
JaLCDOI 10.18926/AMO/32586
FullText URL fulltext.pdf
Author Orita, Kunzo| Kokumai, Yoshiaki| Kawada, Koichi| Kawahara, Toru| Takagi, Shigeru|
Abstract <p>A report is made on a case of liposarcoma of stomach in a 42 year old man. This is the first case of liposarcoma of stomach reported in Japan. The patient has remained asymptomatic for five years after operation.</p>
Amo Type Article
Published Date 1968-06
Publication Title Acta Medicinae Okayama
Volume volume22
Issue issue3
Publisher Okayama University Medical School
Start Page 167
End Page 173
NCID AA00041342
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4239074
NAID 120002311755
JaLCDOI 10.18926/AMO/32439
FullText URL fulltext.pdf
Author Miwa, Hiroaki| Orita, Kunzo|
Abstract <p>We studied the effects of a splenectomy in combination with immunotherapy on the survival of patients who had undergone a total gastrectomy. It was found that a splenectomy was not effective against advanced gastric cancer at stage III, and that the spleen should be retained for immunotherapy. Splenectomy for gastric cancer at terminal stage IV, particularly in combination with immunotherapy, produced not only augmentation of cellular immunity, but also increased survival.</p>
Keywords splenectomy immunotherapy levamisole cellular immunity survivalrate
Amo Type Article
Published Date 1983-06
Publication Title Acta Medica Okayama
Volume volume37
Issue issue3
Publisher Okayama University Medical School
Start Page 251
End Page 258
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6880832
Web of Science KeyUT A1983QW75400008
JaLCDOI 10.18926/AMO/32434
FullText URL fulltext.pdf
Author Horimi, Tadashi| Kagawa, Shigeo| Ninomiya, Motoki| Yoshida, Eiichi| Hiramtsu, Satoshi| Orita, Kunzo|
Abstract <p>That blood transfusions aid kidney graft survival is well known. Our data show that blood transfusions, except for the red blood cell component, promote growth of transplanted tumors in mice. These clinical and experimental observations suggest that blood transfusions may induce some immunological tolerance.</p>
Keywords blood transfusion immunological tolerance growth of transplanted tumors (in mice) blood component
Amo Type Brief Note
Published Date 1983-06
Publication Title Acta Medica Okayama
Volume volume37
Issue issue3
Publisher Okayama University Medical School
Start Page 259
End Page 263
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6880833
Web of Science KeyUT A1983QW75400009
JaLCDOI 10.18926/AMO/32404
FullText URL fulltext.pdf
Author Miwa, Hiroaki| Orita, Kunzo|
Abstract <p>Levamisole (LMS) was given to stage III gastric cancer patients starting three days before gastrectomy, at a does of 150 mg/day for three consecutive days every other week. Survival rates of these patients were compared with those of stage III gastric cancer patients previously operated in our Department who had not received levamisole. The background factors of both groups were matched as closely as possible. Both groups were concomitantly treated with mitomycin C and FT-207. The survival rate of the LMS group was significantly higher than that of the control group when the tumor had a diameter of 4.0-8.0 cm, cancer cells infiltrated to the gastric serosa, there were metastases within the regional lymph nodes, cancer cells slightly invaded the venous capillaren and there was moderate infiltration of the stroma.</p>
Keywords gastric cancer immunochemotherapy levamisole survival rate
Amo Type Article
Published Date 1983-12
Publication Title Acta Medica Okayama
Volume volume37
Issue issue6
Publisher Okayama University Medical School
Start Page 483
End Page 491
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6421090
Web of Science KeyUT A1983RW62800004
JaLCDOI 10.18926/AMO/32397
FullText URL fulltext.pdf
Author Hashimoto, Osamu| Miwa, Hiroaki| Orita, Kunzo|
