Author Zamami, Yoshito| Takatori, Shingo| Goda, Mitsuhiro| Koyama, Toshihiro| Iwatani, Yukiko| Jin, Xin| Takada-Doi, Shima| Kawasaki, Hiromu|
Published Date 2008-11-01
Publication Title Biological & Pharmaceutical Bulletin
Volume volume31
Issue issue11
Content Type Journal Article
JaLCDOI 10.18926/AMO/30965
FullText URL fulltext.pdf
Author Jin, Xin| Otonashi-Satoh, Yukiko| Sun, Pengyuan| Kawamura, Naomi| Tsuboi, Takashi| Yamaguchi, Yasuyo| Ueda, Taro| Kawasaki, Hiromu|
Abstract <p>The vascular effects of an aqueous extract prepared from the leaves of Eucommia ulmoides Oliv. (ELE), a medicinal herb commonly used in antihypertensive herbal prescriptions in China, were investigated in rat mesenteric resistance arteries. The mesenteric vascular bed was perfused with Krebs solution and the perfusion pressure was measured with a pressure transducer. In preparations with an intact endothelium and precontracted with 7&#956;M methoxamine, perfusion of ELE (10<sup>&#65293;7</sup>&#65293;10<sup>&#65293;2</sup>mg/ml for 15min) caused a concentration-dependent vasodilatation, which was abolished by chemical removal of the endothelium. The ELE-induced vasodilatation was inhibited by neither indomethacin (INDO, a cyclooxygenase inhibitor) nor N<sup>G</sup>-nitro-L-arginine-methyl ester (L-NAME, a nitric oxide inhibitor). The ELE-induced vasodilatation was significantly inhibited by tetraethylammonium (TEA, a K<sup>+</sup> channel blocker) and 18&#945;-glycyrrhetinic acid (18&#945;-GA, a gap-junction inhibitor), and abolished by high K<sup>+</sup> -containing Krebs&#700; solution. Atropine (a muscarinic acetylcholine receptor antagonist) significantly inhibited the vasodilatation induced by ELE at high concentrations. These results suggest that the ELE-induced vasodilatation is endothelium-dependent but nitric oxide (NO)- and prostaglandin I<sub>2</sub> (PGI<sub>2</sub>)-independent, and is mainly mediated by the endothelium-derived hyperpolarizing factor (EDHF) in the mesenteric resistance arteries. Furthermore, the ELE-induced EDHF-mediated response involves the activation of K<sup>+</sup>-channels and gap junctions.</p>
Keywords Eucommia ulmoides Oliv. leaf extract endothelium-dependent vasodilation endothelium-derived hyperpolarizing factor mesenteric resistance artery
Amo Type Original Article
Published Date 2008-10
Publication Title Acta Medica Okayama
Volume volume62
Issue issue5
Publisher Okayama University Medical School
Start Page 319
End Page 325
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18985092
Web of Science KeyUT 000260391300006