JaLCDOI 10.18926/AMO/31950
FullText URL fulltext.pdf
Author Wu, Di| Miyamoto, Osamu| Shibuya, Sei| Okada, Maiko| Igawa, Hiroharu| Janjua, Najima A.| Norimatsu, Hiromichi| Itano, Toshifumi|
Abstract <p>Macrophages and microglia are implicated in spinal cord injury, but their precise role is not clear. In the present study, activation of these cells was examined in a spinal cord injury model using 2 different antibodies against ED1 clone and ionized calcium binding adaptor molecule 1 (Iba1). Activation was observed at 1, 4, 8, and 12 weeks after contusion injury and was compared with sham operated controls. Our results indicate that activation could be observed in both the dorsal funiculus and the ventral white matter area in the spinal cord at 5 mm rostral to the epicenter of injury. For both cells, there was a gradual increase in activation from 1-4 weeks, followed by down-regulation for up to 12 weeks. As a result, we could stain macrophages by ED1 and microglia by Iba1. We concluded that macrophages may play a role in the phagocytosis of denatured dendrites after spinal cord injury, while microglia may have some cooperative functions, as they were found scattered near the macrophages.</p>
Keywords macrophages microglia spinal cord injury ED1 Iba1
Amo Type Article
Published Date 2005-08
Publication Title Acta Medica Okayama
Volume volume59
Issue issue4
Publisher Okayama University Medical School
Start Page 121
End Page 127
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16155637
Web of Science KeyUT 000231478000002