Abstract <p>The effects of surgical intervention by removal of the primary focus, and the effectiveness of an immunomodulator, Corynebacterium parvum (Cp), on the proliferation of metastatic tumor tissue were investigated by following the postoperative changes in the 3H-thymidine labelling rate of metastatic tissue in an experimental model of metastasis in mice. In addition, the delayed type hypersensitivity reaction (DTH) was studied to investigate the immune capacity of the host. The labelling rate of mice that had the primary focus removed remained high with little variation, while that of the mice not operated on decreased gradually. On the other hand, in mice undergoing a sham operation, the rate was the same as that of the mice with the primary focus removed for a short while, but then gradually decreased. When Cp was administered, especially before removal of the primary focus, the rate was lower than that of the tumor bearing control group and decreased steadily. The number of pulmonary metastatic nodules was increased by removal of the primary focus, but this increase was inhibited by the administration of Cp which prolonged life. The depression in the DTH was less in the group given Cp preoperativeLy than in either the group of mice having the primary focus removed or those not having it removed.</p>
Keywords metastatic tumor surgical intervension autoradiography Corynebacterium parvum
Amo Type Article
Published Date 1983-08
Publication Title Acta Medica Okayama
Volume volume37
Issue issue4
Publisher Okayama University Medical School
Start Page 335
End Page 340
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6624533
Web of Science KeyUT A1983RE57600006
JaLCDOI 10.18926/AMO/32396
FullText URL fulltext.pdf
Author Ono, Minoru| Tanaka, Noriaki| Orita, Kunzo|
Abstract <p>Antibody-dependent cellular cytotoxicity (ADCC) increased with the development of tumors in C3H/He mice bearing spontaneous breast cancer or the syngeneic hepatoma MH-134 and in C57BL/6 mice bearing the syngeneic Lewis lung carcinoma 3LL. This cytotoxicity decreased after treatment with guinea pig, monoclonal IgM anti-Thy 1.2 serum and complement to the non-cancer level thus indicating that the increased ADCC in mice with cancer seems mainly attributable to cells with the Thy 1 antigen. On the other hand, NK activity decreased greatly when mice had tumors. Treatment with monoclonal IgM anti-Thy 1.2 serum and complement showed no significant influence on the natural killer (NK) activity of spleen cells of mice bearing MH-134 cancer, but in the 3LL-bearing mice the activity decreased significantly.</p>
Keywords ADCC acitivity NK activity Thy 1 antigen plastic dish fractionation tumor-bearing mice
Amo Type Article
Published Date 1983-08
Publication Title Acta Medica Okayama
Volume volume37
Issue issue4
Publisher Okayama University Medical School
Start Page 367
End Page 376
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6605026
Web of Science KeyUT A1983RE57600009
JaLCDOI 10.18926/AMO/32201
FullText URL fulltext.pdf
Author Shiiki, Sigeo| Fuchimoto, Sadanori| Iwagaki, Hiromi| Akazai, Yoshihiro| Matsubara, Nagahide| Watanabe, Tetsuya| Orita, Kunzo|
Abstract <p>We investigated the antitumor activities of 5-fluorouracil (5-FU), 5'-deoxy-5-fluorouridine (5'-DFUR), 1-hexylcarbamoyl-5-fluorouracil (HCFU) and 1-(tetrahydro-2-furanyl)-5-fluorouracil (FT-207) in combination with hyperthermia in vitro. The antitumor effect of 5-FU (10(-4) M) was slightly enhanced by combination with hyperthermia (42 degrees C) for 2h, and the effect was determined to be additive. Synergistic enhancement of antitumor activity was obtained by the concurrent use of hyperthermia (42 degrees C, 2h) and 5'-DFUR (10(-4) M) or HCFU (10(-5) M). However, the antitumor effect of FT-207 (10(-4) M) in combination with hyperthermia was comparable that of hyperthermia alone. The synergistic enhancement of antitumor activity was not obtained for all drugs when the cells were preheated at 42 degrees C for 2h. On the other hand, when cells were pretreated with drugs before heat exposure, weak interactions were obtained after 5-FU and 5'-DFUR treatment, and a synergistic interaction was obtained after HCFU treatment. It is speculated that the metabolites of 5'-DFUR and HCFU enhance the cytotoxicity of 5-FU, or might change the threshold concentration for a cytotoxic effect of 5-FU in cancer cells.</p>
