JaLCDOI 10.18926/AMO/30417
FullText URL fulltext.pdf
Author Jahan, Israt| Bai, Liyan| Iijima, Mikio| Kondo, Tadashi| Namba, Masayoshi|
Abstract <p>The establishment of a model system of neoplastic transformation of normal human cells has been attempted with a chemical carcinogen, 4-nitroquinoline 1-oxide (4NQO). In the course of these experiments, it was noticed that immortalization of human cells is a multi-step process involving several mutational genetic events. Thus, chromosomal changes which occurred during the process of immortalization of human fibroblasts were examined. To accomplish immortalization, fibroblasts obtained from an embryo were repeatedly treated with 10<sub>-6</sub>M4NQO from primary culture to passage 51 (59 treatments in total). Before immortalization, some chromosomes (especially, chromosomes 2, 6, 8, 10, 11, 12, 15, 19, and 20), were lost at a relatively high frequency. After immortalization, the chromosomes distributed so broadly in the triploid to hypotetraploid region without a distinct modal number or without marker chromosomes that it was difficult to identify the specific chromosomes related to the immortalization of human cells. No specific structural chromosomal changes were detected. Although the significance of such chromosome changes in relation to immortalization is not clear, the loss of some specific chromosomes suggests that genes which are involved in cellular aging and which suppress immortalization may have been lost in the immortalization process.</p>
Keywords human cells chromosomes aging immortalization 4NQO
Amo Type Article
Published Date 1995-02
Publication Title Acta Medica Okayama
Volume volume49
Issue issue1
Publisher Okayama University Medical School
Start Page 25
End Page 28
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7762406
Web of Science KeyUT A1995QK32500004