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ID 34248
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Author
Sakamura, Sho
Ushijima, Takayuki
Hama, Yoichiro
Kajiura, Hiroyuki
Fujiyama, Kazuhito
Okihara, Kiyoshi
Hashimoto, Ken
Sugimoto, Hiroyuki
Yamada, Hideo
Abstract
In a previous study (Y. Kimura et al., Biosci. Biotechnol Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Ga1 beta 1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new beta 1-3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such beta 1-3galactosyltransferase activity, which can transfer galactosyl residues with the beta 1-3 linkage to beta 1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc(2)GlcNAc(2)Man(3)-GlcNAc(2)-PA as acceptor N-glycan, we detected the beta 1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique beta 1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.
Keywords
royal jelly glycoprotein; N-glycan
Thomsen-Friedenreich antigen (Gal beta 1-3GalNAc)
beta 1-3 galactosyltransferase
Apis mellifera
Note
Published with permission from the copyright holder.
This is published in Bioscience Biotechnology and Biochemistry, 2007 Vol.71 Issue.4 pp.1111-1114
Publisher URL: http://dx.doi.org/10.1271/bbb.70081
Direct access to Thomson Web of Science record
(C)2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Published Date
2007-04
Publication Title
Bioscience, Biotechnology, and Biochemistry
Volume
volume71
Issue
issue4
Publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry
Publisher Alternative
日本農芸化学会
Start Page
1111
End Page
1114
ISSN
0916-8451
NCID
AA10824164
Content Type
Journal Article
language
英語
Copyright Holders
Japan Society for Bioscience, Biotechnology and Agrochemistry
File Version
publisher
Refereed
True
DOI
Web of Sience KeyUT
Submission Path
biochemistry/18