Keywords hyperthermia 5-fluorouridine 5'-deoxy-5-fluorouridine 1-hexylcarbomoyl-5-fluorouracil FT-207
Amo Type Article
Published Date 1991-10
Publication Title Acta Medica Okayama
Volume volume45
Issue issue5
Publisher Okayama University Medical School
Start Page 339
End Page 345
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1836706
Web of Science KeyUT A1991GN53800008
JaLCDOI 10.18926/AMO/32083
FullText URL fulltext.pdf
Author Miwa, Hiroaki| Orita, Kunzo|
Abstract <p>Levamisole was administered to 177 patients with gastrointestinal cancer (88 curative resection, 58 noncurative resection and 31 without resection). It was administered at a daily dose of 150 mg for three consecutive days every other week. The administration was started, as a rule, 3 days before operation. This medication was repeated as frequently as possible at least for one month. The cellular immunity and 18-month survival rate of treated and control groups were compared. Levamisole effectively improved peripheral lymphocyte blastformation against phytohemagglutinin and increased the numbers of peripheral blood lymphocytes. Levamisole caused extremely high blastformation rates, in general, enhanced PPD reactions in non-curative resection cases 7 months after operation and showed no influence upon the number of peripheral blood lymphocyte. The effect of levamisole on the 6-month survival rate was most marked in patients without resection. Increased 12-month survival rate was marked in non-curative resection cases and, to a lesser extent, curative resection cases. Patients without resection had a slightly improved 12-month survival rate. Levamisole improved the 18-month survival rate in resectable cases; however, there were no significant differences in 18-month survival between levamisole and control groups of patients not undergoing resection. The results suggest that levamisole is effective in the patients whose tumor cells have been decreased by any method.</p>
Keywords levamisole. gastrointestinal cancer cell-mediated immunity survival rate
Amo Type Article
Published Date 1979-02
Publication Title Acta Medica Okayama
Volume volume33
Issue issue1
Publisher Okayama University Medical School
Start Page 29
End Page 42
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 155980
JaLCDOI 10.18926/AMO/31939
FullText URL fulltext.pdf
Author Sakagami, Kenichi| Takeuchi, Hitoshi| Tsuboi, Katsutoshi| Matsumoto, Takamasa| Tanaka, Kohtaro| Ohsaki, Toshihide| Horimi, Tadashi| Fuchimoto, Sadanori| Orita, Kunzo|
Abstract <p>The survival rate of 19 patients who underwent living-related kidney transplantation after donor-specific blood transfusions (DST) was compared with that of 32 historical controls receiving transplants without DST. The graft survival rate of the DST group was 82% after two and three years. The graft survival rate of the DST group was significantly better than the 53% rate after two years obtained with the 32 historical controls (p less than 0.05). We tested sera from 16 DST-treated recipients to study the beneficial effect of DST on kidney allograft survival using the mixed lymphocyte culture (MLC) serum inhibition test. The results demonstrated that MLC inhibitory factors were induced in the serum of the recipient after completion of DST. This inhibition of MLC was observed by treatment of responder lymphocytes with serum obtained three weeks after DST plus rabbit complement. The inhibitory effect was also specific for responder cells in anti-donor MLC. Regarding the correlation with rejection episodes, these MLC inhibitory factors were often observed in the non-rejection group (p less than 0.05). The data suggest that such factors may be anti-idiotypic antibodies and be associated with prolonged graft survival.</p>
Keywords kidney transplantation donor-specific blood transfusion (DST) MLC inhibitory factors anti-idiotypic antibody.
