JaLCDOI 10.18926/AMO/30308
FullText URL fulltext.pdf
Author Taketa, Kazuhisa| Ichikawa, Eriko| Taga, Hiroko| Hirai, Hidematsu|
Abstract <p>&lt;p&gt;A combination of agarose gel electrophoresis and a newly developed technique of electro-affinity transfer was applied to the detection of circulating immune complexes of human alpha-fetoprotein (AFP) and anti-AFP. After electrophoretic transfer to nitrocellulose membrane, to which affinity-purified polyclonal horse antibodies to human AFP were bound, the membranes were treated with or without rabbit immunoglobulins to human AFP, followed by overlaying with horseradish peroxidase-labeled goat anti-rabbit IgG for color development. Artificial complexes formed in vitro from human AFP and rabbit anti-AFP were clearly separated from free AFP by the agarose electrophoresis. The complexes were stained 20-40% as dark as the equivalent amount of free AFP by treatment with rabbit anti-AFP, and 10-20% as dark without the antibody treatment over a wide range of antigen-antibody ratios.&lt;/p&gt;</p>
Keywords circulating immune complexes ?-fetoprotein electrophoresis electroaffinity transfer enzyme immuno-detection
Amo Type Brief Note
Published Date 1984-08
Publication Title Acta Medica Okayama
Volume volume38
Issue issue4
Publisher Okayama University Medical School
Start Page 409
End Page 413
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6208755
Web of Sience KeyUT A1984TG25900011