start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=1-2
article-no=
start-page=123
end-page=129
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of temperature on the stimulation of steroid secretion in murine adrenal tumor cells (Y-1 clone)
kn-title=温度変化がマウス副腎皮質腫瘍由来細胞（Y-1クローン）のステロイドホルモン分泌能に与える影響に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The stimulation of steroidogenesis by adrenocorticotropic hormone (ACTH) and adenosine 3'-5' monophosphate (cyclic-AMP) was investigated in in vitro systems. Temperature is one of the most important elements for cell culture and affects various biological cell functions. In this study, the effects of various temperatures (32-43°C) on the stimulation of steroidogenesis by ACTH and cyclic AMP in the murine adrenal tumor cell (Y-1) were investigated. After Y-1 cells were cultured at 32-43°C for 48 hours, most of the population survived. Y-1 was insensitive to high temperature during 48 hours. When cells were incubated at 32°C, steroids in the medium could not be detected, but at 33-39°C, steroid amounts increased linearly with increasing temperature. At 41°C or higher, steroid secretion decreased drastically. Although the stimulation of steroidogenesis by ACTH was accelerated sharply at 33°C, stimulation by cyclic AMP may be gradually increased until 39°C. These data suggest that this may be caused by different action sites of ACTH and cyclic AMP. More studies indicated that ACTH binds to the plasma membrane and activated adenyl cyclase, but cyclic AMP function required binding to the specific cytosol receptor.
en-copyright=
kn-copyright=

en-aut-name=Ichikawa (Azuma)Yoshiko
en-aut-sei=Ichikawa (Azuma)
en-aut-mei=Yoshiko
kn-aut-name=市川淑子
kn-aut-sei=市川
kn-aut-mei=淑子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌源研究施設病理部門
en-keyword=Y-1
kn-keyword=Y-1
en-keyword=ステロイドホルモン
kn-keyword=ステロイドホルモン
en-keyword=ACTH
kn-keyword=ACTH
en-keyword=cyclic AMP
kn-keyword=cyclic AMP
en-keyword=温度感受性
kn-keyword=温度感受性
END