JaLCDOI 10.18926/AMO/31729
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Author Ichiyasu, Akira| Takaoka, Michio| Makino, Hirofumi| Takahashi, Kayo| Ota, Zensuke|
Abstract <p>Rat kidney glomerular basement membrane (GBM) was isolated and digested with alpha-amylase and elastase. Electron microscopy revealed a meshwork structure composed of fibrils 3 nm in width. They appeared to be type IV collagen fibrils. We succeeded in clarifying a significant ultrastructural aspect of the GBM which had been unclear until now. The findings are consistent with our previously proposed GBM molecular sieve theory.</p>
Keywords type IV collagen glomerular basement membrane enzymatic digestion fibrillar ultrastructure
Amo Type Article
Published Date 1987-08
Publication Title Acta Medica Okayama
Volume volume41
Issue issue4
Publisher Okayama University Medical School
Start Page 183
End Page 185
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3499055
Web of Sience KeyUT A1987J833200007
JaLCDOI 10.18926/AMO/31004
FullText URL fulltext.pdf
Author Ichiyasu, Akira| Makino, Hirofumi|
Abstract <p>Isolated rat glomerular basement membrane was treated with elastase and observed by transmission electron microscopy. The treatment with elastase revealed the fundamental structure of the glomerular basement membrane quite clearly, and enabled the observation of a sieve structure within the glomerular basement membrane. This sieve structure may play a major role in the filtration of blood as well as in the production of urine. Treatment with antibody showed that the sieve was mainly constituted of type IV collagen.</p>
Keywords glomerular basement membrane enzymatic digestion meshwork structure type IV collagen elastase
Amo Type Article
Published Date 1988-12
Publication Title Acta Medica Okayama
Volume volume42
Issue issue6
Publisher Okayama University Medical School
Start Page 317
End Page 325
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2467525
Web of Sience KeyUT A1988R743300003