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Author
Sing’ombe Ombiro Geofrey Graduate School of Environmental and Life Science, Okayama University
Sawai, Taku Graduate School of Environmental and Life Science, Okayama University
Noutoshi, Yoshiteru Graduate School of Environmental and Life Science, Okayama University
Nishina, Yuta Research Core for Interdisciplinary Sciences, Okayama University
Matsui, Hidenori Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life Science, Okayama University
Toyoda, Kazuhiro Graduate School of Environmental and Life Science, Okayama University
Ichinose, Yuki Graduate School of Environmental and Life Science, Okayama University
Abstract
Plant pathogenic bacteria cause huge yield losses in crops globally. Therefore, finding effective bactericides to these pathogens is an immediate challenge. In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at >5 μg/ml final concentration. The most effective compounds, ralhibitins A, C, and E completely inhibited the growth of Rs1002 at 1.25 μg/ml. In addition, ralhibitins A to E inhibited growth of Xanthomonas oryzae pv. oryzae but not the other bacteria tested at a final concentration of 10 μg/ml. Whereas, ralhibitin E, besides inhibiting R. solanacearum and X. oryzae pv. oryzae, completely inhibited the growth of X. campestris pv. campestris and the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis at 10 μg/ml. Growth inhibition by these compounds was stable at pH 6–9 and after autoclaving. Because Rs1002 grew in the culture medium in which ralhibitins were incubated with the ralhibitin-insensitive bacteria, the unaffected bacteria may be able to inactivate the inhibitory effect of ralhibitins. These results suggest that ralhibitins might be potential lead compounds for the specific control of phytopathogenic bacteria.
Keywords
Bactericide
Ralhibitins
Ralstonia solanacearum
Lead compounds
Note
This is an Accepted Manuscript of an article published by Elsevier
This fulltext will be available in June 2019
Published Date
2018-10
Publication Title
Microbiological Research
Volume
volume215
Publisher
Elsevier
Start Page
29
End Page
35
ISSN
0944-5013
NCID
AA11017780
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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isVersionOf https://doi.org/10.1016/j.micres.2018.06.005