Amo Type Article
Published Date 1986-02
Publication Title Acta Medica Okayama
Volume volume40
Issue issue1
Publisher Okayama University Medical School
Start Page 39
End Page 43
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2938423
Web of Science KeyUT A1986A190200006
JaLCDOI 10.18926/AMO/31928
FullText URL fulltext.pdf
Author Sakagami, Kenichi| Miyazaki, Masashi| Matsuoka, Junji| Shiozaki, Shigehiro| Saito, Shinya| Orita, Kunzo|
Abstract <p>An artificial liver support system for plasma exchange and plasma perfusion through BR-601 resin using a membrane separator was applied to 5 patients with postoperative liver failure. Percent absorption of total and direct bilirubin, and of bile acids were 77.1 +/- 6.4, 78.4 +/- 6.1, and 93.4 +/- 3.6%, respectively, when 250 ml of plasma was treated. Percent reductions in total and direct bilirubin, and in bile acids were 24.5 +/- 5.8, 25.5 +/- 5.8 and 30.9 +/- 8.5%, respectively. In contrast, percent reductions in total and direct bilirubin, and in bile acids by plasma exchange were 30.9 +/- 13.3, 34.5 +/- 12.5 and 24.2 +/- 8.5%, respectively. The coma grade was improved in 4 out of 5 cases, but unfortunately the patients did not recover. In conclusion, plasma perfusion through BR-601 resin is expected to play a promising role in artificial liver support systems because of its capacity to absorb bilirubin and bile acids.</p>
Keywords anion exchange resin (BR-601) postoperative liver failure artificial liver support
Amo Type Article
Published Date 1986-10
Publication Title Acta Medica Okayama
Volume volume40
Issue issue5
Publisher Okayama University Medical School
Start Page 249
End Page 255
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3788665
Web of Science KeyUT A1986E557800003
JaLCDOI 10.18926/AMO/31919
FullText URL fulltext.pdf
Author Yoshihara, Hisashi| Tanaka, Noriaki| Orita, Kunzo|
Abstract <p>The influence of surgical stress on the natural killer (NK) activity of peripheral blood lymphocytes in patients with carcinoma of the lung or gastrointestinal system was studied. The peripheral blood lymphocytes of the patients showed a marked decrease in NK activity against K-562 cells as target cells 1-2 days after surgery. The activity remained lowered for 2 weeks after thoractomy and for 1 week after laparotomy. No appreciable suppression of NK activity was observed with normal human peripheral blood lymphocytes preincubated with postoperative patient sera. Peripheral blood mononuclear cells obtained postoperatively from patients lost NK activity after ultraviolet irradiation, without any detectable loss of viability. Such irradiated mononuclear cells showed inhibition of NK activity after a 24-hour preincubation with peripheral blood lymphocytes from normal subjects. Similar suppressive activity was demonstrable in a fraction of mononuclear cells with adhesiveness to plastic petri dishes, while non-adherent cells had no such activity. When added immediately to the cytotoxicity assay system without the 24-hour preincubation, patient mononuclear cells caused no inhibition of NK activity, whereas adherent cells from normal subjects enhanced NK activity. The findings seems to indicate that, following surgical stress, plastic dish-adherent peripheral blood mononuclear cells become deprived of NK helper activity and exert suppression, thus causing postoperative depression of NK activity.</p>
Keywords natural killing suppressor cell surgical stress
Amo Type Article
Published Date 1986-04
Publication Title Acta Medica Okayama
Volume volume40
Issue issue2
Publisher Okayama University Medical School
Start Page 113
End Page 119
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2940814
Web of Science KeyUT A1986C034800007
JaLCDOI 10.18926/AMO/31911
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Author Takiwa, Takayoshi| Miyagiwa, Miki| Kawai, Akira| Hamazaki, Keisuke| Mimura, Hisashi| Orita, Kunzo| Sato, Jiro|
Abstract <p>Primary cultures of surgically obtained noncancerous portions of human liver tissues were made. Liver tissues were poorly dissociated with collagenase, but well dissociated with dispase. The yield and viability of cells were improved somewhat when dissociated with collagenase followed by dispase. The mean cell yield was 1.1 X 10(6) cells/g liver. The epithelial-like morphology of the dissociated liver cells was maintained for about one week, but thereafter degenerative alteration of cells was observed. In liver explant culture, an active outgrowth of cells was observed for more than one month. Albumin production in culture fluids from dissociated livers was detectable for about 2 weeks, but later became undetectable, while that from explant culture was detectable for at least one month. These data demonstrate that adult human hepatocytes can be isolated from noncancerous portions of livers with relatively high yield, and that albumin production of the dissociated cells is detectable for several days.</p>
Keywords human liver cell culture enzymatic dissociation explant culture albumin production
Amo Type Article
Published Date 1986-08
Publication Title Acta Medica Okayama
Volume volume40
Issue issue4
Publisher Okayama University Medical School
Start Page 209
End Page 213
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3020891
Web of Science KeyUT A1986D870500005